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CD24 antibody | SN3

Product Code Applications Pack Size List Price Your Price Qty
MCA1379
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F 0.2 mg loader
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loader
MCA1379GA
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SDS Safety Datasheet SDS
C * F 0.1 mg loader
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loader

Mouse anti Human CD24 antibody, clone SN3 recognizes the human CD24 cell surface antigen, a heavily glycosylated molecule of ~35-45 kDa. CD24 is expressed by granulocytes and B lymphocytes, and by some activated T cells and T cell malignancies.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chimpanzee
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Glycoproteins purified from human NALM-1 cell line
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of a mouse myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen 1
Immunohistology - Paraffin
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood
Histology Positive Control Tissue
Tonsil

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
>
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Description Mouse IgG1 Negative Control

Source Reference

  1. Barcos, M. et al. (1986) Follicular mantle zone cell subpopulations detected by monoclonal antibody SN3.
    Hematol Oncol. 4 (4): 251-9.

References for CD24 antibody

  1. Fukukawa, T. et al. (1986) New monoclonal antibodies SN3, SN3a, and SN3b directed to sialic acid of glycoprotein on human non-T leukemia cells.
    Exp Hematol. 14 (9): 850-5.
  2. Ohyama, M. et al. (2006) Characterization and isolation of stem cell-enriched human hair follicle bulge cells.
    J Clin Invest. 116:249-60.
  3. Kap, Y.S. et al. (2009) A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
    J Histochem Cytochem. 57 (12): 1159-67.
  4. Sallustio, F. et al. (2010) TLR2 plays a role in the activation of human resident renal stem/progenitor cells.
    FASEB J. 24 (2): 514-25.
  5. Kanderová, V. et al. (2010) Aberrantly expressed CEACAM6 is involved in the signaling leading to apoptosis of acute lymphoblastic leukemia cells.
    Exp Hematol. 38: 653-660.e1.
  6. Franz, C. et al. (2016) Extracellular vesicles in human follicular fluid do not promote coagulation.
    Reprod Biomed Online. 33 (5): 652-5.

Flow Cytometry

RRID
AB_321526
UniProt
P25063
Entrez Gene
CD24
GO Terms
GO:0005886 plasma membrane
GO:0001666 response to hypoxia
GO:0001775 cell activation
GO:0001959 regulation of cytokine-mediated signaling pathway
GO:0002237 response to molecule of bacterial origin
GO:0002768 immune response-regulating cell surface receptor signaling pathway
GO:0004871 signal transducer activity
GO:0016055 Wnt receptor signaling pathway
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0007411 axon guidance
GO:0008629 induction of apoptosis by intracellular signals
GO:0009986 cell surface
GO:0031225 anchored to membrane
GO:0016337 cell-cell adhesion
GO:0016477 cell migration
GO:0019901 protein kinase binding
GO:0030296 protein tyrosine kinase activator activity
GO:0030856 regulation of epithelial cell differentiation
GO:0031295 T cell costimulation
GO:0032597 B cell receptor transport into membrane raft
GO:0032600 chemokine receptor transport out of membrane raft
GO:0032913 negative regulation of transforming growth factor-beta3 production
GO:0042104 positive regulation of activated T cell proliferation
GO:0042632 cholesterol homeostasis
GO:0043406 positive regulation of MAP kinase activity
GO:0043408 regulation of MAPKKK cascade
GO:0043627 response to estrogen stimulus
GO:0045121 membrane raft
GO:0045730 respiratory burst

MCA1379

153283 157290 160549 163382

MCA1379GA

149731

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