IgY antibody | 4D12
Mouse anti Chicken IgY
- Product Type
- Monoclonal Antibody
- Clone
- 4D12
- Isotype
- IgG1
- Specificity
- IgY
Antibody Quality and Performance Guarantee
Mouse anti Chicken IgY antibody, clone 4D12 (MCA5681) used to label chicken B cells by flow cytometry.
Image caption:
B-cell phenotypes Leukocytes from the blood (B) and spleen (C) were stained for chB6 (AV20) and IgM (M1). Plots are gated for viable, single leukocytes. (D,E) Recognition of membrane-bound IgY. Spleen cells were stimulated for 6 days with CD40L and IL-10 to induce B-cell proliferation and class switch. Cells were stained with anti-chB6 (AV20) to address undifferentiated B cells and anti-IgY clone G1 (D) or anti-IgY clone 4D12 (E). Plots are gated for viable, single leukocytes. (F) Immediately after isolation (blue) or after stimulation with CD40L and IL-10 for 48 h (red), splenic leukocytes were analyzed for MHC class II (2G11), CD80 (AV82), CD25 (28–4) and CD57 (HNK-1) expression. Histograms were gated for viable, single chB6+ B cells.
From: Citation: Härtle S, Sutton K, Vervelde L and Dalgaard TS (2024)
Delineation of chicken immune markers in the era of omics and multicolor flow cytometry.
Front. Vet. Sci. 11:1385400.
doi: 10.3389/fvets.2024.1385400
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F Reset| Mouse anti Chicken IgY antibody, clone 4D12 recognizes chicken IgY. Clone 4D12 recognizes free IgY but does not bind IgY in it's membrane bound form and is not suitable the analysis on membrane bound IgY by flow cytometry (Härtle et al. 2024). |
- Target Species
- Chicken
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Purified chicken IgY heavy chain.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
5.0 | 300ng/ml |
| Flow Cytometry | |||
| Immunohistology - Frozen | |
1:20000 | |
| Western Blotting | |
0.5ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Chicken spleen
- Western Blotting
- MCA5681 detects a band of approximately 72kDa using purified chicken IgY under reducing conditions.
References for IgY antibody
-
Härtle, S. et al. (2024) Delineation of chicken immune markers in the era of omics and multicolor flow cytometry
Front Vet Sci. 23 May [Epub ahead of print].
- RRID
- AB_10674717
MCA5681
152994
310112R
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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