Escherichia coli antibody | 1011
Mouse anti Escherichia coli antibody, clone 1011 (OBT0749) used as a capture antibody in an E. coli biosensor.
Image caption:
(A) Testing the binding abilities of different polyclonal Abs with an in-house ELISA test with different amounts of intact E. coli cells as antigen. Red: Bio-Rad 4329-4906, Blue: Thermo Fisher PA1-7213, Green: Thermo Fisher PA1-73032. (B) The binding ability of a monoclonal Ab (Bio-Rad OBT0749) with an in-house ELISA test with different amounts of intact E. coli cells as antigen. For detection, biotinylated polyclonal Ab (Bio-Rad 4329-4906) was used. The inset shows the schematic drawing of the assay.
From: Farkas, E.; Tarr, R.; Gerecsei, T.; Saftics, A.; Kovács, K.D.; Stercz, B.; Domokos, J.; Peter, B.; Kurunczi, S.; Szekacs, I.; Bonyár, A.; Bányai, A.; Fürjes, P.; Ruszkai-Szaniszló, S.; Varga, M.; Szabó, B.; Ostorházi, E.; Szabó, D.; Horvath, R.
Development and In-Depth Characterization of Bacteria Repellent and Bacteria Adhesive Antibody-Coated Surfaces Using Optical Waveguide Biosensing.
Biosensors 2022, 12, 56.
doi: 10.3390/bios12020056
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Escherichia coli antibody, clone 1011 (OBT0749) used as a capture antibody in an E. coli biosensor.
Image caption:
Typical OWLS kinetic curves of Ab deposition and their corresponding fits are shown. (A) Polyclonal Ab (Bio-Rad rabbit 4329-4906 on protein A surface). (B) Monoclonal Ab (Bio-Rad mouse OBT0749 on protein A surface). The smaller adsorbed surface mass density and slower adsorption are clearly visible in the case of the monoclonal Ab.
From: Farkas, E.; Tarr, R.; Gerecsei, T.; Saftics, A.; Kovács, K.D.; Stercz, B.; Domokos, J.; Peter, B.; Kurunczi, S.; Szekacs, I.; Bonyár, A.; Bányai, A.; Fürjes, P.; Ruszkai-Szaniszló, S.; Varga, M.; Szabó, B.; Ostorházi, E.; Szabó, D.; Horvath, R.
Development and In-Depth Characterization of Bacteria Repellent and Bacteria Adhesive Antibody-Coated Surfaces Using Optical Waveguide Biosensing.
Biosensors 2022, 12, 56.
doi: 10.3390/bios12020056
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Escherichia coli antibody, clone 1011 (OBT0749) used as a capture antibody in an E. coli biosensor.
Image caption:
Bacterial adsorption on the Ab-coated sensor surfaces and the detection limit of OWLS. (A) Sensogram of a complete experiment, including the in situ coating procedures and subsequent bacteria adsorption for protein A-based immobilization and PAcrAM-P blocking. (B) E. coli adsorption on polyclonal Ab-coated surfaces using PAcrAM-P blocking; a 109 cells/mL concentration was employed. The signals with statistics are clearly distinguishable from the relevant control signal (full layer without Ab). (C) OWLS signal for a series of bacterial concentrations using protein A-based immobilization with PAcrAM-P blocking.
From: Farkas, E.; Tarr, R.; Gerecsei, T.; Saftics, A.; Kovács, K.D.; Stercz, B.; Domokos, J.; Peter, B.; Kurunczi, S.; Szekacs, I.; Bonyár, A.; Bányai, A.; Fürjes, P.; Ruszkai-Szaniszló, S.; Varga, M.; Szabó, B.; Ostorházi, E.; Szabó, D.; Horvath, R.
Development and In-Depth Characterization of Bacteria Repellent and Bacteria Adhesive Antibody-Coated Surfaces Using Optical Waveguide Biosensing.
Biosensors 2022, 12, 56.
doi: 10.3390/bios12020056
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Escherichia coli
- Product Type
- Monoclonal Antibody
- Clone
- 1011
- Isotype
- IgG1
- Specificity
- Escherichia coli
Antibody Quality and Performance Guarantee
| Mouse anti Escherichia coli antibody, clone 1011 recognizes Escherichia coli. (E.coli.) andreacts with a number of E.coli. serotypes including K12, O18, O44, O112 and O125. Mouse anti Escherichia coli antibody, clone 1011 does not cross-react with other members of the Enterobacteriaceae. |
- Target Species
- Bacterial
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- A mixture of E. coli serotypes.
- Approx. Protein Concentrations
- 0.1mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/20 | 1/200 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for Escherichia coli antibody
-
Kim, M.Y. et al. (2016) Oral immunisation of mice with transgenic rice calli expressing cholera toxin B subunit fused to consensus dengue cEDIII antigen induces antibodies to all four dengue serotypes.
Plant Mol Biol. 92 (3): 347-56. -
Farkas, E. et al. (2022) Development and In-Depth Characterization of Bacteria Repellent and Bacteria Adhesive Antibody-Coated Surfaces Using Optical Waveguide Biosensing
Biosensors. 12 (2): 56.
- RRID
- AB_609632
OBT0749
150904
153180
160038
163946
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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