CD53 Antibody | MEM-53

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CD53 Antibody | MEM-53 gallery image 1

Published customer image:
Mouse anti Human CD53 antibody, clone MEM-53 used for the evaluation of CD53 expression on white blood cell subsets by flow cytometry.
Image caption:
Expression of CD37 and CD53 on immune cell subsets in blood. a Flow cytometry analysis of expression of CD37 or CD53 (black line) on CD4 and CD8 T cells, B cells, monocytes and NK cells versus isotype control (gray line). Gating strategy is presented in Supplementary Figure 1. Expression levels of CD37 (b) and CD53 (c) were normalized for isotype staining by background subtraction. Experiments were performed with PBLs from three healthy donors. Data present mean ± SD. d Flow cytometry analysis of expression of CD37 or CD53 (black line) on mDCs (BDCA1+CD19-) or pDCs (BDCA2+) versus isotype control (gray line). Expression levels of CD37 (e) and CD53 (f) were normalized for isotype staining by background subtraction. Experiments were performed with PBLs from two healthy donors. Data present mean ± SD

From: de Winde CM, Zuidscherwoude M, Vasaturo A, van der Schaaf A, Figdor CG, van Spriel AB. Multispectral imaging reveals the tissue distribution of tetraspanins in human lymphoid organs. Histochem Cell Biol. 2015 May 8. [Epub ahead of print]

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CD53 Antibody | MEM-53 gallery image 2

Published customer image:
Mouse anti Human CD53 antibody, clone MEM-53 used for the evaluation of CD53 expression on erythroid cultures by flow cytometry.
Image caption:
Erythroid culture characterisation and expression of tetraspanins and integrins during terminal maturation. A. Temporal expression of erythroid-specific markers, Kell, GPA and AE1 and morphology of the culture at the same time points. AE1 was tested from day 5 onwards. B. Tetraspanin and integrin profile of the same cultures as shown in A. Results are depicted from one culture where directly conjugated antibodies were used (days 3 and 4) and a second culture with indirectly labelled antibodies (day 5 onwards). The y-axis scale is linear to 350 counts; the x-axis is logarithmic to 104. Images were captured on a Leica DM750 microscope, x20 magnification, using Image-Pro Express 6.0 software.

From: Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, Chasis JA, et al. (2013) Tetraspanins CD81 and CD82 Facilitate a4ß1-Mediated Adhesion of Human Erythroblasts to Vascular Cell Adhesion Molecule-1.
PLoS ONE 8(5): e62654.

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CD53 Antibody | MEM-53 gallery image 3

Published customer image:
Mouse anti Human CD53 antibody, clone MEM-53 used for the evaluation of CD53 expression on erythroid cultures by immunoprecipitation.
Image caption:
Several anti-tetraspanin antibodies co-precipitate β1 integrins from HEL cells solubilised in Brij-97. Precipitates were prepared from HEL cells solubilised in different detergents in the presence of Mn2+. CD53, MEM-53; CD63, MEM-259; CD81, 454720; CD82, TS82b; CD151, IIG5a; α4, HP2/1; αL, TS1/22; αIIb, PAB-1. Precipitates were run on 7.5% non-reduced gels.

From: Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, Chasis JA, et al. (2013) Tetraspanins CD81 and CD82 Facilitate a4ß1-Mediated Adhesion of Human Erythroblasts to Vascular Cell Adhesion Molecule-1.
PLoS ONE 8(5): e62654.

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CD53 Antibody | MEM-53 gallery image 4

Published customer image:
Mouse anti Human CD53 antibody, clone MEM-53 used for the evaluation of CD53 distribution in monocytes using immunofluorescence.
Image caption:
Subcellular localization of CD37 and CD53. Localization of a CD37 or b CD53 (green) in monocytes was studied by dual staining with calreticulin (ER), syntaxin 13 (endosomes) or Lamp1 (lysosomes) (red). Merge: co-localization in yellow (white arrows). Scale bar 5 μm

From: de Winde CM, Zuidscherwoude M, Vasaturo A, van der Schaaf A, Figdor CG, van Spriel AB. Multispectral imaging reveals the tissue distribution of tetraspanins in human lymphoid organs. Histochem Cell Biol. 2015 May 8. [Epub ahead of print]

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CD53 Antibody | MEM-53 gallery image 5

Published customer image:
Mouse anti Human CD53 antibody, clone MEM-53 used for the evaluation of CD53 expression on erythroid cultures by immunoprecipitation and western blotting.
Image caption:
Co-precipitation of ß1 and ß3 integrins by tetraspanins with different cations from HEL cells. Precipitates were prepared from HEL cells solubilised in Brij-97 in the presence of EDTA or cations. ß1 is co-precipitated by CD63, CD81, CD82 and CD151 while ß3 is co-precipitated by all tetraspanins in the presence of Mn2+. CD151 co-precipitates ß1 under all conditions. Integrins were separated on 7.5% gels, tetraspanin controls on 12% gels, both non-reducing conditions. CD53, MEM-53; CD63, MEM-259; CD81, 454720; CD82, TS82b; CD151, IIG5a; a4, HP2/1; aL, TS1/22; aIIb, PAB-1.

From: Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, Chasis JA, et al. (2013) Tetraspanins CD81 and CD82 Facilitate a4ß1-Mediated Adhesion of Human Erythroblasts to Vascular Cell Adhesion Molecule-1.
PLoS ONE 8(5): e62654.

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CD53 Antibody | MEM-53 gallery image 6

Figure A. RPE-A647 tandem conjugated Mouse anti Human CD3 (MCA463P647) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE-A647 tandem conjugated Mouse anti Human CD3 (MCA463P647) and FITC conjugated Mouse anti Human CD53 (MCA723F). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

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  • Mouse anti Human CD53
  • Mouse anti Human CD53
  • Mouse anti Human CD53:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    MEM-53
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA723GC, F, IP, WB*datasheet pdfdatasheet pdf0.2 mg
    MCA723G
    MCA723FFdatasheet pdfdatasheet pdf100 Tests
    MCA723F
    MCA723GTC, F, IP, WB*datasheet pdfdatasheet pdf25 µg
    MCA723GT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD53 antibody, clone MEM-53 recognizes the human CD53 cell surface antigen, also known as Tetraspanin-25. CD53 is a 219 amino acid multi pass transmembrane glycoprotein of 32-42 kDa. CD53 is expressed by all leucocytes, but is absent from erythrocytes and platelets.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
      Purified IgG prepared by affinity chromatography on Protein G
      Purified IgG prepared by ion exchange chromatography
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1 mg/ml
      IgG concentration 1 mg/ml
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      integral to membrane
      plasma membrane
      signal transduction
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/25
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting(1)
      (1)
      Clone MEM-53 recognizes human CD53 under non-reducing conditions.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/25
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting(1)
      (1)
      Clone MEM-53 recognizes human CD53 under non-reducing conditions.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD53 Antibody Formats

    Formats Clone Applications Sizes available
    CD53 Antibody : FITC MEM-53 F 100 Tests
    CD53 Antibody : Purified MEM-53 C, F, IP, WB* 0.2 mg | 25 µg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

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        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
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              References

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