CD221 Antibody | 1H7

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CD221 Antibody | 1H7 gallery image 1

Staining of human peripheral blood granulocytes with Mouse anti Human CD221 (MCA2344)

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CD221 Antibody | 1H7 gallery image 2

Staining of human peripheral blood granulocytes with Mouse anti Human CD221: Alexa Fluor® 488 (MCA2344A488)

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CD221 Antibody | 1H7 gallery image 3

Staining of human peripheral blood granulocytes with Mouse anti Human CD221: Low Endotoxin (MCA2344EL)

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CD221 Antibody | 1H7 gallery image 4

Staining of human peripheral blood granulocytes with Mouse anti Human CD221: RPE (MCA2344PE)

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  • CD221 Antibody | 1H7 thumbnail image 1
  • CD221 Antibody | 1H7 thumbnail image 2
  • CD221 Antibody | 1H7 thumbnail image 3
  • CD221 Antibody | 1H7 thumbnail image 4
  • Mouse anti Human CD221:RPE
  • Mouse anti Human CD221:Alexa Fluor® 488
  • Mouse anti Human CD221:Alexa Fluor® 488
  • Mouse anti Human CD221
  • Mouse anti Human CD221:RPE
  • Mouse anti Human CD221
  • Mouse anti Human CD221:Low Endotoxin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    1H7
  • Isotype
    IgG1
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2344F, P *, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA2344
    MCA2344ELF, FN, P *, WBdatasheet pdfdatasheet pdf0.5 mg
    MCA2344EL
    MCA2344PEFdatasheet pdfdatasheet pdf100 Tests
    MCA2344PE
    MCA2344A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2344A488
    MCA2344TF, P *, WBdatasheet pdfdatasheet pdf25 µg
    MCA2344T
    MCA2344PETFdatasheet pdfdatasheet pdf25 Tests
    MCA2344PET
    MCA2344A488TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA2344A488T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD221 antibody, clone 1H7 recognizes human CD221, a ~155 kDa receptor tyrosine kinase, also known as Insulin-like growth factor I receptor (IGF-I Receptor). CD221 is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. Clone 1H7 recognizes an epitope in the alpha subunits of CD221, demonstrated by western blotting (Li et al.1993).

      CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin.

      The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signalling proteins. Clone 1H7 is reported to partially block binding of IGF-I and IGF-II to CD221 (Li et al.1993).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      None present
    • Immunogen
      Purified human placental IGF-I receptor.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.05 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 myeloma cell line.
    • Storage
      Prior to reconstitution store at +4oC.
      Following reconstitution store at +4oC.
      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      Following reconstitution store at +4oC.
      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      identical protein binding
      ATP binding
      integral to plasma membrane
      insulin binding
      protein tetramerization
      insulin receptor signaling pathway
      positive regulation of cell proliferation
      phosphatidylinositol-mediated signaling
      insulin receptor binding
      immune response
      positive regulation of cell migration
      positive regulation of DNA replication
      phosphatidylinositol 3-kinase binding
      phosphatidylinositol 3-kinase cascade
      microsome
      insulin receptor substrate binding
      metal ion binding
      insulin-like growth factor receptor activity
      protein autophosphorylation
      anti-apoptosis
      insulin-like growth factor I binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/10
      Western Blotting
      Immunohistology - Paraffin(1)1/101/40
      (1)
      This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/10
      Western Blotting
      Immunohistology - Paraffin(1)1/101/40
      (1)
      This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/10
      Functional Assays
      Western Blotting
      Immunohistology - Paraffin(1)1/101/40
      (1)
      This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Pancreas, placenta
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Pancreas, placenta
    • Histology Positive Control Tissue
      Pancreas, placenta
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD221 Antibody Formats

    Formats Clone Applications Sizes available
    CD221 Antibody : Purified 1H7 F, P *, WB 25 µg | 0.2 mg
    CD221 Antibody : Alexa Fluor® 488 1H7 F 100 Tests/1ml | 25 Tests/0.25ml
    CD221 Antibody : RPE 1H7 F 25 Tests | 100 Tests
    CD221 Antibody : Low Endotoxin 1H7 F, FN, P *, WB 0.5 mg
    • Copyright © 2016 Bio-Rad

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          Recommended Positive Controls

            Histology Controls

              Pancreas, placenta
              Pancreas, placenta
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              • Application NameReference Images
                Western Blotting

              References

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