MHC Class II Monomorphic antibody | 21-1A6

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Mouse anti Chicken MHC Class II Monomorphic:FITC

Mouse anti Chicken MHC Class II Monomorphic

Product Type: Monoclonal Antibody
Clone: 21-1A6
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2171F	F			0.1 mg
MCA2171GA	C F IP			0.1 mg

Mouse anti Chicken MHC Class II (monomorphic) antibody, clone 21-1A6, recognizes a monomorphic determinant on the chicken B-L molecule, the chicken class II major histocompatability complex (MHC).

The level of B-L expression is reported to increase during the bursal phase of B cell differentiation (Veromaa, T. et al. 1988).

Product Details

Target Species: Chicken
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Carrier Free: Yes
Immunogen: Chicken bursa cells
Approx. Protein Concentrations: IgG concentration 0.1 mg/ml
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunized Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch

More Information

Regulatory: For research purposes only

Applications of MHC Class II Monomorphic antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry		Neat
Flow Cytometry	1/50	1/100
Immunohistology - Frozen
Immunoprecipitation

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for MHC Class II Monomorphic antibody

Veromaa, T. et al. (1988) Expression of B-L and Bu-1 antigens in chickens bursectomized at 60 h of incubation.
Eur J Immunol. 18 (2): 225-30.
Vainio, O. et al. (1988) Antigen-presenting cell-T cell interaction in the chicken is MHC class II antigen restricted.
J Immunol. 140 (9): 2864-8.
Petkov, D.I. et al. (2009) Identification and characterization of two distinct bursal B-cell subpopulations following infectious bursal disease virus infection of White Leghorn chickens.
Avian Dis. 53 (3): 347-55.
Silva, A.B. et al. (2008) Functional analysis of neuropeptides in avian thymocyte development.
Dev Comp Immunol. 32 (4): 410-20.
Pavlova, S.P. et al. (2010) In vitro and in vivo characterization of glycoprotein C-deleted infectious laryngotracheitis virus.
J Gen Virol. 91 (Pt 4): 847-57.
Wattrang, E. (2009) Phosphorothioate oligodeoxyribonucleotides induce in vitro proliferation of chicken B-cells.
Vet Immunol Immunopathol. 131 (3-4): 218-28.
Kamble, N.M. et al. (2016) Interaction of a live attenuated Salmonella gallinarum vaccine candidate with chicken bone marrow-derived dendritic cells.
Avian Pathol. Jan 26:1-24. [Epub ahead of print]
Jarosz, Ł. et al. (2016) Effects of feed supplementation with glycine chelate and iron sulfate on selected parameters of cell-mediated immune response in broiler chickens.
Research in Veterinary Science. Apr 30 [Epub ahead of print]
Eren, U. et al. (2016) The several elements of intestinal innate immune system at the beginning of the life of broiler chicks.
Microsc Res Tech. Apr 26. [Epub ahead of print]
Kamble, N.M. et al. (2016) Activation of chicken bone marrow-derived dendritic cells induced by a Salmonella enteritidis ghost vaccine candidate.
Poultry Science. May 3 [Epub ahead of print]
Jarosz, Ł. et al. (2016) Effects of feed supplementation with glycine chelate and iron sulfate on selected parameters of cell-mediated immune response in broiler chickens.
Res Vet Sci. 107: 68-74.
Shojadoost, B.et al. (2017) Interactions between lactobacilli and chicken macrophages induce antiviral responses against avian influenza virus
Research in Veterinary Science. Oct 31 [Epub ahead of print].

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