CD227 Antibody | C595 (NCRC48)

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CD227 Antibody | C595 (NCRC48) gallery image 1

Immunofluorescence staining of MCF-7 cells with Mouse anti Human CD227:FITC (MCA1742F) (green), nuclei are counterstained with DAPI (blue)

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CD227 Antibody | C595 (NCRC48) gallery image 2

Sandwich ELISA analysis of CD227 expression using Mouse anti Human CD227, clone C595 (MCA1742) as a capture reagent and biotinylated Mouse anti Human CD227, clone VU-4H5 (1695-0028) as a detection reagent with purified breast cancer antigen (BioRad Catalog 22502313) as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Plasma and serum samples diluted 1:5 are indicated in green and orange respectively

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CD227 Antibody | C595 (NCRC48) gallery image 3

Sandwich ELISA analysis of CD227 expression using Mouse anti Human CD227, clone C595 (MCA1742) as a capture reagent and biotinylated Mouse anti Human CD227, clone 6A4 (BioRad Catalog 22508250) as a detection reagent with purified breast cancer antigen (BioRad Catalog 22502313) as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Plasma and serum samples diluted 1:5 are indicated in green and orange respectively

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  • Mouse anti Human CD227:FITC
  • Mouse anti Human CD227:FITC
  • Mouse anti Human CD227
  • Mouse anti Human CD227
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    C595 (NCRC48)
  • Isotype
    IgG3
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1742FF, IFdatasheet pdfdatasheet pdf0.1 mg
    MCA1742F
    MCA1742C, E, F, IF, P *, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA1742
    MCA1742FTF, IFdatasheet pdfdatasheet pdf25 µg
    MCA1742FT
    MCA1742TC, E, F, Pdatasheet pdfdatasheet pdf25 µg
    MCA1742T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD227 antibody, clone C595 (NCRC48) recognizes CD227, also known as mucin 1 which is a breast cancer associated mucin encoded by the Muc-1 gene. Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases.

      Mouse anti Human CD227 antibody, clone C595 recognizes the peptide epitope ARG-PRO-ALA-PRO within the protein core of the mucin.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein A
      Pack Size: 25 µgPurified IgG prepared by affinity chromatography on Protein G
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein A
      Pack Size: 25 µgPurified IgG prepared by affinity chromatography on Protein G
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
    • Immunogen
      Urinary MUC-1 mucin.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of a mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
      Immunofluorescence
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
      Immunofluorescence
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/1000
      Flow Cytometry
      Immunofluorescence
      Immunohistology - Frozen
      Western Blotting
      Immunohistology - Paraffin(1)
      (1)
      This product requires heat mediated antigen retrieval prior to staining of paraffin embedded material. Citrate buffer pH6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/1000
      Flow Cytometry
      Immunofluorescence
      Immunohistology - Frozen
      Western Blotting
      Immunohistology - Paraffin(1)
      (1)
      This product requires heat mediated antigen retrieval prior to staining of paraffin embedded material. Citrate buffer pH6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
      This product does not require protein digestion pre-treatment of paraffin sections.This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Breast carcinoma
    • Histology Positive Control Tissue
      Breast carcinoma
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
      This clone is suitable for use in Western blotting applications. Bio-Rad recommend the use of MCA1742 for this purpose.
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD227 Antibody Formats

    Formats Clone Applications Sizes available
    CD227 Antibody : Purified C595 (NCRC48) C, E, F, IF, P, P *, WB 25 µg | 0.2 mg
    CD227 Antibody : FITC C595 (NCRC48) F, IF 0.1 mg | 25 µg
    • Copyright © 2016 Bio-Rad

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      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
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      Recommended Negative Isotype Control

        Useful Reagents

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          Recommended Positive Controls

            Histology Controls

              Breast carcinoma
              Breast carcinoma
              • Application NameReference Images
                Flow Cytometry

              References

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