IFN Alpha antibody | UMCh3-IFNa
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Mouse anti Chicken interferon alpha antibody, clone UMCh3-IFNa recognizes chicken Interferon alpha (chIFN-alpha).
The classification of mammalian cytokines with antiviral activity, is well documented as being either Type I Interferon (IFNI) or Type II Interferon (IFNII). This is not the case for avian IFNs however, even though IFN was first detected in the chicken.
A growing number of functional studies indicate similarities between chicken IFN and mammalian IFNI, revealing the conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes. Further research has shown that chIFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and that recombinant chicken IFN acts as a potent antiviral agent.
Mouse anti Chicken interferon alpha antibody, clone UMCh3-IFNa does not cross react with IFN beta or IFN gamma and does not neutralize chIFN-alpha.
The classification of mammalian cytokines with antiviral activity, is well documented as being either Type I Interferon (IFNI) or Type II Interferon (IFNII). This is not the case for avian IFNs however, even though IFN was first detected in the chicken.
A growing number of functional studies indicate similarities between chicken IFN and mammalian IFNI, revealing the conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes. Further research has shown that chIFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and that recombinant chicken IFN acts as a potent antiviral agent.
Mouse anti Chicken interferon alpha antibody, clone UMCh3-IFNa does not cross react with IFN beta or IFN gamma and does not neutralize chIFN-alpha.
Product Details
- Target Species
- Chicken
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Recombinant chicken Interferon alpha.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P42165
- Entrez Gene
- IFNA3
- GO Terms
- GO:0006952 defense response
- GO:0009615 response to virus
- GO:0005125 cytokine activity
- GO:0005126 cytokine receptor binding
- GO:0005615 extracellular space
- Regulatory
- For research purposes only
Applications of IFN Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- MCA2412 detects a band of approximately 19 kDa.
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Secondary Antibodies Available
Product Specific References
References for IFN Alpha antibody
-
Sarmento, L. et al. (2008) Differential host gene expression in cells infected with highly pathogenic H5N1 avian influenza viruses.
Vet Immunol Immunopathol. 125 (3-4): 291-302. -
Schwarz, H. et al. (2004) Synthesis of IFN-beta by virus-infected chicken embryo cells demonstrated with specific antisera and a new bioassay.
J Interferon Cytokine Res. 24 (3): 179-84.
Further Reading
-
Staeheli, P. et al. (2001) Cytokines of birds: conserved functions--a largely different look.
J Interferon Cytokine Res. 21 (12): 993-1010. -
Sick, C. et al. (1996) A family of genes coding for two serologically distinct chicken interferons.
J Biol Chem. 271 (13): 7635-9.
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