TIM-4 antibody | ROS-JH9
Mouse anti Chicken TIM-4
- Product Type
- Monoclonal Antibody
- Clone
- ROS-JH9
- Isotype
- IgG1
- Specificity
- TIM-4
Antibody Quality and Performance Guarantee
| Mouse anti Chicken TIM-4 antibody, clone ROS-JH9, recognizes chicken TIM-4 (T-cell immunoglobulin mucin 4). In mice, TIM-4 is largely expressed on antigen-presenting cells and involved in phagocytosis of apoptotic cells with, additionally, a regulatory role in T-cell responses. In chickens, the gene for TIM-4 codes for a minimum of five splice variants that vary between stains. Myeloid antigen-presenting cells and γδ T cells express chTIM4; in contrast to mammalian T cells which do not express TIM-4 (Hu et al. 2016). Research conducted by Hu et al. using Mouse anti Chicken TIM-4 antibody, clone ROS-JH9, revealed that it can detect all isoforms of chicken TIM-4. Clone ROS-JH9 was found to stain bone marrow macrophages from J line birds, a cell population that was also positive for clone KUL01 MCA5770 (Hu et al. 2016). Further work characterized a distinct TIM-4 bearing set of phagocytes in the bursa and showed that approximately 50% of blood monocytes in chicken express detectable levels of TIM-4 (Hu et al. 2019). |
- Target Species
- Chicken
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Carrier Free
- Yes
- Immunogen
- Recombinant chicken TIM-4 extracellular domain (amino acids 1–209) fused to chicken IgFc region
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with Sp2/0‐Ag14 mouse myeloma cells
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Antibody Characterization Reference
-
Hu, T. et al. (2016) Functional annotation of the T-cell immunoglobulin mucin family in birds.
Immunology. 148 (3): 287-303. -
Wu, Z. et al. (2016) Analysis of the function of IL-10 in chickens using specific neutralising antibodies and a sensitive capture ELISA.
Dev Comp Immunol. 63: 206-12.
Further Reading
-
Hu, T. et al. (2019) Characterization of Subpopulations of Chicken Mononuclear Phagocytes That Express TIM4 and CSF1R.
J Immunol. 202 (4): 1186-1199.
MCA6407GA
162045
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