HLA ABC Antibody | W6/32

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HLA ABC Antibody | W6/32 gallery image 1

Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC (MCA81)

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HLA ABC Antibody | W6/32 gallery image 2

Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor® 488 (MCA81A488)

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HLA ABC Antibody | W6/32 gallery image 3

Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor® 647 (MCA81A647)

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HLA ABC Antibody | W6/32 gallery image 4

Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:FITC (MCA81F)

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HLA ABC Antibody | W6/32 gallery image 5

Staining of human peripheral blood monocytes with Mouse anti Human HLA ABC:Biotin (MCA81B)

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HLA ABC Antibody | W6/32 gallery image 6

Staining of peripheral blood lymphocytes with Mouse anti Human HLA-ABC: Low Endotoxin (MCA81EL)

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HLA ABC Antibody | W6/32 gallery image 7

Published customer image:
Mouse anti Human HLA ABC antibody, clone W6/32 used to immunoprecipitate all MHC-1 complexes from phagosomal complexes.
Image caption:
HLA-E:peptide complexes are present in phagosomal fractions. (A,B) DC were pulsed with H37Rv-eGFP or CFP102–11 peptide and the homogenate was separated using 27% percoll as described. Each fraction was freeze-thawed and tested for its ability to stimulate D160 1-23 (A) or D160 1-1B (A&B) CD8+ T cell clones in the absence of additional APC. IFN-? production was measured using ELISPOT. The mean±SEM of duplicate wells is presented. Data are representative of four experiments at various timepoints in A, and four experiments after a one hour peptide pulse in B. (C) Plasma membrane/ER fractions from Mtb–infected DC (HLA-B15+/HLA-B44-) were incubated with HLA-I matched or mismatched CD8+ T cell clones and IFN-? production was measured using ELISPOT. The mean±SEM of duplicate wells is presented and data are representative of three experiments done similarly. (D,E) Individual fractions were analyzed by flow cytometry to assess HLA-II-PE (plasma membrane) and H37Rv-eGFP fluorescence. Selected fractions are shown (D) including the peak plasma membrane fraction (#8) and phagosomal fractions (#24–27). Prior to flow cytometry, fractions were mixed with a reference latex bead population at a known concentration. Equal numbers of latex bead events were collected for all fractions and used to quantify the number of plasma membrane and phagosome particles (E) as described in Materials and Methods. (F) HLA-I was immunoprecipitated from plasma membrane or phagosome fractions using W6/32. After dilution of the plasma membrane sample to give similar levels of HC10 staining, the presence of HLA-I alleles was assessed using antibodies to pan-HLA-I (HC10), HLA-A2 (HCA2), and HLA-E (MEM-E/02). The numbers below blots indicate the relative intensity of the bands as described in Materials and Methods.
From: Grotzke JE, Harriff MJ, Siler AC, Nolt D, Delepine J, et al. (2009) The Mycobacterium tuberculosis Phagosome Is a HLA-I Processing Competent Organelle.
PLoS Pathog 5(4): e1000374.

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  • Mouse anti Human HLA ABC:ALEXA Fluor® 647
  • Mouse anti Human HLA ABC:RPE
  • Mouse anti Human HLA ABC:FITC
  • Mouse anti Human HLA ABC:HRP
  • Mouse anti Human HLA ABC:Biotin
  • Mouse anti Human HLA ABC:Low Endotoxin
  • Mouse anti Human HLA ABC:ALEXA Fluor® 488
  • Mouse anti Human HLA ABC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    W6/32
  • Isotype
    IgG2a
8 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA81FFdatasheet pdfdatasheet pdf0.1 mg
    MCA81F
    MCA81PC *datasheet pdfdatasheet pdf0.1 mg
    MCA81P
    MCA81BFdatasheet pdfdatasheet pdf0.1 mg
    MCA81B
    MCA81C *, E, F, IF, IPdatasheet pdfdatasheet pdf0.2 mg
    MCA81
    MCA81ELC *, E, F, FN, IF, IPdatasheet pdfdatasheet pdf0.5 mg
    MCA81EL
    MCA81PEFdatasheet pdfdatasheet pdf100 Tests
    MCA81PE
    MCA81A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA81A647
    MCA81A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA81A488
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human HLA ABC antibody, clone W6/32 recognizes an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells.

      Mouse anti Human HLA ABC antibody, clone W6/32 is routinely tested in flow cytometry on human peripheral blood lymphocytes.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Macaqueyes
      Bovineyes
      Cynomolgus monkeyyes
      Baboonyes
      Rhesus Monkeyyes
      Chimpanzeeyes
      Mouseno
      Chickenno
      Amphibiano
      Gorillayes
      Shrewyes
      Goatno
      Dogno
      Guinea Pigno
      Rabbitno
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised.
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG conjugated to Biotin - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
      Purified IgG - liquid
    • Reconstitution
      Reconstitute with 1ml distilled water.
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.01% Thiomersal
      50% HRP Stabiliser (BUF052A)
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      None present
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
    • Immunogen
      Purified human tonsil lymphocyte membranes.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.05 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1/1-Ag4.1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry1/501/100
      Functional Assays
      Immunofluorescence
      Immunohistology - Paraffin
      Immunoprecipitation
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry1/501/100
      Immunofluorescence
      Immunohistology - Paraffin
      Immunoprecipitation
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Tonsil
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Tonsil
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Tonsil
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional HLA ABC Antibody Formats

    Formats Clone Applications Sizes available
    HLA ABC Antibody : Alexa Fluor® 647 W6/32 F 100 Tests/1ml
    HLA ABC Antibody : Low Endotoxin W6/32 C *, E, F, FN, IF, IP 0.5 mg
    HLA ABC Antibody : HRP W6/32 C * 0.1 mg
    HLA ABC Antibody : Purified W6/32 C *, E, F, IF, IP 0.2 mg
    HLA ABC Antibody : Alexa Fluor® 488 W6/32 F 100 Tests/1ml
    HLA ABC Antibody : FITC W6/32 F 0.1 mg
    HLA ABC Antibody : Biotin W6/32 F 0.1 mg
    HLA ABC Antibody : RPE W6/32 F 100 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

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        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
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          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
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          Human SeroblockBUF070A50 TestF
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          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          Recommended Positive Controls

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              References

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