CD62E/CD62P Antibody | 1.2B6

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CD62E/CD62P Antibody | 1.2B6 gallery image 1

Staining of thrombin activated human peripheral blood platelets with Mouse anti Human CD62E/CD62P (MCA883)

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CD62E/CD62P Antibody | 1.2B6 gallery image 2

Staining of thrombin activated human peripheral blood platelets with Mouse anti Human CD62E/CD62P: FITC (MCA883F)

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  • CD62E/CD62P Antibody | 1.2B6 thumbnail image 1
  • CD62E/CD62P Antibody | 1.2B6 thumbnail image 2
  • Mouse anti Human CD62E/CD62P
  • Mouse anti Human CD62E/CD62P
  • Mouse anti Human CD62E/CD62P:FITC
  • Mouse anti Human CD62E/CD62P:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    1.2B6
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA883FFdatasheet pdfdatasheet pdf0.1 mg
    MCA883F
    MCA883C *, E, F, IP, WB*datasheet pdfdatasheet pdf0.2 mg
    MCA883
    MCA883TC *, E, F, IP, WB*datasheet pdfdatasheet pdf25 µg
    MCA883T
    MCA883FTFdatasheet pdfdatasheet pdf25 µg
    MCA883FT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human CD62E/CD62P antibody, clone 1.2B6 recognizes the human CD62E and CD62P cell surface antigens.

      Although previously thought to recognize only human CD62E, recent data (Goda et al. 2003) shows that Mouse anti Human CD62E/CD62P antibody, clone 1.2B6 also recognizes human CD62P, binding to a common epitope shared by these members of the selectin family.

      Clone 1.2B6 reacts with porcine E-selectin (CD62E) but not with porcine P-selectin (Stocker et al. 2000).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA10ug/ml
      Flow Cytometry10ug/ml
      Immunohistology - Paraffin
      Immunoprecipitation
      Immunohistology - Frozen(1)1ug/ml10ug/ml
      Western Blotting(2)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
      (2)
      Clone 1.2B6 recognizes human CD62E/CD62P only under non-reducing conditions.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA10ug/ml
      Flow Cytometry10ug/ml
      Immunohistology - Paraffin
      Immunoprecipitation
      Immunohistology - Frozen(1)1ug/ml10ug/ml
      Western Blotting(2)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
      (2)
      Clone 1.2B6 recognizes human CD62E/CD62P only under non-reducing conditions.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Human Tonsil
    • Histology Positive Control Tissue
      Human Tonsil
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD62E/CD62P Antibody Formats

    Formats Clone Applications Sizes available
    CD62E/CD62P Antibody : Purified 1.2B6 C *, E, F, IP, WB* 0.2 mg | 25 µg
    CD62E/CD62P Antibody : FITC 1.2B6 F 0.1 mg | 25 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
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      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
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      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
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      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
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      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
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      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
        MCA928F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
        MCA928F

        Useful Reagents

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          Human SeroblockBUF070B200 TestF
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          Human SeroblockBUF070A50 TestF
          BUF070A
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          Human SeroblockBUF070B200 TestF
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          Human SeroblockBUF070A50 TestF
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          Recommended Positive Controls

            Histology Controls

              Human Tonsil
              Human Tonsil
              • Application NameReference Images
                Flow Cytometry

              References

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