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CD206 Antibody | 7-450
Staining of frozen Human Tonsil stained with Mouse anti Human CD206 (MCA2519GA)
Enlarge- Mouse anti Human CD206
- Mouse anti Human CD206
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Product TypeMonoclonal Antibody
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Clone7-450
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IsotypeIgG2a
1 Formats Available
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
More Images
References
Reviews
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- Mouse anti Human CD206 antibody, clone 7-450 recognizes human CD206, otherwise known as macrophage mannose receptor (MMR), a transmembrane protein and member of the multilectin family of pattern recognition receptors (PRRs), which mediates phagocytosis and endocytosis, and is modulated by a range of factors including cytokines, immunoglobulin receptors and pathogens.
CD206 has two extracellular lectin binding sites: the cysteine-rich domain binds to sulphated sugars of glycoproteins such as thyroid stimulating hormone, whilst the recognition of mannose and fucose occurs through carbohydrate recognition domains (CRDs). The widespread distribution and recognition of a wide range of antigens by macrophages, plays a key role in both innate and adaptive immunity, with CD206 able to bind to both gram-negative and gram-positive bacteria, mycobacterium, yeasts, fungi and parasites. The expression of CD206 is not restricted to macrophages, but is also present on immature dendritic cells (DCs), endothelial cells, kidney mesangial cells, retinal pigment epithelium and tracheal smooth muscle cells.
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Intended Use
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Target SpeciesHuman
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Product FormPurified IgG - liquidPurified IgG - liquid
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Reconstitution
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PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatantPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
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Preservative Stabilisers
0.09% Sodium Azide (NaN3) 0.09% Sodium Azide (NaN3) -
ImmunogenMonocyte-derived dendritic cells.
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Purity
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Approx. Protein ConcentrationsIgG concentration 1.0mg/mlIgG concentration 1.0mg/ml
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Reagents In The Kit
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Preparing The Antibody
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Test Principle
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Buffer SolutionPhosphate buffered salinePhosphate buffered saline
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Fusion PartnersSpleen cells from immunised Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
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StorageStore at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. -
Shelf Life18 months from date of despatch.18 months from date of despatch.
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GO Termsreceptor-mediated endocytosisintegral to plasma membranereceptor activitymannose binding
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UniProt
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Entrez Gene
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Acknowledgements
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RegulatoryFor research purposes only
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution Immunohistology - Frozen 1/50 1/500 Application Name Yes No Min Dilution Max Dilution Immunohistology - Frozen 1/50 1/500 - Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Technical Advice
- Recommended Protocol
- Recommended Protocol
- ELISA
- ELISA
- Immunohistology
- Immunohistology
- Histology Positive Control TissueHuman tonsil.
- Histology Positive Control TissueHuman tonsil.
- Immunofluorescence
- Immunofluorescence
- Western Blotting
- Western Blotting
- Instructions For Use
- Instructions For Use
Additional CD206 Antibody Formats
| Formats | Clone | Applications | Sizes available |
|---|---|---|---|
| CD206 Antibody : Purified | 7-450 | C | 0.2 mg | 0.1 mg |
- Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)
Recommended Secondary Antibody
Recommended Negative Isotype Control
Useful Reagents
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Histar Detection System | STAR3000B | 150 Tests | C, P | STAR3000B | |
| Histar Detection System | STAR3000A | 50 Tests | C, P | STAR3000A | |
| Histar Detection System | STAR3000C | 500 Tests | C, P | STAR3000C |
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Histar Detection System | STAR3000B | 150 Tests | C, P | STAR3000B | |
| Histar Detection System | STAR3000A | 50 Tests | C, P | STAR3000A | |
| Histar Detection System | STAR3000C | 500 Tests | C, P | STAR3000C |
Recommended Positive Controls
Histology Controls
Human tonsil.
Human tonsil.
Further Reading
1. Stahl, P.D. & Ezekowitz, R.A. (1998) The mannose receptor is a pattern recognition receptor involved in host defense.Curr Opin Immunol. 10 (1): 50-5.
1. Gordon, S. (1995) The macrophage.Bioessays. 17 (11): 977-86.
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Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
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