Talin-1 Antibody | 97H6

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Talin-1 Antibody | 97H6 gallery image 1

Murine embryonic fibroblast stained with Mouse anti talin 1 antibody, clone 97H6 (MCA4770) and Mouse anti talin-2 antibody, clone 68E7 by immunofluorescence demonstrating partial overlapping expression. Originator image

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Talin-1 Antibody | 97H6 gallery image 2

Subcellular localisation o f talin1 and talin2 in cultures cells. Three cells types mouse NIH3T3, undifferentiated C2C12 cells and mouse embryo fibroblasts were fixed and stained for either talin1 using Mouse anti talin-1 antibody, clone 97H6 (MCA4770) (left column) or talin2 using Mouse anti Talin-2 antibody, clone 68E7 (right column), both in green and actin in red. Cells were fixed and permeabilised using methanol. Originator image

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  • Talin-1 Antibody | 97H6 thumbnail image 1
  • Talin-1 Antibody | 97H6 thumbnail image 2
  • Mouse anti Human Talin-1
  • Mouse anti Human Talin-1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    97H6
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4770GAIF*, IP, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA4770GA
    MCA4770IF*, IP, P *, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA4770
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Talin-1 antibody, clone 97H6 recognizes an epitope within the region of amino acids 482-655 of humanTalin-1, a high molecular weight cytoskeletal protein, linking vinculin to the integrins, thereby linking the cytoskeleton to the extracellular matrix. It is specifically found in regions of cell-substratum contact and in lymphocytes at cell-cell contact points. Talin-1 plays a role in the assembly of actin filaments and in the migration of cells such as osteoclasts and fibroblasts.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunoprecipitation
      Western Blotting
      Immunofluorescence(1)
      Immunohistology - Paraffin(2)1/25
      1/100Pack Size: 0.2 mg
      1/200Pack Size: 0.1 mg
      (1)
      Methanol treatment is required prior to antibody staining
      (2)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunoprecipitation
      Western Blotting
      Immunofluorescence(1)
      Immunohistology - Paraffin(2)1/25
      1/100Pack Size: 0.2 mg
      1/200Pack Size: 0.1 mg
      (1)
      Methanol treatment is required prior to antibody staining
      (2)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Tonsil
    • Histology Positive Control Tissue
      Tonsil
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Talin-1 Antibody Formats

    Formats Clone Applications Sizes available
    Talin-1 Antibody : Purified 97H6 IF*, IP, P *, WB 0.1 mg | 0.2 mg
    • Copyright © 2016 Bio-Rad

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      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Tonsil
              Tonsil

              Source Reference

              References

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