SQSTM1 Antibody | 2C11

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SQSTM1 Antibody | 2C11 gallery image 1

Immunofluorescence showing SQSTM1 in HeLa cells using Mouse anti Human SQSTM1:Azide Free (MCA4265Z)

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SQSTM1 Antibody | 2C11 gallery image 2

Western blot analysis of Hela whole cell lysate probed with Mouse anti Human SQSTM1 antibody (MCA4265Z) followed by HRP conjugated Goat anti Mouse IgG, visualized using chemiluminescence

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  • SQSTM1 Antibody | 2C11 thumbnail image 1
  • SQSTM1 Antibody | 2C11 thumbnail image 2
  • Mouse anti Human SQSTM1:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    2C11
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4265ZIF, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA4265Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human SQSTM1 antibody, clone 2C11 recognizes human SQSTM1, also known as Sequestosome-1, p62 or EBIAP. SQSTM1 is a ~62kDa ubiquitously expressed molecule bearing an N-terminal OPR domain which mediates homooligomerization and interactions with other proteins. SQSTM1 also bears a C-terminal UBA domain, responsible for binding to polyubiquitinated substrates.

      SQSTM1 is involved in the autophagic degradation of polyubiquitinated proteins (Wei et al. 2013) and in bone remodelling through activation of of osteoclasts. Mutations in the SQSTM1 gene have been implicated in Paget's disease of bone (Laurin et al. 2002)

      Mouse anti human SQSTM1, clone 2C11 has been used successfully for the identification of SQSTM1 in Hela cells by western blotting.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence0.110 ug/ml
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional SQSTM1 Antibody Formats

    Formats Clone Applications Sizes available
    SQSTM1 Antibody : Preservative Free 2C11 IF, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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