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CD44v6 Antibody | VFF-7

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CD44v6 Antibody | VFF-7 gallery image 1

Immunoperoxidase staining of formalin fixed, paraffin embedded human breast cancer tissue with Mouse anti Human CD44v6 antibody (MCA1730). Low power

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CD44v6 Antibody | VFF-7 gallery image 2

Immunoperoxidase staining of formalin fixed, paraffin embedded human breast cancer tissue with Mouse anti Human CD44v6 antibody (MCA1730). High power

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  • CD44v6 Antibody | VFF-7 thumbnail image 1
  • CD44v6 Antibody | VFF-7 thumbnail image 2
  • Mouse anti Human CD44v6
  • Mouse anti Human CD44v6:FITC

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  • Product Type
    Monoclonal Antibody
  • Clone
    VFF-7
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1730C, F, P*, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA1730
    MCA1730FFdatasheet pdfdatasheet pdf100 Tests
    MCA1730F
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD44v6 antibody, clone VFF-7 specifically recognizes an epitope encoded by exon v6 on the variant portion of human CD44.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.02%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Glutathione S Transferase (GST) fusion protein corresponding to the variable domains (v3 to v10) of human CD44.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1 mg/ml
      IgG concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Tris buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c micewere fused with cells of the P3X63Ag8.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/200
      Immunohistology - Frozen1/4001/500
      Western Blotting
      Immunohistology - Paraffin(1)1/200
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD44v6 Antibody Formats

    Formats Clone Applications Sizes available
    CD44v6 Antibody : Purified VFF-7 C, F, P*, WB 0.1 mg
    CD44v6 Antibody : FITC VFF-7 F 100 Tests
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
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      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF, WB
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      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
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      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
        MCA928F

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              References

              1. Neumayer, R. et al. (1999) CD44 expression in benign and malignant colorectal polyps.
              Dis Colon Rectum. 42 (1): 50-5.
              2. Koopman, G. et al. (1993) Activated human lymphocytes and aggressive non-Hodgkin's lymphomas express a homologue of the rat metastasis-associated variant of CD44.
              J Exp Med. 177 (4): 897-904.
              3. Yoo, S.H. et al. (2005) Expression patterns of markers for type II pneumocytes in pulmonary sclerosing hemangiomas and fetal lung tissues.
              Arch Pathol Lab Med. 129 (7): 915-9.
              4. Kim, J.S. and Werth, V.P. (2011) Identification of specific chondroitin sulfate species in cutaneous autoimmune disease.
              J Histochem Cytochem. 59: 780-90.
              5. Chaiyarit, P. et al. (2008) Alteration of the expression of CD44 isoforms in oral epithelia and saliva from patients with oral lichen planus.
              J Clin Immunol. 28: 26-34.
              6. Shirure, V.S. et al. (2015) CD44 variant isoforms expressed by breast cancer cells are functional E-selectin ligands under flow conditions.
              Am J Physiol Cell Physiol. 308 (1): C68-78.

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