Escherichia coli J5 LPS antibody | GNE13-337.5 (2D7/1)
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Mouse anti Escherichia coli J5 LPS
- Product Type
- Monoclonal Antibody
- Clone
- GNE13-337.5 (2D7/1)
- Isotype
- IgG2b
- Specificity
- Escherichia coli J5 LPS
Antibody Quality and Performance Guarantee
| Mouse anti Escherichia coli J5 LPS antibody, clone 2D7/1 recognizes E. coli J5 LPS. The J5 mutant of E. coli lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The J5 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella). The clone 2D7/1 has also been found to react with K. pneumoniae, S. sonnei and S. typhimurium. |
- Target Species
- Bacterial
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- E. coli J5 cells.
- Approx. Protein Concentrations
- 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
References for Escherichia coli J5 LPS antibody
-
Yao, Z. et al. (2012) Regulation of cell size in response to nutrient availability by fatty acid biosynthesis in Escherichia coli.
Proc Natl Acad Sci U S A. 109 (38): E2561-8.
- RRID
- AB_619003
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