CD238 Antibody | BRIC203

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  • Mouse anti Human CD238
  • Mouse anti Human CD238
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    BRIC203
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1987F, H, IPdatasheet pdfdatasheet pdf0.2 mg
    MCA1987
    MCA1987TF, H, IPdatasheet pdfdatasheet pdf25 µg
    MCA1987T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD238 antibody, clone BRIC203 recognizes human CD238, also known as the kell blood group antigen Kpbc. The kell blood group antigens are carried on the ~93 kDa erythrocyte membrane glycoprotein.

      In hemagglutination tests, Mouse anti Human CD238 antibody, clone BRIC203 reacts with Kp (a-b+) but not Kp (a+b-). In addition Mouse anti Human CD238 antibody, clone BRIC203 will agglutinate Kp (a+b-c+) and Kp (a-b-c+) erythrocytes but not react with Ko erythrocytes.

      Weaker staining in flow cytometry is observed on erythroblasts derived from CD34+ hematopoietic progenitor cells, compared to Kp (a-b+) red blood cells.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
    • Immunogen
      Human erythrocytes.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Pack Size: 25 µgTRIS buffered glycine
      Pack Size: 0.2 mgPhosphate buffered saline
      Pack Size: 25 µgTRIS buffered glycine
      Pack Size: 0.2 mgPhosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c were fused with cells of a myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      metal ion binding
      protein binding
      vasoconstriction
      proteolysis
      plasma membrane
      metalloendopeptidase activity
      integral to membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Haemagglutination
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Haemagglutination
      Immunoprecipitation

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD238 Antibody Formats

    Formats Clone Applications Sizes available
    CD238 Antibody : Purified BRIC203 F, H, IP 0.2 mg | 25 µg
    • Copyright © 2016 Bio-Rad

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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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