CD43 Antibody | DFT-1

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CD43 Antibody | DFT-1 gallery image 1

Staining of human peripheral blood granulocytes with Mouse anti Human CD43:Biotin (MCA555B)

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CD43 Antibody | DFT-1 gallery image 2

Staining of human peripheral blood lymphocytes with Mouse anti Human CD43: FITC (MCA555F)

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CD43 Antibody | DFT-1 gallery image 3

Staining of human peripheral blood lymphocytes with Mouse anti Human CD43:Azide Free (MCA555XZ)

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CD43 Antibody | DFT-1 gallery image 4

Staining of human peripheral blood lymphocytes with Mouse anti Human CD43:RPE (MCA555PE)

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CD43 Antibody | DFT-1 gallery image 5

Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD43 antibody, clone DFT-1 (MCA555) followed by the HISTAR detection system (STAR3000). Low power

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CD43 Antibody | DFT-1 gallery image 6

Staining of human peripheral blood monocytes with Mouse anti Human CD43 (MCA555GA)

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CD43 Antibody | DFT-1 gallery image 7

Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD43 antibody, clone DFT-1 (MCA555) followed by the HISTAR detection system (STAR3000). Medium power

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CD43 Antibody | DFT-1 gallery image 8

Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD43 antibody, clone DFT-1 (MCA555) followed by the HISTAR detection system (STAR3000). Medium power

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CD43 Antibody | DFT-1 gallery image 9

Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD43 antibody, clone DFT-1 (MCA555) followed by the HISTAR detection system (STAR3000). High power

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CD43 Antibody | DFT-1 gallery image 10

Imunofluorescence staining of human tonsil cryosection with Mouse anti Human CD43, clone DFT-1 (MCA555), red in A and Mouse anti Human CD21 (MCA1195), green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power

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CD43 Antibody | DFT-1 gallery image 11

Imunofluorescence staining of human tonsil cryosection with Mouse anti Human CD43, clone DFT-1 (MCA555), red in A and Mouse anti Human CD21 (MCA1195), green in B. C is the merged image with nuclei counterstained blue using DAPI. Medium power

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CD43 Antibody | DFT-1 gallery image 12

Imunofluorescence staining of human tonsil cryosection with Mouse anti Human CD43, clone DFT-1 (MCA555), red in A and Mouse anti Human CD21 (MCA1195), green in B. C is the merged image with nuclei counterstained blue using DAPI. High power

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  • Mouse anti Human CD43
  • Mouse anti Human CD43
  • Mouse anti Human CD43:Alexa Fluor® 647
  • Mouse anti Human CD43:Alexa Fluor® 647
  • Mouse anti Human CD43:FITC
  • Mouse anti Human CD43:FITC
  • Mouse anti Human CD43:RPE
  • Mouse anti Human CD43:RPE
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    DFT-1
  • Isotype
    IgG1
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA555FFdatasheet pdfdatasheet pdf0.1 mg
    MCA555F
    MCA555C, F, Pdatasheet pdfdatasheet pdf0.2 mg
    MCA555
    MCA555PEFdatasheet pdfdatasheet pdf100 Tests
    MCA555PE
    MCA555A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA555A647
    MCA555TC, F, Pdatasheet pdfdatasheet pdf25 µg
    MCA555T
    MCA555FTFdatasheet pdfdatasheet pdf25 µg
    MCA555FT
    MCA555PETFdatasheet pdfdatasheet pdf25 Tests
    MCA555PET
    MCA555A647TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA555A647T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD43 antibody, clone DFT-1 recognizes human CD43 also known as leukosialin or sialophorin. CD43 is a 381 amino acid single pass type I transmembrane glycoprotein present on the cell surface of multiple cell types including thymocytes, T-lymphocytes, neutrophils, plasma cells and platelets. It is lacking on the surface of most B-lymphocytes. CD34 is a major membrane sialoglycoprotein expressed on T lymphocytes as two predominant glycoforms, a 115kDa form present on all T-cells and a 130kDa form indicitive of T-cell activation (Barren et al. 1997) and acts as a T-cell counter-receptor for CD169/Siglec-1 (van den Berg et al. 2001). Clone DFT-1 binds to both glycoforms of human CD43 (Kyoizumi et al. 2004)

      CD34 expression is deficient and/or defective in patients suffering from the X-linked chromocomal immunodeficiency condition Wiskott-Aldrich syndrome and acts as a ligand for CD54 (Rosenstein et al. 1991).

      Mouse anti human CD43, clone DFT-1 has been used successfully for the evaluation of CD43 expression in patients with Myelodysplastic syndromes (Kyriakou et al. 2006)
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
    • Immunogen
      KG-1 cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1 mg/ml
      IgG concentration 1 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      extracellular space
      establishment or maintenance of cell polarity
      leukocyte migration
      blood coagulation
      bacterial cell surface binding
      positive regulation of tumor necrosis factor biosynthetic process
      negative regulation of cell adhesion
      defense response to bacterium
      cellular defense response
      integral to plasma membrane
      transmembrane receptor activity
      chemotaxis
      immune response
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/100
      Immunohistology - Frozen
      Immunohistology - Paraffin1/101/100
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/100
      Immunohistology - Frozen
      Immunohistology - Paraffin1/101/100
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
      This product does not require protein digestion pre-treatment of paraffin sections however, staining of paraffin embedded formalin fixed tissues may be improved in some cases by enzyme pretreatment using Trypsin.
    • Immunohistology
      This product does not require protein digestion pre-treatment of paraffin sections however, staining of paraffin embedded formalin fixed tissues may be improved in some cases by enzyme pretreatment using Trypsin.
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Tonsil
    • Histology Positive Control Tissue
      Tonsil
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD43 Antibody Formats

    Formats Clone Applications Sizes available
    CD43 Antibody : RPE DFT-1 F 100 Tests | 25 Tests
    CD43 Antibody : Purified DFT-1 C, F, P 0.2 mg | 25 µg
    CD43 Antibody : FITC DFT-1 F 0.1 mg | 25 µg
    CD43 Antibody : Alexa Fluor® 647 DFT-1 F 100 Tests/1ml | 25 Tests/0.25ml
    • Copyright © 2016 Bio-Rad

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      STAR8D800GA
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      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
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      Recommended Negative Isotype Control

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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          Human SeroblockBUF070A50 TestF
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          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          Recommended Positive Controls

            Histology Controls

              Tonsil
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              References

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