Cortisol antibody | C13 (BGN/C13)
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Mouse anti cortisol monoclonal antibody, clone C13 recognizes cortisol, a glucocorticoid hormone synthesized from cholesterol by the adrenal cortex. It is released in response to pituitary adenocorticotrophic hormone (ACTH) which is, in turn, regulated by corticotrophin releasing factor (CRF). High levels of cortisol act to regulate the release of ACTH and CRF via negative feedback inhibition.
The release of cortisol stimulates the conversion of proteins to carbohydrates, raises blood sugar levels and promotes
glycogen storage in the liver.The following cross reactivities were observed:
No reactivity was observed with 17-a-OH-progesterone.
| Cortisol | 100% |
| Prednisolone | 30% |
| Cortisone | 25% |
| Corticosterone | 10% |
| 11-desoxycortisol | 5% |
Product Details
- Target Species
- Broad
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Carrier Free
- Yes
- Immunogen
- Cortisol conjugated to bovine serum albumin.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of Cortisol antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/50 | 1/1500 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Cortisol antibody
-
Arya, S.K. et al. (2010) Antibody functionalized interdigitated micro-electrode (IDmicroE) based impedimetric cortisol biosensor.
Analyst. 135 (8): 1941-6. -
Arya, S.K. and Bhansali, S. (2012) Anti-Prostate Specific Antigen (Anti-PSA) Modified Interdigitated Microelectrode-Based Impedimetric Biosensor for PSA Detection
Biosensors Journal 1: H110601 -
Arya, S.K. et al. (2010) Dithiobis(succinimidyl propionate) modified gold microarray electrode based electrochemical immunosensor for ultrasensitive detection of cortisol.
Biosens Bioelectron. 25: 2296-301. -
Arya, S.K. et al. (2011) Polyaniline protected gold nanoparticles based mediator and label free electrochemical cortisol biosensor.
Biosens Bioelectron. 28 (1): 166-73. -
Kaushik, A. et al. (2013) Mediator and label free estimation of stress biomarker using electrophoretically deposited Ag@AgO-polyaniline hybrid nanocomposite.
Biosens Bioelectron. 50: 35-41.
