Tubulin Beta 3 Antibody | TU-20

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  • Mouse anti Tubulin Beta 3:FITC
  • Mouse anti Tubulin Beta 3
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    TU-20
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2047FIFdatasheet pdfdatasheet pdf0.1 mg
    MCA2047F
    MCA2047C, IF, P *, WB*datasheet pdfdatasheet pdf0.1 mg
    MCA2047
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Tubulin beta 3 antibody, clone TU-20 recognizes class III beta-tubulin which is reported to be restricted to neuronal tissue.

      Mouse anti Tubulin beta 3 antibody, clone TU-20 has been used to investigate tumors of neuronal origin including neuroblastoma and ganglioneuroma. Class III beta tubulin has not been detected in tumors of non neuronal origin.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Baboonyes
      Ratyes
      Hamsteryes
      Pigyes
      Bovineyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by DEAE chromatography
      Purified IgG prepared from ascites by DEAE chromatography
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
    • Immunogen
      Synthetic peptide, ESESQGPK, corresponding to amino acids 441-448 of human class III beta tubulin coupled to Keyhole Limpet Hemocyanin (KLH).

      This sequence is conserved across all species.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      cytoplasm
      protein polymerization
      axon guidance
      'de novo' posttranslational protein folding
      GTP binding
      microtubule-based movement
      structural molecule activity
      GTPase activity
      microtubule
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence1/101/50
    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
      Immunohistology - Frozen
      Immunohistology - Paraffin(1)520 ug/ml
      Western Blotting(2)110 ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
      (2)
      TU-20 recognizes tubulin beta 3 under reducing conditions

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Neuronal tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Tubulin Beta 3 Antibody Formats

    Formats Clone Applications Sizes available
    Tubulin Beta 3 Antibody : FITC TU-20 IF 0.1 mg
    Tubulin Beta 3 Antibody : Purified TU-20 C, IF, P *, WB* 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Neuronal tissue

              References

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