Desmoplakin 1/2 Antibody | DP-2.15

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Desmoplakin 1/2 Antibody | DP-2.15 gallery image 1

Published customer image:
Mouse anti Human desmoplakin 1/2 antibody, clone DP-2.15 used for the detection of desmoplakin expressing human cardiomyocytes by immunofluorescence.
Image caption:
Cardiac matrix coatings increase hESC derived cardiomyocyte maturation compared to the standard gelatin substrate. hESC-derived cardiomyocytes were cultured on gelatin (a and c) or cardiac matrix (b and d) and stained for Titin M8 (red), desmoplakin (green), or nuclei (blue). Cell cluster area (e), number of nuclei per cluster (f), and average desmoplakin positive area (g) were measured and compared between the two coatings. Cardiomyocytes had an increased cluster area and number of nuclei at both day 35 and 112. At day 112, the average desmoplakin immunoreactive area was significantly greater on the cardiac matrix, with localization observed at the lateral ends of the cardiomyocytes (arrows), indicating the formation of desmosomes and a more mature phenotype. **p<0.01.

From: DeQuach JA, Mezzano V, Miglani A, Lange S, Keller GM, et al. (2010) Simple and High Yielding Method for Preparing Tissue Specific Extracellular Matrix Coatings for Cell Culture.
PLoS ONE 5(9): e13039.

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Desmoplakin 1/2 Antibody | DP-2.15 gallery image 2

Published customer image:
Mouse anti Desmoplakin 1/2 antibody used for the detection of desmoplakin expressing cells in porcine meninges by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical analysis of the porcine meninges. The meninges composed of the dura mater (A–G), the arachnoid mater (H–I) and the pia mater (J–M) in formalin fixed tissue. The porcine spinal cord was cryosectioned and the porcine dura mater (N–O) was stained. The tissue sections were labeled for: (A) von Willebrand factor, (B and K) fibronectin, (C and J) vimentin, (D) laminin, (E) collagen I, (F and M) collagen II, (G, H, and L) integrin 1ß, (I) Desmoplakin, (N) collagen III, (O) E-cadherin. Images were captured at ×200 magnification.

From: Papageorgiou I, Marsh R, Tipper JL, Hall RM, Fisher J, Ingham E. Interaction of micron and nano-sized particles with cells of the dura mater.
J Biomed Mater Res B Appl Biomater. 2014 Oct;102(7):1496-505.

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  • Mouse anti Desmoplakin 1/2
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    DP-2.15
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    2722-5204C, IF, WBdatasheet pdfdatasheet pdf50 µg
    2722-5204
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Desmoplakin 1/2 antibody, clone DP-2.15 recognizes both desmoplakin 1 and 2 from stratified epithelia, simple epithelia including glands, urothelium, thymic reticular epithelium, hepatocytes, intercalated disks of myocardium and arachnoid cells of meninges. Mouse anti Desmoplakin 1/2 antibody, clone DP-2.15 can be used for the detection of primary and metastatic carcinomas.
      Mouse anti Desmoplakin 1/2 antibody, clone DP-2.15 recognizes the N terminus of the rod domain of desmoplakin ( Bornslaeger et al. 1996).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Bovineyes
      Chickenyes
      Mouseyes
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Native bovine desmoplakin 1 and 2 purified from bovine snout desmosomes.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized Balb/c mice were fused with cells from the NS0 mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      keratinocyte differentiation
      cellular component disassembly involved in apoptosis
      desmosome
      structural constituent of cytoskeleton
      protein binding, bridging
      peptide cross-linking
      cornified envelope
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
      Immunohistology - Frozen
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Desmoplakin 1/2 Antibody Formats

    Formats Clone Applications Sizes available
    Desmoplakin 1/2 Antibody : Purified DP-2.15 C, IF, WB 50 µg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence

              References

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