Cortisol antibody | C13 (BGN/C13)

Mouse anti Cortisol

Product Type
Monoclonal Antibody
Clone
C13 (BGN/C13)
Isotype
IgG3
Specificity
Cortisol

Product Code Applications Pack Size List Price Your Price Qty
2330-4809
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.2 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti cortisol monoclonal antibody, clone C13 recognizes cortisol, a glucocorticoid hormone synthesized from cholesterol by the adrenal cortex. It is released in response to pituitary adenocorticotrophic hormone (ACTH) which is, in turn, regulated by corticotrophin releasing factor (CRF). High levels of cortisol act to regulate the release of ACTH and CRF via negative feedback inhibition.
The release of cortisol stimulates the conversion of proteins to carbohydrates, raises blood sugar levels and promotes glycogen storage in the liver.

The following cross reactivities were observed:
Cortisol100%
Prednisolone30%
Cortisone25%
Corticosterone10%
11-desoxycortisol5%

No reactivity was observed with 17-a-OH-progesterone.

Target Species
Broad
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Cortisol conjugated to bovine serum albumin.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/50 1/1500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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References for Cortisol antibody

  1. Arya, S.K. et al. (2010) Antibody functionalized interdigitated micro-electrode (IDmicroE) based impedimetric cortisol biosensor.
    Analyst. 135 (8): 1941-6.
  2. Arya, S.K. and Bhansali, S. (2012) Anti-Prostate Specific Antigen (Anti-PSA) Modified Interdigitated Microelectrode-Based Impedimetric Biosensor for PSA Detection
    Biosensors Journal 1: H110601
  3. Arya, S.K. et al. (2010) Dithiobis(succinimidyl propionate) modified gold microarray electrode based electrochemical immunosensor for ultrasensitive detection of cortisol.
    Biosens Bioelectron. 25: 2296-301.
  4. Arya, S.K. et al. (2011) Polyaniline protected gold nanoparticles based mediator and label free electrochemical cortisol biosensor.
    Biosens Bioelectron. 28 (1): 166-73.
  5. Kaushik, A. et al. (2013) Mediator and label free estimation of stress biomarker using electrophoretically deposited Ag@AgO-polyaniline hybrid nanocomposite.
    Biosens Bioelectron. 50: 35-41.

RRID
AB_618933
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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