Desmoplakin 1/2 Antibody | DP-2.15

Desmoplakin 1/2 Antibody | DP-2.15 gallery image 1

Published customer image:
Mouse anti Human desmoplakin 1/2 antibody, clone DP-2.15 used for the detection of desmoplakin expressing human cardiomyocytes by immunofluorescence.
Image caption:
Cardiac matrix coatings increase hESC derived cardiomyocyte maturation compared to the standard gelatin substrate. hESC-derived cardiomyocytes were cultured on gelatin (a and c) or cardiac matrix (b and d) and stained for Titin M8 (red), desmoplakin (green), or nuclei (blue). Cell cluster area (e), number of nuclei per cluster (f), and average desmoplakin positive area (g) were measured and compared between the two coatings. Cardiomyocytes had an increased cluster area and number of nuclei at both day 35 and 112. At day 112, the average desmoplakin immunoreactive area was significantly greater on the cardiac matrix, with localization observed at the lateral ends of the cardiomyocytes (arrows), indicating the formation of desmosomes and a more mature phenotype. **p<0.01.

From: DeQuach JA, Mezzano V, Miglani A, Lange S, Keller GM, et al. (2010) Simple and High Yielding Method for Preparing Tissue Specific Extracellular Matrix Coatings for Cell Culture.
PLoS ONE 5(9): e13039.

Desmoplakin 1/2 Antibody | DP-2.15 gallery image 2

Published customer image:
Mouse anti Desmoplakin 1/2 antibody used for the detection of desmoplakin expressing cells in porcine meninges by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical analysis of the porcine meninges. The meninges composed of the dura mater (A–G), the arachnoid mater (H–I) and the pia mater (J–M) in formalin fixed tissue. The porcine spinal cord was cryosectioned and the porcine dura mater (N–O) was stained. The tissue sections were labeled for: (A) von Willebrand factor, (B and K) fibronectin, (C and J) vimentin, (D) laminin, (E) collagen I, (F and M) collagen II, (G, H, and L) integrin 1ß, (I) Desmoplakin, (N) collagen III, (O) E-cadherin. Images were captured at ×200 magnification.

From: Papageorgiou I, Marsh R, Tipper JL, Hall RM, Fisher J, Ingham E. Interaction of micron and nano-sized particles with cells of the dura mater.
J Biomed Mater Res B Appl Biomater. 2014 Oct;102(7):1496-505.

Desmoplakin 1/2 Antibody | DP-2.15 gallery image 3

Published customer image:
Mouse anti Human desmoplakin 1/2 antibody, clone DP-2.15 (2722-5204) used to demonstrate desmoplakin expression in BeWo cells in vitro by immunofluorescence.
Image caption:
Validation of trophoblastic properties in newly derived BeWo-NG cells.
c) BeWo-NG cells can undergo syncytilialization after FSK A stimulation. Confluent cells were treated with 10 μmol/L FSK A for 24 h. Cells were fixed in cold acetone/methanol and permeabilization using 0.1% saponin in 1% BSA. Cells were then stained with desmoplakin specific primary antibody and Alexa 488 conjugated secondary antibody before visualizationusing fluorescent microscopy. The nuclei of cells were counterstained with DAPI nuclear dye. Red lines are drawn around the boundary of Syncytialized cells. Micrographs were taken under 200X magnification

From: Yung HW, Alnæs-Katjavivi P, Jones CJ, El-Bacha T, Golic M, Staff AC, Burton GJ.
Placental endoplasmic reticulum stress in gestational diabetes: the potential for therapeutic intervention with chemical chaperones and antioxidants.
Diabetologia. 2016 Oct;59(10):2240-50.

  • Desmoplakin 1/2 Antibody | DP-2.15 thumbnail image 1
  • Desmoplakin 1/2 Antibody | DP-2.15 thumbnail image 2
  • Desmoplakin 1/2 Antibody | DP-2.15 thumbnail image 3
  • Mouse anti Desmoplakin 1/2
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    2722-5204C, IF, WBdatasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Desmoplakin 1/2 antibody, clone DP-2.15 recognizes both desmoplakin 1 and 2 from stratified epithelia, simple epithelia including glands, urothelium, thymic reticular epithelium, hepatocytes, intercalated disks of myocardium and arachnoid cells of meninges. Mouse anti Desmoplakin 1/2 antibody, clone DP-2.15 can be used for the detection of primary and metastatic carcinomas.
      Mouse anti Desmoplakin 1/2 antibody, clone DP-2.15 recognizes the N terminus of the rod domain of desmoplakin ( Bornslaeger et al. 1996).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Native bovine desmoplakin 1 and 2 purified from bovine snout desmosomes.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized Balb/c mice were fused with cells from the NS0 mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      keratinocyte differentiation
      cellular component disassembly involved in apoptosis
      structural constituent of cytoskeleton
      protein binding, bridging
      peptide cross-linking
      cornified envelope
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Desmoplakin 1/2 Antibody Formats

    Formats Clone Applications Sizes available
    Desmoplakin 1/2 Antibody : Purified DP-2.15 C, IF, WB 50 µg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF, WB
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images


              Write your review

              You may also be interested in...