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CD49f antibody | 450-30A

Product Code Applications Pack Size List Price Your Price Qty
MCA1457
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F WB 0.2 mg loader
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loader
MCA1457T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F WB 25 µg loader
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loader
MCA1457GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F WB 0.1 mg loader
List Price Your Price
loader

Mouse anti Human CD49f antibody, clone 450-30A recognizes the human VLA-6 cell surface antigen, also known as the alpha 6 integrin and as CD49f.

CD49f is expressed by platelets, weakly by monocytes and by a subset of lymphocytes.

CD49f is also widely expressed on epithelial tissues.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
MCA1457, MCA1457T: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA1457GA: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
alpha 6 beta 4 integrin purified from A431 cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/50
Immunohistology - Frozen 1 1/50 1/100
Immunohistology - Paraffin
Western Blotting
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl
Histology Positive Control Tissue
Human tonsil

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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Mouse IgG1 Negative Control

References for CD49f antibody

  1. Kennel, S.J. et al. (1990) Second generation monoclonal antibodies to the human integrin alpha 6 beta 4.
    Hybridoma. 9 (3): 243-55.
  2. Cavers, M. et al. (2002) Differential expression of beta1 and beta2 integrins and L-selectin on CD4+ and CD8+ T lymphocytes in human blood: comparative analysis between isolated cells, whole blood samples and cryopreserved preparations.
    Clin Exp Immunol. 127: 60-5.
  3. Liu, L. et al. (2003) Priming of eosinophil migration across lung epithelial cell monolayers and upregulation of CD11b/CD18 are elicited by extracellular Ca2+.
    Am J Respir Cell Mol Biol. 28: 713-21.
  4. Maurice, S. et al. (2007) Isolation of progenitor cells from cord blood using adhesion matrices.
    Cytotechnology. 54: 121-33.
  5. Kaczmarek, M. et al. (2011) Evaluation of the phenotype pattern of macrophages isolated from malignant and non-malignant pleural effusions.
    Tumour Biol. 32: 1123-32.
  6. López, J. et al. (2012) Cancer-initiating cells derived from established cervical cell lines exhibit stem-cell markers and increased radioresistance.
    BMC Cancer. 12: 48.
  7. Keller, P.J. et al. (2012) Defining the cellular precursors to human breast cancer.
    Proc Natl Acad Sci U S A. 109: 2772-7.
  8. Aldridge, V. et al. (2012) Human mesenchymal stem cells are recruited to injured liver in a β1-integrin and CD44 dependent manner.
    Hepatology. 56 (3): 1063-73.
  9. View The Latest Product References
  10. Steinberg, F. et al. (2012) SNX17 protects integrins from degradation by sorting between lysosomal and recycling pathways.
    J Cell Biol. 197 (2): 219-30.
  11. Goyer, B. et al. (2018) Extracellular Matrix and Integrin Expression Profiles in Fuchs Endothelial Corneal Dystrophy Cells and Tissue Model.
    Tissue Eng Part A. 24 (7-8): 607-15.
  12. Amirian, M. et al. (2022) VASA protein and gene expression analysis of human non-obstructive azoospermia and normal by immunohistochemistry, immunocytochemistry, and bioinformatics analysis.
    Sci Rep. 12 (1): 17259.
  13. Hashemi Karoii, D. et al. (2023) Altered G-Protein Transduction Protein Gene Expression in the Testis of Infertile Patients with Nonobstructive Azoospermia.
    DNA Cell Biol. 42 (10): 617-37.

Flow Cytometry

Synonyms
Integrin Alpha 6 Chain
VLA-6
RRID
AB_321464
UniProt
P23229
Entrez Gene
ITGA6

MCA1457

165881 1801

MCA1457T

0514 155745 158706 166440

MCA1457GA

1801

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