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CD56 antibody | MEM-188

Product Code Applications Pack Size List Price Your Price Qty
MCA2046GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F IP WB * 0.1 mg loader
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loader
MCA2046
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F IP WB * 0.2 mg loader
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loader

Mouse anti Human CD56 antibody, clone MEM-188 recognizes CD56, a 140kDa cell surface glycoprotein expressed primarily by natural killer cells and a subset of T cells in peripheral blood.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/50 Pack Size: 0.1 mg
1/100 Pack Size: 0.2 mg
Immunohistology - Frozen 1 1/40
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting 2 Non-reducing conditions
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
  2. 2 MEM-188 recognizes CD56 under non-reducing conditions.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

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Description Mouse IgG2a Negative Control

References for CD56 antibody

  1. Costa, P.et al (1997) CD56 Workshop Panel Report.
    In: Leucocyte typing VI. White cell differentiation antigens. Garland Publishing Inc. New York and London. 271-2.
  2. Prager, E. et al. (2001) Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells.
    J Immunol. 166 (4): 2364-71.
  3. Staffler G et al. (2003) Selective inhibition of T cell activation via CD147 through novel modulation of lipid rafts.
    J Immunol. 171 (4): 1707-14.
  4. Crisan, M. et al. (2008) A perivascular origin for mesenchymal stem cells in multiple human organs.
    Cell Stem Cell. 3: 301-13.
  5. Newman, K.C. et al. (2006) Cross-talk with myeloid accessory cells regulates human natural killer cell interferon-gamma responses to malaria.
    PLoS Pathog. 2: e118.
  6. Apps, R. et al. (2011) Ex vivo functional responses to HLA-G differ between blood and decidual NK cells.
    Mol Hum Reprod. 17: 577-86.
  7. Aspinall, A.I. et al. (2010) CX(3)CR1 and vascular adhesion protein-1-dependent recruitment of CD16(+) monocytes across human liver sinusoidal endothelium.
    Hepatology. 51: 2030-9.
  8. Rodriguez JM et al. (2015) PyNTTTTGT and CpG Immunostimulatory Oligonucleotides: Effect on Granulocyte/Monocyte Colony-Stimulating Factor (GM-CSF) Secretion by Human CD56+ (NK and NKT) Cells.
    PLoS One. 10 (2): e0117484.
  9. View The Latest Product References

Synonyms
N-CAM
RRID
AB_323329
UniProt
P13591
Entrez Gene
NCAM1

MCA2046GA

149541 152256

MCA2046

152255 1611

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