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Escherichia coli Labile Toxin A Chain antibody | 20/480

Mouse anti Escherichia coli Labile Toxin A Chain

Product Type
Monoclonal Antibody
Clone
20/480
Isotype
IgG1
Specificity
Escherichia coli Labile Toxin A Chain

Product Code Applications Pack Size List Price Your Price Qty
4330-1088
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E IF WB * 0.2 mg loader
List Price Your Price
loader

Mouse antiEscherichia coliLabile Toxin A Chain antibody, clone 20/480 recognizes the A chain of Escherichia coli heat-labile enterotoxin, a pathogenic peptide that activates intracellular adenyl cyclase.

This antibody is reactive with isolates fixed by phenol, formalin and boiling.

Target Species
Bacterial
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Escherichia coli heat-labile enterotoxin.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Western Blotting 1
  1. 1This antibody reacts with Cholera toxin A chain under reducing conditions in Western blotting.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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References for Escherichia coli Labile Toxin A Chain antibody

  1. Koprowski, H., 2nd et al. (2000) Attenuated Shigella flexneri 2a vaccine strain CVD 1204 expressing colonization factor antigen I and mutant heat-labile enterotoxin of enterotoxigenic Escherichia coli
    Infect Immun 68: 4884

Western Blotting

RRID
AB_618972
UniProt
P43530
GO Terms
GO:0003824 catalytic activity
GO:0005576 extracellular region
GO:0009405 pathogenesis

4330-1088

154853

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