CD8 Antibody | RPA-T8

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  • Mouse anti Human CD8:RPE
  • Mouse anti Human CD8
  • Mouse anti Human CD8:FITC
  • Mouse anti Human CD8
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    RPA-T8
  • Isotype
    IgG1
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4609FFdatasheet pdfdatasheet pdf0.1 mg
    MCA4609F
    MCA4609GAC, Fdatasheet pdfdatasheet pdf0.1 mg
    MCA4609GA
    MCA4609PEFdatasheet pdfdatasheet pdf100 Tests/0.5ml
    MCA4609PE
    MCA4609TC, Fdatasheet pdfdatasheet pdf25 µg
    MCA4609T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD8 monoclonal antibody, clone RPA-T8 recognizes the alpha-chain of the human CD8 antigen, a ~32kDa single pass type 1 membrane glycoprotein containing a single Ig-like V-type domain. CD8α forms either homodimers or disulphide bond linked heterodimers with the CD8β chain. CD8 is expressed by thymocytes, the cytotoxic-suppressor subset of T cells and weakly by NK cells. CD8 functions as a receptor for MHC Class I molecules.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Chimpanzeeyes
      Baboonyes
      Cynomolgus monkeyyes
      Rhesus Monkeyyes
      Macaqueyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.2% Bovine Serum Albumin
      0.09%Sodium Azide
      0.09% Sodium Azide (NaN3)
      0.09%Sodium Azide
    • Purity
    • Approx. Protein Concentrations
      Pack Size: 25 µgIgG concentration 0.5mg/ml
      Pack Size: 0.1 mgIgG concentration 0.5 mg/ml
      IgG concentration 0.5 mg/ml
      Pack Size: 25 µgIgG concentration 0.5mg/ml
      Pack Size: 0.1 mgIgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 25 µgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.1 mgStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 25 µgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.1 mgStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
      18 months from date of despatch.
      12 months from date of despatch
      12 months from date of despatch
    • GO Terms
      extracellular region
      T cell receptor complex
      transmembrane receptor protein tyrosine kinase signaling pathway
      antigen processing and presentation
      coreceptor activity
      integral to plasma membrane
      MHC class I protein binding
      regulation of immune response
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      NeatPack Size: 0.1 mg
      1/10 Pack Size: 25 µg
      1/5Pack Size: 0.1 mg
      1/20Pack Size: 25 µg
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/51/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      NeatPack Size: 0.1 mg
      1/10 Pack Size: 25 µg
      1/5Pack Size: 0.1 mg
      1/20Pack Size: 25 µg
      Immunohistology - Frozen

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1 x 106 cells or cells or 100ul human whole blood.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD8 Antibody Formats

    Formats Clone Applications Sizes available
    CD8 Antibody : Purified RPA-T8 C, F 0.1 mg | 25 µg
    CD8 Antibody : RPE RPA-T8 F 100 Tests/0.5ml
    CD8 Antibody : FITC RPA-T8 F 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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