CD45 antibody | UM16-6
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Mouse anti Chicken CD45
- Product Type
- Monoclonal Antibody
- Clone
- UM16-6
- Isotype
- IgG2a
- Specificity
- CD45
Antibody Quality and Performance Guarantee
| Mouse anti Chicken CD45 antibody, clone UM16-6 recognizes chicken CD45, also known as leucocyte common antigen. CD45 is a heavily-glycosylated transmembrane protein tyrosine phosphatase (PTPase) expressed by all nucleated cells of haematopoietic origin. Variation in the expression of a particular CD45 isoform, is regulated during the haematopoietic development of the different cell lineages. CD45 is essential for antigen-induced signal transduction through the antigen receptor and as with other PTPase family members, acts in balance with protein tyrosine kinases, causing the dephosphorylation of negative regulatory tyrosine sites. De-phosphorylation by CD45, is required for the activation of the src-family kinases p56lck and p59fyn. Chicken CD45 has an additional cysteine residue near the transmembrane region compared to human and shark CD45 (Okumura et al. 1996). The overall domain structure between mammalian and chicken CD45 appears to be conserved, but the sequence homology between the extracellular regions is very low. |
- Target Species
- Chicken
- Species Cross-Reactivity
-
Target Species Cross Reactivity Turkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- COS cells transfected with CD45 gene.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/100 | 1/200 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Histology Positive Control Tissue
- Chicken lung
References for CD45 antibody
-
Reddy SK et al. (2008) The BAFF-Interacting receptors of chickens.
Dev Comp Immunol. 32 (9): 1076-87. -
Pavlova, S. et al. (2010) In vitro and in vivo characterization of glycoprotein C-deleted infectious laryngotracheitis virus.
J Gen Virol. 91:847-57. -
Harvanová, D. et al. (2014) Isolation, cultivation and characterisation of pigeon osteoblasts seeded on xenogeneic demineralised cancellous bone scaffold for bone grafting.
Vet Res Commun. 38 (3): 221-8. -
Wattrang, E. et al. (2015) CD107a as a marker of activation in chicken cytotoxic T cells.
J Immunol Methods. 419: 35-47. -
Ulrich-Lynge SL et al. (2015) The consequence of low mannose-binding lectin plasma concentration in relation to susceptibility to Salmonella infantis in chickens.
Vet Immunol Immunopathol. 163 (1-2): 23-32. -
Eren, U. et al. (2016) The several elements of intestinal innate immune system at the beginning of the life of broiler chicks.
Microsc Res Tech. 79 (7): 604-14. -
Röhe, I. et al. (2017) Effect of feeding soybean meal and differently processed peas on the gut mucosal immune system of broilers
Poultry Science. Feb 23 [Epub ahead of print] -
Czerwiński, J. et al. (2015) The use of genetically modified Roundup Ready soyabean meal and genetically modified MON 810 maize in broiler chicken diets.
Part 1. Effects on performance and blood lymphocyte subpopulations
J Anim Feed Sci 24: 134-43.
View The Latest Product References
-
Kjærup, R.B. et al. (2017) Comparison of growth performance and immune parameters of three commercial chicken lines used in organic production.
Vet Immunol Immunopathol. 187: 69-79. -
Fenzl, L. et al. (2017) γδ T cells represent a major spontaneously cytotoxic cell population in the chicken.
Dev Comp Immunol. 73: 175-83. -
Larsen, F.T. et al. (2019) Immunoprofiling of peripheral blood from infectious bronchitis virus vaccinated MHC-B chicken lines - Monocyte MHC-II expression as a potential correlate of protection.
Dev Comp Immunol. 96: 93-102. -
Naghizadeh, M. et al. (2019) Rapid whole blood assay using flow cytometry for measuring phagocytic activity of chicken leukocytes.
Vet Immunol Immunopathol. 207: 53-61.
Further Reading
-
Okumura, M. et al. (1996) Comparison of CD45 extracellular domain sequences from divergent vertebrate species suggests the conservation of three fibronectin type III domains.
J Immunol. 157 (4): 1569-75. -
Jung, E.J. et al. (1997) Phosphorylation of chicken protein tyrosine phosphatase 1 by casein kinase II in vitro.
Experimental and Molecular Medicine 29(4): 229-33. -
Symons, A. et al. (1999) Domain organization of the extracellular region of CD45.
Protein Eng. 12 (10): 885-92.
- Synonyms
- Leucocyte Common Antigen
MCA2413GA
150839
161355
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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