Troponin T antibody | T1/61
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Mouse anti Chicken Troponin T antibody, clone T1/61 recognizes chicken Troponin T, fast skeletal muscle isoforms, also known as fast muscle Troponin T. Troponin T is a 262 amino acid tropomyosin binding component of the troponin complex with a predicted molecular mass of ~31 kDa.
Four isoforms of Troponin T generated by alternative splicing have been described. Mouse anti Chicken Troponin T antibody, clone T1/61 recognizes the canonical isoform 1, binding to othe potential isoforms has not been evaluated (UniProt : P12620).
Four isoforms of Troponin T generated by alternative splicing have been described. Mouse anti Chicken Troponin T antibody, clone T1/61 recognizes the canonical isoform 1, binding to othe potential isoforms has not been evaluated (UniProt : P12620).
Product Details
- Target Species
- Chicken
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rabbit Human Quail - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Troponin T, purified from chicken breast muscle
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P12620 Related reagents
- Entrez Gene
- TNNT3 Related reagents
- Regulatory
- For research purposes only
Applications of Troponin T antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Immunohistology
- This product does not require protein digestion pre-treatment of paraffin sections.This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
- Histology Positive Control Tissue
- Skeletal muscle
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Troponin T antibody
-
Bird, I.M. et al. (1985) Identification of multiple variants of fast muscle troponin T in the chicken using monoclonal antibodies.
Eur J Biochem. 150 (3): 517-25. -
Watkins, H. et al. (1996) Expression and functional assessment of a truncated cardiac troponin T that causes hypertrophic cardiomyopathy. Evidence for a dominant negative action.
J Clin Invest. 98: 2456-61. -
Marian, A.J. et al. (1997) Expression of a mutant (Arg92Gln) human cardiac troponin T, known to cause hypertrophic cardiomyopathy, impairs adult cardiac myocyte contractility.
Circ Res. 81: 76-85. -
Dunnick, J.K. et al. (2004) Cardiac damage in rodents after exposure to bis(2-chloroethoxy)methane.
Toxicol Pathol. 32: 309-17. -
Herman, E.H. et al. (1999) Correlation between serum levels of cardiac troponin-T and the severity of the chronic cardiomyopathy induced by doxorubicin.
J Clin Oncol. 17: 2237-43. -
Zhang, J. et al. (2008) Isoproterenol-induced cardiotoxicity in sprague-dawley rats: correlation of reversible and irreversible myocardial injury with release of cardiac troponin T and roles of iNOS in myocardial injury.
Toxicol Pathol. 36: 277-8. -
Sweeney, H.L. et al. (1998) Functional analyses of troponin T mutations that cause hypertrophic cardiomyopathy: insights into disease pathogenesis and troponin function.
Proc Natl Acad Sci U S A. 95: 14406-10. -
Zhang, J. et al. (2006) Mechanisms and biomarkers of cardiovascular injury induced by phosphodiesterase inhibitor III SK&F 95654 in the spontaneously hypertensive rat.
Toxicol Pathol. 34: 152-63. -
Baharom, F. et al. (2016) Dendritic Cells and Monocytes with Distinct Inflammatory Responses Reside in Lung Mucosa of Healthy Humans.
J Immunol. 196 (11): 4498-509.
