Escherichia coli J5 LPS antibody | GNE13-337.5 (2D7/1)

Mouse anti Escherichia coli J5 LPS

Product Type
Monoclonal Antibody
Clone
GNE13-337.5 (2D7/1)
Isotype
IgG2b
Specificity
Escherichia coli J5 LPS

Product Code Applications Pack Size List Price Your Price Qty
4329-5004
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Escherichia coli J5 LPS antibody, clone 2D7/1 recognizes E. coli J5 LPS.

The J5 mutant of E. coli lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The J5 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).

The clone 2D7/1 has also been found to react with K. pneumoniae, S. sonnei and S. typhimurium.

Target Species
Bacterial
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
E. coli J5 cells.
Approx. Protein Concentrations
0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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References for Escherichia coli J5 LPS antibody

  1. Yao, Z. et al. (2012) Regulation of cell size in response to nutrient availability by fatty acid biosynthesis in Escherichia coli.
    Proc Natl Acad Sci U S A. 109 (38): E2561-8.

RRID
AB_619003
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