MHC Class II Monomorphic antibody | 21-1A6
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Mouse anti Chicken MHC Class II (monomorphic) antibody, clone 21-1A6, recognizes a monomorphic determinant on the chicken B-L molecule, the chicken class II major histocompatability complex (MHC).
The level of B-L expression is reported to increase during the bursal phase of B cell differentiation (Veromaa, T. et al. 1988).
The level of B-L expression is reported to increase during the bursal phase of B cell differentiation (Veromaa, T. et al. 1988).
Product Details
- Target Species
- Chicken
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Chicken bursa cells
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of MHC Class II Monomorphic antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/50 | 1/100 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for MHC Class II Monomorphic antibody
-
Veromaa, T. et al. (1988) Expression of B-L and Bu-1 antigens in chickens bursectomized at 60 h of incubation.
Eur J Immunol. 18 (2): 225-30. -
Vainio, O. et al. (1988) Antigen-presenting cell-T cell interaction in the chicken is MHC class II antigen restricted.
J Immunol. 140 (9): 2864-8. -
Petkov, D.I. et al. (2009) Identification and characterization of two distinct bursal B-cell subpopulations following infectious bursal disease virus infection of White Leghorn chickens.
Avian Dis. 53 (3): 347-55. -
Silva, A.B. et al. (2008) Functional analysis of neuropeptides in avian thymocyte development.
Dev Comp Immunol. 32 (4): 410-20. -
Pavlova, S.P. et al. (2010) In vitro and in vivo characterization of glycoprotein C-deleted infectious laryngotracheitis virus.
J Gen Virol. 91 (Pt 4): 847-57. -
Wattrang, E. (2009) Phosphorothioate oligodeoxyribonucleotides induce in vitro proliferation of chicken B-cells.
Vet Immunol Immunopathol. 131 (3-4): 218-28. -
Kamble, N.M. et al. (2016) Interaction of a live attenuated Salmonella gallinarum vaccine candidate with chicken bone marrow-derived dendritic cells.
Avian Pathol. Jan 26:1-24. [Epub ahead of print] -
Jarosz, Ł. et al. (2016) Effects of feed supplementation with glycine chelate and iron sulfate on selected parameters of cell-mediated immune response in broiler chickens.
Research in Veterinary Science. Apr 30 [Epub ahead of print] -
Eren, U. et al. (2016) The several elements of intestinal innate immune system at the beginning of the life of broiler chicks.
Microsc Res Tech. Apr 26. [Epub ahead of print] -
Kamble, N.M. et al. (2016) Activation of chicken bone marrow-derived dendritic cells induced by a Salmonella enteritidis ghost vaccine candidate.
Poultry Science. May 3 [Epub ahead of print] -
Jarosz, Ł. et al. (2016) Effects of feed supplementation with glycine chelate and iron sulfate on selected parameters of cell-mediated immune response in broiler chickens.
Res Vet Sci. 107: 68-74. -
Shojadoost, B.et al. (2017) Interactions between lactobacilli and chicken macrophages induce antiviral responses against avian influenza virus
Research in Veterinary Science. Oct 31 [Epub ahead of print].
