CD8 Antibody | 4B11

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CD8 Antibody | 4B11 gallery image 1

Published customer image:
Mouse antiHuman CD8 antibody, clone 4B11 (MCA1817) used for the identification of CD8 expressing cells in Buruli ulcer secondary lesions by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Presence of B-cell clusters in the secondary lesions.
A: Band of CD20 positive B-cells in sections of ulcer 2 of patient 1. B–E: serial sections of nodule 3 of patient 2 with a small dense cluster of CD20 positive B-cells (B) surrounded by CD14 positive macrophages/monocytes (C) and few interspersed CD3 positive T-cells (D) from which the majority was CD8 negative (E). Higher magnification (F–I) revealed a very dense package of the B-cells.

From: Ruf M-T, Chauty A, Adeye A, Ardant M-F, Koussemou H, Johnson RC, et al. (2011)
Secondary Buruli Ulcer Skin Lesions Emerging Several Months after Completion of Chemotherapy: Paradoxical Reaction or Evidence for Immune Protection?
PLoS Negl Trop Dis 5(8): e1252.

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CD8 Antibody | 4B11 gallery image 2

CD8 - MCA1817T - Tonsil. Formalin fixed, paraffin processed tissue. Mag. 40X

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  • CD8 Antibody | 4B11 thumbnail image 1
  • CD8 Antibody | 4B11 thumbnail image 2
  • Mouse anti Human CD8
  • Mouse anti Human CD8
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    4B11
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1817TC, P *datasheet pdfdatasheet pdf0.1 ml
    MCA1817T
    MCA1817C, P *, WBdatasheet pdfdatasheet pdf1 ml
    MCA1817
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD8 antibody, clone 4B11 recognizes the human CD8 cell surface antigen, a ~32 kDa glycoprotein expressed by the cytotoxic/suppressor subset of T-cells and weakly by NK cells. Mouse anti Human CD8 antibody, clone 4B11 was raised based on an earlier successful strategy used to generate rabbit polyclonal antibodies against human CD8 employing the same immunizing peptide (Mason et al. 1992).

      Mouse anti Human CD8 antibody, clone 4B11 has been reported as being suitable for use in Western blotting (Williamson et al. 1998) .
    • Intended Use
    • Target Species
      Human
    • Product Form
      Tissue Culture Supernatant - liquid.
      Tissue Culture Supernatant - liquid.
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
    • Immunogen
      Synthetic peptide derived from the carboxy terminal region of the humanCD8 alpha chain coupled to a N-terminal cysteine, with the sequence C-KSDGKPSLSARYV. The peptide was coupled to bovine serum albuman and keyhole limpet hemocyanin.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Fusion Partners
      Spleen cells from immunised mice were fused with cells of a mouse p3-NS1-Ag4-1 myeloma cell line.
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of despatch.
    • GO Terms
      coreceptor activity
      transmembrane receptor protein tyrosine kinase signaling pathway
      extracellular region
      T cell receptor complex
      MHC class I protein binding
      antigen processing and presentation
      regulation of immune response
      integral to plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/20
      1/50Pack Size: 1 ml
      1/40Pack Size: 0.1 ml
      Western Blotting1/25
      Immunohistology - Paraffin(1)1/20
      1/50Pack Size: 1 ml
      1/40Pack Size: 0.1 ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

      Tris/EDTA buffer pH 8.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/20
      1/50Pack Size: 1 ml
      1/40Pack Size: 0.1 ml
      Western Blotting1/25
      Immunohistology - Paraffin(1)1/20
      1/50Pack Size: 1 ml
      1/40Pack Size: 0.1 ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

      Tris/EDTA buffer pH 8.0 is recommended for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Human Tonsil
    • Histology Positive Control Tissue
      Human Tonsil
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD8 Antibody Formats

    Formats Clone Applications Sizes available
    CD8 Antibody : S/N 4B11 C, P *, WB 1 ml | 0.1 ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Human Tonsil
              Human Tonsil

              Source Reference

              References

              Further Reading

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