TIM-4 antibody | ROS-JH9
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Mouse anti Chicken TIM-4
- Product Type
- Monoclonal Antibody
|Mouse anti Chicken TIM-4 antibody, clone ROS-JH9, recognizes chicken TIM-4 (T-cell immunoglobulin mucin 4).
In mice, TIM-4 is largely expressed on antigen-presenting cells and involved in phagocytosis of apoptotic cells with, additionally, a regulatory role in T-cell responses. In chickens, the gene for TIM-4 codes for a minimum of five splice variants that vary between stains. Myeloid antigen-presenting cells and γδ T cells express chTIM4; in contrast to mammalian T cells which do not express TIM-4 (Hu et al. 2016).
Research conducted by Hu et al. using Mouse anti Chicken TIM-4 antibody, clone ROS-JH9, revealed that it can detect all isoforms of chicken TIM-4. Clone ROS-JH9 was found to stain bone marrow macrophages from J line birds, a cell population that was also positive for clone KUL01 MCA5770 (Hu et al. 2016). Further work characterized a distinct TIM-4 bearing set of phagocytes in the bursa and showed that approximately 50% of blood monocytes in chicken express detectable levels of TIM-4 (Hu et al. 2019).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Carrier Free
- Recombinant chicken TIM-4 extracellular domain (amino acids 1–209) fused to chicken IgFc region
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with Sp2/0‐Ag14 mouse myeloma cells
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Antibody Characterization Reference
Hu, T. et al. (2016) Functional annotation of the T-cell immunoglobulin mucin family in birds.
Immunology. 148 (3): 287-303.
Wu, Z. et al. (2016) Analysis of the function of IL-10 in chickens using specific neutralising antibodies and a sensitive capture ELISA.
Dev Comp Immunol. 63: 206-12.
Hu, T. et al. (2019) Characterization of Subpopulations of Chicken Mononuclear Phagocytes That Express TIM4 and CSF1R.
J Immunol. 202 (4): 1186-1199.
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