Clostridium difficile Toxin B antibody | 5158

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Mouse anti Clostridium difficile Toxin B

Product Type
Monoclonal Antibody
Clone
5158
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA4738 E 0.1 mg
Mouse anti Clostridium difficile Toxin B antibody, clone 5158 recognizes Clostridium difficile toxin B. No reaction is observed with toxin A. Clostridium difficile is a gram-positive motile bacterium which is the leading cause of diarrhoea in developed countries. The incidence of disease in humans varies greatly with age, spore density and the administration of antibiotics.

Toxin B (TcdB) is a large exotoxin. Its role in disease is less well understood than that of toxin A, though it seems clear that TcdB contributes to disease. Both toxins modify the Ras superfamily of small GTPases via glycosylation, inactivating them and leading to the disruption of vital signaling pathways in the cell.

Product Details

Target Species
Bacterial
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 0.1mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P18177 Related reagents
GO Terms
GO:0009405 pathogenesis
GO:0046527 glucosyltransferase activity
Regulatory
For research purposes only

Applications of Clostridium difficile Toxin B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/20 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Product Specific References

Further Reading

  1. Voth, D.E. & Ballard, J.D. (2005) Clostridium difficile toxins: mechanism of action and role in disease.
    Clin Microbiol Rev. 18 (2): 247-63.