Monocytes/Macrophages antibody | KUL01
Mouse anti Chicken Monocytes/Macrophages
- Product Type
- Monoclonal Antibody
- Clone
- KUL01
- Isotype
- IgG1
- Specificity
- Monocytes/Macrophages
Mouse anti Chicken Monocytes/Macrophages, clone KUL01 recognises chicken monocytes and macrophages. Mouse anti Chicken monocytes/ macrophages, clone KUL01 also detects interdigitating cells and activated microglia but does not recognise Bu1+ve B-cells or CD3 +ve T-lymphocytes. Mouse anti Chicken monocytes/ macrophages, clone KUL01 has subsequently been demonstrated to recognize chicken MRC1L-B, a homologue of the mammalian mannose receptor MRC1 (Staines et al. 2014). Five papalogous genes have been identified in the chicken genome, named MRC1L-A-E. Mouse anti Chicken monocytes / macrophages antibody only recognizes the MRC1L-B gene product.(Staines et al. 2014). |
- Target Species
- Chicken
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
- Buffer Solution
- Borate buffered saline.
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Chicken peripheral blood mononuclear leukocytes (PBML).
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Fusion Partners
- Spleen cells from Balb/c mice immunized with chicken PBML were fused with cells from the Sp2/0-Ag14 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | |||
Immunohistology - Frozen | |||
Immunohistology - Paraffin 1 |
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
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Source Reference
-
Mast, J. et al. (1998) Characterisation of chicken monocytes, macrophages and interdigitating cells by the monoclonal antibody KUL01.
Vet Immunol Immunopathol. 61 (2-4): 343-57.
References for Monocytes/Macrophages antibody
-
Wigley, P. et al. (2001) Salmonella enterica serovar Pullorum persists in splenic macrophages and in the reproductive tract during persistent, disease-free carriage in chickens.
Infect Immun. 69 (12): 7873-9. -
Balic, A. et al. (2014) Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
Development. 141: 3255-65. -
Garcia-Morales, C. et al. (2014) Production and characterisation of a monoclonal antibody that recognises the chicken CSF1 receptor and confirms that expression is restricted to macrophage-lineage cells.
Dev Comp Immunol. 42 (2): 278-85. -
Guabiraba, R. et al. (2017) Unveiling the participation of avian kinin ornithokinin and its receptors in the chicken inflammatory response.
Vet Immunol Immunopathol. 188: 34-47. -
Tomal, F. et al. (2023) Microbiota promotes recruitment and pro-inflammatory response of caecal macrophages during E. tenella infection.
Gut Pathog. 15 (1): 65. -
Alber, A. et al. (2019) Dose-dependent differential resistance of inbred chicken lines to avian pathogenic Escherichia coli challenge.
Avian Pathol. 48 (2): 157-67. -
Farsang, A. et al. (2019) Avian coronavirus infection induces mannose-binding lectin production in dendritic cell precursors of chicken lymphoid organs.
Acta Vet Hung. 67 (2): 183-96. -
Bryson, K.J. et al. (2020) Precision cut lung slices: a novel versatile tool to examine host-pathogen interaction in the chicken lung.
Vet Res. 51 (1): 2.
View The Latest Product References
-
Saint-Martin, V. et al. (2024) The gut microbiota and its metabolite butyrate shape metabolism and antiviral immunity along the gut-lung axis in the chicken.
Commun Biol. 7 (1): 1185.
- Synonyms
- MRC1L-B
- RRID
- AB_10841619
- UniProt
- M1XGZ4
MCA5770
150571 156457 166899If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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