CD45 antibody | UM16-6
Mouse anti Chicken CD45 antibody, clone UM16-6 (MCA2413) used to evaluate CD45 expression on chicken cell populations by flow cytometry.
Image caption:
Identification of heterophils, monocytes, lymphocytes, thrombocytes and counting beads through singlet gating, FSC/SSC characteristics and using CD45-PerCp/Cy5.5, CD41/61-Fitc and KUL01-PE. From the initial dot-plot in A singlet gating (FSC-H vs FSC-A) was performed in B. From the singlet gate CD45 high and CD45 intermediate/SSC low events were gated in C. From the gate in C the SSC high events were gated in D and from this gate CD45 high/PE low events were gated as heterophils and CD45 high/PE high events were gated as counting beads in E. From the gate in C the KUL01 positive events were gated in F and from this gate the FSC high events were gated as monocytes in G. From the gate in C the CD41/61 high/CD45 intermediate events were gated as thrombocytes in H. In addition in H CD45 high/CD41/61 low were gated and these events were gated on FSC low/SSC low profile as lymphocytes in I. A representative chicken blood sample from an uninfected chicken on day –3 is shown. The antibody panel is described in Table 1.
From: Wattrang E, Eriksson H, Jinnerot T, Persson M, Bagge E, Söderlund R, Naghizadeh M, Dalgaard TS. Immune responses upon experimental Erysipelothrix rhusiopathiae infection of naïve and vaccinated chickens.
Vet Res. 2020 Sep 14;51(1):114.
doi: 10.1186/s13567-020-00830-9.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Chicken CD45 antibody, clone UM16-6 (MCA2413) used to evaluate CD45 expression on chicken cell populations by flow cytometry.
Image caption:
CD45 expression on A and B heterophils. Chickens were uninfected, or experimentally infected with ER on day 0, “naïve” and “vaccinated” groups. Blood samples were collected at the indicated days. In A expression is shown as median fluorescence intensity (MFI) in the heterophil gate, as defined in Additional file 2, and results are shown as group mean values ± 95% CI where non-overlapping CI indicate statistically significant differences. On days –3 and 15 n = 13/group, on days 1, 5 and 8 n = 7/group and on days 3 and 11 n = 6/group. In B CD45 expression on heterophils on day 1 after ER infection is shown as fluorescence intensity for one representative uninfected, “naïve” and “vaccinated” chicken, respectively. Histogram in B was compiled using the FlowJo™ software package. For details see "Materials and methods" section.
From: Wattrang E, Eriksson H, Jinnerot T, Persson M, Bagge E, Söderlund R, Naghizadeh M, Dalgaard TS. Immune responses upon experimental Erysipelothrix rhusiopathiae infection of naïve and vaccinated chickens.
Vet Res. 2020 Sep 14;51(1):114.
doi: 10.1186/s13567-020-00830-9.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Chicken CD45 antibody, clone UM16-6 (MCA2413) used to evaluate CD45 expression on chicken cell populations by flow cytometry.
Image caption:
CD45 expression on C and D monocytes. Chickens were uninfected, or experimentally infected with ER on day 0, “naïve” and “vaccinated” groups. Blood samples were collected at the indicated days. In C expression is shown as median fluorescence intensity (MFI) monocyte gate, as defined in Additional file 2, and results are shown as group mean values ± 95% CI where non-overlapping CI indicate statistically significant differences. On days –3 and 15 n = 13/group, on days 1, 5 and 8 n = 7/group and on days 3 and 11 n = 6/group. In B CD45 expression on heterophils on day 1 after ER infection is shown as fluorescence intensity for one representative uninfected, “naïve” and “vaccinated” chicken, respectively. In D CD45 expression on monocytes on day 3 after ER infection is shown as fluorescence intensity for one representative uninfected, “naïve” and “vaccinated” chicken, respectively. Histogram D was compiled using the FlowJo™ software package. For details see "Materials and methods" section.
From: Wattrang E, Eriksson H, Jinnerot T, Persson M, Bagge E, Söderlund R, Naghizadeh M, Dalgaard TS. Immune responses upon experimental Erysipelothrix rhusiopathiae infection of naïve and vaccinated chickens.
Vet Res. 2020 Sep 14;51(1):114.
doi: 10.1186/s13567-020-00830-9.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Chicken CD45 antibody, clone UM16-6 (MCA2413GA) used to evaluate CD45 expression on chicken lung cells by flow cytometry.
Image caption:
Phenotyping of lung cells infected with APEC by flow cytometry. Gradient purified lung cells were isolated and subjected to flow cytometric analysis to phenotype APECpos cells against a panel of cell surface markers. (A) Representative graphs of APEC O1-GFP inoculated birds gated for single, live cells. APEC O1-GFPpos cells were gated against CSF1R-transgene in the top left graph visualising the APEC O1-GFPpos cells amongst the CSF1R-tgneg, CSF1R-tglow and CSF1R-tghigh cells. The horizontal gate divides the CSF1R-tgneg and CSF1R-tglow cells. Further phenotypic analysis showed that APEC O1-GFPpos cells are CD45pos CD11pos GRL1pos GRL2pos, and CD3neg chB6neg K1neg/low. A positive and negative MRC1L-B and MHC II cell population was detected within the APEC O1-GFPpos cells.
From: Alber A, Morris KM, Bryson KJ, Sutton KM, Monson MS, Chintoan-Uta C, Borowska D, Lamont SJ, Schouler C, Kaiser P, Stevens MP, Vervelde L.
Avian Pathogenic Escherichia coli (APEC) Strain-Dependent Immunomodulation of Respiratory Granulocytes and Mononuclear Phagocytes in CSF1R-Reporter Transgenic Chickens.
Front Immunol. 2020 Jan 10;10:3055.
doi: 10.3389/fimmu.2019.03055.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Chicken CD45 antibody, clone UM16-6 (MCA2413GA) used to evaluate CD45 expression on chicken lung cells by flow cytometry.
Image caption:
Phenotyping of lung cells infected with APEC by flow cytometry. Gradient purified lung cells were isolated and subjected to flow cytometric analysis to phenotype APECpos cells against a panel of cell surface markers. (C) Representative graphs of APEC O2-GFP inoculated birds gated for single live cells and plotted identically to the APEC O1-GFP inoculated birds without the GRL1 and GRL2 antibody staining which was only performed with APEC O1-GFP inoculated birds. APEC O2-GFPpos cells were CD45pos CD11pos, and CD3neg chB6neg K1. Again, a positive and negative MRC1L-B and MHC II cell population was detected within the APEC O2-GFPpos cells.
From: Alber A, Morris KM, Bryson KJ, Sutton KM, Monson MS, Chintoan-Uta C, Borowska D, Lamont SJ, Schouler C, Kaiser P, Stevens MP, Vervelde L.
Avian Pathogenic Escherichia coli (APEC) Strain-Dependent Immunomodulation of Respiratory Granulocytes and Mononuclear Phagocytes in CSF1R-Reporter Transgenic Chickens.
Front Immunol. 2020 Jan 10;10:3055.
doi: 10.3389/fimmu.2019.03055.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Chicken CD45
- Product Type
- Monoclonal Antibody
- Clone
- UM16-6
- Isotype
- IgG2a
- Specificity
- CD45
Antibody Quality and Performance Guarantee
| Mouse anti Chicken CD45 antibody, clone UM16-6 recognizes chicken CD45, also known as leucocyte common antigen. CD45 is a heavily-glycosylated transmembrane protein tyrosine phosphatase (PTPase) expressed by all nucleated cells of haematopoietic origin. Variation in the expression of a particular CD45 isoform, is regulated during the haematopoietic development of the different cell lineages. CD45 is essential for antigen-induced signal transduction through the antigen receptor and as with other PTPase family members, acts in balance with protein tyrosine kinases, causing the dephosphorylation of negative regulatory tyrosine sites. De-phosphorylation by CD45, is required for the activation of the src-family kinases p56lck and p59fyn. Chicken CD45 has an additional cysteine residue near the transmembrane region compared to human and shark CD45 (Okumura et al. 1996). The overall domain structure between mammalian and chicken CD45 appears to be conserved, but the sequence homology between the extracellular regions is very low. |
- Target Species
- Chicken
- Species Cross-Reactivity
-
Target Species Cross Reactivity Turkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- COS cells transfected with CD45 gene.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/100 | 1/200 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Histology Positive Control Tissue
- Chicken lung
References for CD45 antibody
-
Reddy SK et al. (2008) The BAFF-Interacting receptors of chickens.
Dev Comp Immunol. 32 (9): 1076-87. -
Pavlova, S. et al. (2010) In vitro and in vivo characterization of glycoprotein C-deleted infectious laryngotracheitis virus.
J Gen Virol. 91:847-57. -
Harvanová, D. et al. (2014) Isolation, cultivation and characterisation of pigeon osteoblasts seeded on xenogeneic demineralised cancellous bone scaffold for bone grafting.
Vet Res Commun. 38 (3): 221-8. -
Ulrich-Lynge SL et al. (2015) The consequence of low mannose-binding lectin plasma concentration in relation to susceptibility to Salmonella infantis in chickens.
Vet Immunol Immunopathol. 163 (1-2): 23-32. -
Wattrang, E. et al. (2015) CD107a as a marker of activation in chicken cytotoxic T cells.
J Immunol Methods. 419: 35-47. -
Czerwiński, J. et al. (2015) The use of genetically modified Roundup Ready soyabean meal and genetically modified MON 810 maize in broiler chicken diets.
Part 1. Effects on performance and blood lymphocyte subpopulations
J Anim Feed Sci 24: 134-43. -
Eren, U. et al. (2016) The several elements of intestinal innate immune system at the beginning of the life of broiler chicks.
Microsc Res Tech. 79 (7): 604-14. -
Röhe, I. et al. (2017) Effect of feeding soybean meal and differently processed peas on the gut mucosal immune system of broilers
Poultry Science. Feb 23 [Epub ahead of print]
View The Latest Product References
-
Kjærup, R.B. et al. (2017) Comparison of growth performance and immune parameters of three commercial chicken lines used in organic production.
Vet Immunol Immunopathol. 187: 69-79. -
Fenzl, L. et al. (2017) γδ T cells represent a major spontaneously cytotoxic cell population in the chicken.
Dev Comp Immunol. 73: 175-83. -
Larsen, F.T. et al. (2019) Immunoprofiling of peripheral blood from infectious bronchitis virus vaccinated MHC-B chicken lines - Monocyte MHC-II expression as a potential correlate of protection.
Dev Comp Immunol. 96: 93-102. -
Naghizadeh, M. et al. (2019) Rapid whole blood assay using flow cytometry for measuring phagocytic activity of chicken leukocytes.
Vet Immunol Immunopathol. 207: 53-61. -
Wattrang, E. et al. (2020) Immune responses upon experimental Erysipelothrix rhusiopathiae infection of naïve and vaccinated chickens.
Vet Res. 51 (1): 114. -
Alber, A. et al. (2019) Avian Pathogenic Escherichia coli (APEC) Strain-Dependent Immunomodulation of Respiratory Granulocytes and Mononuclear Phagocytes in CSF1R-Reporter Transgenic Chickens.
Front Immunol. 10: 3055.
Further Reading
-
Okumura, M. et al. (1996) Comparison of CD45 extracellular domain sequences from divergent vertebrate species suggests the conservation of three fibronectin type III domains.
J Immunol. 157 (4): 1569-75. -
Jung, E.J. et al. (1997) Phosphorylation of chicken protein tyrosine phosphatase 1 by casein kinase II in vitro.
Experimental and Molecular Medicine 29(4): 229-33. -
Symons, A. et al. (1999) Domain organization of the extracellular region of CD45.
Protein Eng. 12 (10): 885-92.
- Synonyms
- Leucocyte Common Antigen
MCA2413GA
150839
161355
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