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Browsing Modes for Business Users
-
Create mode – the default mode when you create a requisition and PunchOut to Bio-Rad. You can create and edit multiple shopping carts
-
Edit mode – allows you to edit or modify an existing requisition (prior to submitting). You will be able to modify only the cart that you have PunchedOut to, and will not have access to any other carts
-
Inspect mode – when you PunchOut to Bio-Rad from a previously created requisition but without initiating an Edit session, you will be in this mode. You cannot modify any Cart contents
Browsing Modes for Business Users
-
Create mode – the default mode when you create a requisition and PunchOut to Bio-Rad. You can create and edit multiple shopping carts
-
Edit mode – allows you to edit or modify an existing requisition (prior to submitting). You will be able to modify only the cart that you have PunchedOut to, and will not have access to any other carts
-
Inspect mode – when you PunchOut to Bio-Rad from a previously created requisition but without initiating an Edit session, you will be in this mode. You cannot modify any Cart contents
Webinar: Fluorescence and Compensation in Flow Cytometry
Why Fluorophores need Compensating and How to Avoid Unnecessary Compensation
Fluorophores accept light at a specific wavelength and re-emit it at a higher wavelength, giving each fluorophore an excitation and emission wavelength. Although there is a peak or maximal emission the range is often several hundred nanometers. This can cause problems where unwanted light enters several detectors giving false positives. Compensation is required to remove this unwanted fluorescence. Careful choice of fluorophores can reduce the amount of compensation needed.
The webinar is recommended to novices and researchers with limited experience of flow cytometry.
The webinar covered:
- Basic fluorescence principles
- What is compensation and why you need to apply it
- Compensation controls
- How to avoid compensation in small panels
- Common rules to help build more complex panels
Presented by: Dr Mike Blundell, Product Manager at Bio-Rad Laboratories
Speaker Biography:
Mike Blundell graduated from the University of Edinburgh with a B.Sc. in Immunology. He then moved to University College London where he joined the group of Prof. Adrian Thrasher and obtained his Ph.D. His thesis research was focussed on the primary immunodeficiency Wiskott Aldrich Syndrome. He investigated novel activating mutations and developed lentiviral vectors for use in gene therapy treatments, some of which have now been used in clinical trials. Mike contributed to 50 peer reviewed papers and has now left academia to become a product manager for Flow Cytometry.
Popular Resources
Blog: Sample Preparation in Flow Cytometry — Tips & Tricks
In this blog, we provide tips and advice on generating a high-quality sample for flow cytometry.
Quick Guide: How to Implement an Effective Flow Cytometry Gating Strategy
Download this quick guide to learn how to develop effective flow cytometry gating strategies, avoid common pitfalls such as false positives and missed rare populations, and generate reliable, reproducible data through a systematic approach to panel design and analysis.