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Serum Amyloid A Antibody | SAA19

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  • Mouse anti Human Serum Amyloid A
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA6030GAE, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Human serum amyloid A, clone SAA19, specifically recognizes the human serum amyloid A (SAA) protein, an acute phase protein belonging to the SAA family which is secreted into the plasma by the liver following injury or infection.

      SAA is a widely used marker of inflamation as elevated levels of SAA may be detected in the blood of the individual following infection or tissue trauma.

      Serum amyloid A is highly conserved between species with human SAA being similar in structure to SAA in other species. Studies have been undertaken that demonstrate monoclonal antibodies raised against human SAA can be used to develop assays for measurement of SAA in domestic animals (Christensen, M, et al.).

      In addition to human SAA, clone SAA19 recognises canine, equine and feline SAA. Structurally canine, feline and equine SAA differ from human SAA in that they contain an 8 aa insert in the central region of the sequence, human SSA being 104 aa, equine SAA 110 aa, while canine and feline SAA are 111 aa.

      Clone SAA19 may be use as a capture reagent along with clone VSA34 (MCA6031GA) as the detection reagent in the development of immunoassays to detect SAA in dogs, cats and horses along with recombinant SAA (for canine, product code PDP006, for feline, product code PFP003 and for equine, product code PEP006) as standards.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Human Serum Amyloid A (SAA)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted.
    • Shelf Life
      18 months from date of despatch
    • GO Terms
      high-density lipoprotein particle
      acute-phase response
    • UniProt
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      This product may be used in a direct ELISA or as a capture reagent in a sandwich ELISA, together with MCA6031GA, as the detection reagent.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Serum Amyloid A Antibody Formats

    Formats Clone Applications Sizes available
    Serum Amyloid A Antibody : Purified SAA19 E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      Human anti Mouse IgG2a:FITCHCA037F0.1 mgF
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      Human anti Mouse IgG2a:HRPHCA037P0.1 mgE
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Dog Serum Amyloid AMCA6031GA0.1 mgE, WB
          Recombinant Dog Serum Amyloid APDP0060.1 mgE
          Recombinant Horse Serum Amyloid APEP0060.1 mgE
          Recombinant Cat Serum Amyloid APFP0030.1 mgE

          Recommended Positive Controls

            Histology Controls

              Further Reading

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