Salmonella enteritidis LPS antibody | 10D9H (E91)

Mouse anti Salmonella enteritidis LPS Group D 0-12 Antigen

Product Type
Monoclonal Antibody
Clone
10D9H (E91)
Isotype
IgG1
Specificity
Salmonella enteritidis LPS
Region
GROUP D 0-12 ANTIGEN

Product Code Applications Pack Size List Price Your Price Qty
8209-5349
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.2 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Salmonella enteritidis antibody, clone 10D9H recognizes a common antigenic determinant that is found in the LPS of Salmonella enteriditis (Group D), Salmonella typhimurium (Group B) and Salmonella paratyphi (Group A) . This antibody is thought to recognise the '0-12' antigen [2-D-mannose (1-4) I- ramnose (1-3)-D galactose] of salmonella LPS. A similar sequence is found in Group E, which may account for the weak cross reactivity observed with bacteria of this group.

Target Species
Bacterial
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Heat inactivated cells from Salmonella (LPS groups A, B & D).
Approx. Protein Concentrations
1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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Source Reference

  1. Merkulova, T.I. et al. (1995) Monoclonal antibodies directed against unique and common determinants on the lipopolysaccharide molecule of Salmonella serogroups A, B, and D.
    Hybridoma. 14 (6): 557-62.

Antibody Characterization Reference

  1. Authors not listed. (1995) MAb SD:10D9H, anti-Salmonella LPS
    Hybridoma. April 2009, 14(6): 625-625

RRID
AB_619298
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