Eosinophil Major Basic Protein Antibody | BMK-13

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  • Mouse anti Human Eosinophil Major Basic Protein
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  • Product Type
    Monoclonal Antibody
  • Clone
    BMK-13
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5751C *, P *datasheet pdfdatasheet pdf0.1 mg
    MCA5751
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Eosinophil Major Basic Protein antibody, clone BMK-13 recongises the Eosinophil Major Basic Protein (EMBP), a 117 amino acid protein, corresponding to residues 106-222 of Bone marrow proteoglycan (precursor). Mouse anti Human Eosinophil Major Basic Protein antibody, clone BMK-13 stains both resting and activated eosinophils of bronchial and skin sections of allergic and normal sites and may be considered a Pan eosinophil marker. Mouse anti Human Eosinophil Major Basic Protein antibody, clone BMK-13 cross reacts weakly with basophils which also contain low levels of EMBP. No cross reactivity with other human cells or proteins has been noted.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      Guinea PigWeak
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.1% Bovine Serum Albumin
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      transport vesicle
      sugar binding
      immune response
      defense response to bacterium
      heparin binding
      extracellular region
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen(1)1/201/50
      Immunohistology - Paraffin(2)1/201/50
      (1)
      It is recommended that sections are fixed in a 1:1 mixture of acetone and methanol and air-dried for 1 hour. Good results may be achieved via staining with the APAAP method.
      (2)
      This product requires enzymatic pre-treatment of paraffin sections prior to staining. Pepsin is recommended for this purpose. NB. Heat-mediated antigen retrieval methods should not be used.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Eosinophil Major Basic Protein Antibody Formats

    Formats Clone Applications Sizes available
    Eosinophil Major Basic Protein Antibody : Purified BMK-13 C *, P * 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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