Cortisol antibody | C13 (BGN/C13)

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Mouse anti Cortisol

Product Type
Monoclonal Antibody
Clone
C13 (BGN/C13)
Isotype
IgG3
Product Code Applications Pack Size List Price Quantity
2330-4809
Datasheet
E
MSDS
0.2 mg loader

Mouse anti cortisol monoclonal antibody, clone C13 recognizes cortisol, a glucocorticoid hormone synthesized from cholesterol by the adrenal cortex. It is released in response to pituitary adenocorticotrophic hormone (ACTH) which is, in turn, regulated by corticotrophin releasing factor (CRF). High levels of cortisol act to regulate the release of ACTH and CRF via negative feedback inhibition.
The release of cortisol stimulates the conversion of proteins to carbohydrates, raises blood sugar levels and promotes glycogen storage in the liver.

The following cross reactivities were observed:
Cortisol100%
Prednisolone30%
Cortisone25%
Corticosterone10%
11-desoxycortisol5%

No reactivity was observed with 17-a-OH-progesterone.

Product Details

Target Species
Broad
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Cortisol conjugated to bovine serum albumin.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Cortisol antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/50 1/1500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG3:FITC HCA039F F 0.1 mg loader
Human anti Mouse IgG3:HRP HCA039P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Application Based External Images

ELISA

Product Specific References

References for Cortisol antibody

  1. Arya, S.K. et al. (2010) Antibody functionalized interdigitated micro-electrode (IDmicroE) based impedimetric cortisol biosensor.
    Analyst. 135 (8): 1941-6.
  2. Arya, S.K. and Bhansali, S. (2012) Anti-Prostate Specific Antigen (Anti-PSA) Modified Interdigitated Microelectrode-Based Impedimetric Biosensor for PSA Detection
    Biosensors Journal 1: H110601
  3. Arya, S.K. et al. (2010) Dithiobis(succinimidyl propionate) modified gold microarray electrode based electrochemical immunosensor for ultrasensitive detection of cortisol.
    Biosens Bioelectron. 25: 2296-301.
  4. Arya, S.K. et al. (2011) Polyaniline protected gold nanoparticles based mediator and label free electrochemical cortisol biosensor.
    Biosens Bioelectron. 28 (1): 166-73.
  5. Kaushik, A. et al. (2013) Mediator and label free estimation of stress biomarker using electrophoretically deposited Ag@AgO-polyaniline hybrid nanocomposite.
    Biosens Bioelectron. 50: 35-41.