Troponin T antibody | T1/61
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Mouse anti Chicken Troponin T antibody, clone T1/61 recognizes chicken Troponin T, fast skeletal muscle isoforms, also known as fast muscle Troponin T. Troponin T is a 262 amino acid tropomyosin binding component of the troponin complex with a predicted molecular mass of ~31 kDa.
Four isoforms of Troponin T generated by alternative splicing have been described. Mouse anti Chicken Troponin T antibody, clone T1/61 recognizes the canonical isoform 1, binding to othe potential isoforms has not been evaluated (UniProt : P12620).
Four isoforms of Troponin T generated by alternative splicing have been described. Mouse anti Chicken Troponin T antibody, clone T1/61 recognizes the canonical isoform 1, binding to othe potential isoforms has not been evaluated (UniProt : P12620).
Product Details
- Target Species
- Chicken
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rabbit Human Quail - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Troponin T, purified from chicken breast muscle
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
Applications of Troponin T antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Immunohistology
- This product does not require protein digestion pre-treatment of paraffin sections.This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
- Histology Positive Control Tissue
- Skeletal muscle
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Secondary Antibodies Available
Product Specific References
References for Troponin T antibody
-
(2011) Human Protein Atlas: CAB000472
Human Protein Atlas: CAB000472 -
Bird, I.M. et al. (1985) Identification of multiple variants of fast muscle troponin T in the chicken using monoclonal antibodies.
Eur J Biochem. 150 (3): 517-25. -
Watkins, H. et al. (1996) Expression and functional assessment of a truncated cardiac troponin T that causes hypertrophic cardiomyopathy. Evidence for a dominant negative action.
J Clin Invest. 98: 2456-61. -
Marian, A.J. et al. (1997) Expression of a mutant (Arg92Gln) human cardiac troponin T, known to cause hypertrophic cardiomyopathy, impairs adult cardiac myocyte contractility.
Circ Res. 81: 76-85. -
Dunnick, J.K. et al. (2004) Cardiac damage in rodents after exposure to bis(2-chloroethoxy)methane.
Toxicol Pathol. 32: 309-17. -
Herman, E.H. et al. (1999) Correlation between serum levels of cardiac troponin-T and the severity of the chronic cardiomyopathy induced by doxorubicin.
J Clin Oncol. 17: 2237-43. -
Zhang, J. et al. (2008) Isoproterenol-induced cardiotoxicity in sprague-dawley rats: correlation of reversible and irreversible myocardial injury with release of cardiac troponin T and roles of iNOS in myocardial injury.
Toxicol Pathol. 36: 277-8. -
Sweeney, H.L. et al. (1998) Functional analyses of troponin T mutations that cause hypertrophic cardiomyopathy: insights into disease pathogenesis and troponin function.
Proc Natl Acad Sci U S A. 95: 14406-10. -
Zhang, J. et al. (2006) Mechanisms and biomarkers of cardiovascular injury induced by phosphodiesterase inhibitor III SK&F 95654 in the spontaneously hypertensive rat.
Toxicol Pathol. 34: 152-63. -
Baharom, F. et al. (2016) Dendritic Cells and Monocytes with Distinct Inflammatory Responses Reside in Lung Mucosa of Healthy Humans.
J Immunol. 196 (11): 4498-509.
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