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CD44 antibody | Bu52

Product Code Applications Pack Size List Price Your Price Qty
MCA2504T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP P * 25 µg loader
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loader
MCA2504
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP P * 0.2 mg loader
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loader

Mouse anti Human CD44 antibody, clone Bu52 recognizes the human CD44 cell surface antigen, a ~100 kDa glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and of the breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. Mouse anti Human CD44 antibody, clone Bu52 recognizes an epitope that is common to all CD44 isoforms.

CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/100
Immunohistology - Frozen
Immunohistology - Paraffin 1 1/100 1/200
Immunoprecipitation
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Mouse IgG1 Negative Control

References for CD44 antibody

  1. Belitsos, P.C. et al. (1990) Homotypic cell aggregation induced by anti-CD44(Pgp-1) monoclonal antibodies and related to CD44(Pgp-1) expression.
    J Immunol. 144 (5): 1661-70.
  2. Katz, E. et al. (2012) Targeting of Rac GTPases blocks the spread of intact human breast cancer.
    Oncotarget. 3 (6): 608-19.
  3. Muerza-Cascante, M.L. et al. (2017) Endosteal-like extracellular matrix expression on melt electrospun written scaffolds.
    Acta Biomater. 52: 145-58.
  4. Hanke-Roos, M. et al. (2017) CD44 mediates the catch-bond activated rolling of HEPG2Iso epithelial cancer cells on hyaluronan.
    Cell Adh Migr. 11 (5-6): 476-87.
  5. Noda, S. et al. (2019) Effect of cell culture density on dental pulp-derived mesenchymal stem cells with reference to osteogenic differentiation.
    Sci Rep. 9 (1): 5430.
  6. Thorne, R.F. et al. (2020) Evaluating nuclear translocation of surface receptors: recommendations arising from analysis of CD44.
    Histochem Cell Biol. 153 (2): 77-87.
  7. Orikasa, S. et al. (2022) Hypoxia-inducible factor 1α induces osteo/odontoblast differentiation of human dental pulp stem cells via Wnt/β-catenin transcriptional cofactor BCL9.
    Sci Rep. 12 (1): 682.

Blocking Assay

Synonyms
H-CAM
PGP-1
RRID
AB_808430
UniProt
P16070
Entrez Gene
CD44

MCA2504T

1610

MCA2504

155368 1605 166452

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