IFN Alpha antibody | UMCh3-IFNa
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Mouse anti Chicken Interferon Alpha
- Product Type
- Monoclonal Antibody
- Clone
- UMCh3-IFNa
- Isotype
- IgG1
- Specificity
- IFN Alpha
Antibody Quality and Performance Guarantee
| Mouse anti Chicken interferon alpha antibody, clone UMCh3-IFNa recognizes chicken Interferon alpha (chIFN-alpha). The classification of mammalian cytokines with antiviral activity, is well documented as being either Type I Interferon (IFNI) or Type II Interferon (IFNII). This is not the case for avian IFNs however, even though IFN was first detected in the chicken. There are similarities between chicken IFN and mammalian IFNI with conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes (Staeheli et al. 2001). Chicken IFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and that chicken IFN acts as a potent antiviral agent (Marcus et al. 1999). Mouse anti Chicken interferon alpha antibody, clone UMCh3-IFNa does not cross react with IFN beta or IFN gamma and does not neutralize chIFN-alpha. |
- Target Species
- Chicken
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Recombinant chicken Interferon alpha.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- MCA2412 detects a band of approximately 19 kDa.
References for IFN Alpha antibody
-
Sarmento, L. et al. (2008) Differential host gene expression in cells infected with highly pathogenic H5N1 avian influenza viruses.
Vet Immunol Immunopathol. 125 (3-4): 291-302. -
Prescott, M.A. et al. (2024) An alternative strategy to increasing influenza virus replication for vaccine production in chicken embryo fibroblast (DF-1) cells by inhibiting interferon alpha and beta using peptide-conjugated phosphorodiamidate morpholino oligomers.
J Med Microbiol. 73 (2) [Epub ahead of print].
Further Reading
-
Sick, C. et al. (1996) A family of genes coding for two serologically distinct chicken interferons.
J Biol Chem. 271 (13): 7635-9. -
Staeheli, P. et al. (2001) Cytokines of birds: conserved functions--a largely different look.
J Interferon Cytokine Res. 21 (12): 993-1010. -
Schwarz, H. et al. (2004) Synthesis of IFN-beta by virus-infected chicken embryo cells demonstrated with specific antisera and a new bioassay.
J Interferon Cytokine Res. 24 (3): 179-84.
- Synonyms
- Interferon Alpha
- RRID
- AB_2122226
- UniProt
- P42165
- Entrez Gene
- IFNA3
- GO Terms
- GO:0006952 defense response
- GO:0009615 response to virus
- GO:0005125 cytokine activity
- GO:0005126 cytokine receptor binding
- GO:0005615 extracellular space
MCA2412
156600
231111R
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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