MAdCAM-1 Antibody | 314G8

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  • MAdCAM-1 Antibody | 314G8 thumbnail image 1
  • Mouse anti Human MAdCAM-1
  • Mouse anti Human MAdCAM-1:Low Endotoxin
  • Mouse anti Human MAdCAM-1:Biotin
  • Mouse anti Human MAdCAM-1:Biotin
  • Mouse anti Human MAdCAM-1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    314G8
  • Isotype
    IgG1
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2320BEdatasheet pdfdatasheet pdf0.1 mg
    MCA2320B
    MCA2320C, Edatasheet pdfdatasheet pdf0.2 mg
    MCA2320
    MCA2320ELE, FNdatasheet pdfdatasheet pdf0.5 mg
    MCA2320EL
    MCA2320BTEdatasheet pdfdatasheet pdf25 µg
    MCA2320BT
    MCA2320TC, E, Fdatasheet pdfdatasheet pdf25 µg
    MCA2320T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human MAdCAM-1 antibody, clone 314G8 recognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1) a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyer's patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria but clone 314G8 does not recognize MAdCAM-1 in these tissues. Clone 314G8 reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognized by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7 (Leung et al. 2004).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Biotin - liquid
      Purified IgG conjugated to Biotin - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Pack Size: 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.2 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.2 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      None present
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
    • Immunogen
      Recombinant soluble MAdCAM-1-fc fusion protein.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      plasma membrane
      signal transduction
      integral to membrane
      immune response
      membrane fraction
      cell adhesion
      regulation of immune response
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Frozen1/251/200
      Flow Cytometry1/101/100
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Functional Assays
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1ug/ml10ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1ug/ml10ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Frozen1/251/200
      Flow Cytometry1/101/100

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional MAdCAM-1 Antibody Formats

    Formats Clone Applications Sizes available
    MAdCAM-1 Antibody : Purified 314G8 C, E, F 0.2 mg | 25 µg
    MAdCAM-1 Antibody : Biotin 314G8 E 0.1 mg | 25 µg
    MAdCAM-1 Antibody : Low Endotoxin 314G8 E, FN 0.5 mg
    • Copyright © 2016 Bio-Rad

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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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