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MHC Class II Monomorphic antibody | 21-1A6

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Mouse anti Chicken MHC Class II Monomorphic:FITC

Mouse anti Chicken MHC Class II Monomorphic

Product Type
Monoclonal Antibody
Clone
21-1A6
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2171F
Datasheet
F
SDS
0.1 mg loader

MCA2171GA
Datasheet
C F IP
SDS
0.1 mg loader

Mouse anti Chicken MHC Class II (monomorphic) antibody, clone 21-1A6, recognizes a monomorphic determinant on the chicken B-L molecule, the chicken class II major histocompatibility complex (MHC).

The level of B-L expression is reported to increase during the bursal phase of B cell differentiation (Veromaa et al. 1988).

Product Details

Target Species
Chicken
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Chicken bursa cells
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of MHC Class II Monomorphic antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for MHC Class II Monomorphic antibody

  1. Veromaa, T. et al. (1988) Expression of B-L and Bu-1 antigens in chickens bursectomized at 60 h of incubation.
    Eur J Immunol. 18 (2): 225-30.
  2. Vainio, O. et al. (1988) Antigen-presenting cell-T cell interaction in the chicken is MHC class II antigen restricted.
    J Immunol. 140 (9): 2864-8.
  3. Petkov, D.I. et al. (2009) Identification and characterization of two distinct bursal B-cell subpopulations following infectious bursal disease virus infection of White Leghorn chickens.
    Avian Dis. 53 (3): 347-55.
  4. Silva, A.B. et al. (2008) Functional analysis of neuropeptides in avian thymocyte development.
    Dev Comp Immunol. 32 (4): 410-20.
  5. Pavlova, S.P. et al. (2010) In vitro and in vivo characterization of glycoprotein C-deleted infectious laryngotracheitis virus.
    J Gen Virol. 91 (Pt 4): 847-57.
  6. Wattrang, E. (2009) Phosphorothioate oligodeoxyribonucleotides induce in vitro proliferation of chicken B-cells.
    Vet Immunol Immunopathol. 131 (3-4): 218-28.
  7. Kamble, N.M. et al. (2016) Interaction of a live attenuated Salmonella gallinarum vaccine candidate with chicken bone marrow-derived dendritic cells.
    Avian Pathol. 45 (2): 235-43.
  8. Jarosz, Ł. et al. et al. (2016) Effects of feed supplementation with glycine chelate and iron sulfate on selected parameters of cell-mediated immune response in broiler chickens.
    Res Vet Sci. 107: 68-74.
  9. Eren, U. et al. (2016) The several elements of intestinal innate immune system at the beginning of the life of broiler chicks.
    Microsc Res Tech. 79 (7): 604-14.
  10. Kamble, N.M. et al. (2016) Activation of chicken bone marrow-derived dendritic cells induced by a Salmonella Enteritidis ghost vaccine candidate.
    Poult Sci. 95 (10): 2274-80.
  11. Jarosz, Ł.S. et al. (2018) The effect of feed supplementation with a copper-glycine chelate and copper sulphate on selected humoral and cell-mediated immune parameters, plasma superoxide dismutase activity, ceruloplasmin and cytokine concentration in broiler chickens.
    J Anim Physiol Anim Nutr (Berl). 102 (1): e326-e336.
  12. Shojadoost, B. et al. (2019) Interactions between lactobacilli and chicken macrophages induce antiviral responses against avian influenza virus.
    Res Vet Sci. 125: 441-50.
  13. Yildiz, M. et al. (2019) Histological and immunohistochemical studies of the proximal caecum and caecal tonsils of quail (Coturnix coturnix japonica).
    Anat Histol Embryol. 48 (5): 476-85.
  14. Garrido, D. et al. (2018) The role of type I interferons (IFNs) in the regulation of chicken macrophage inflammatory response to bacterial challenge.
    Dev Comp Immunol. 86: 156-70.

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