CD206 Antibody | 15-2

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CD206 Antibody | 15-2 gallery image 1

Published customer image:
Mouse anti Humaqn CD206 antibody, clone 15-2 used for the identification of M2 macrophages in culture by immunofluorescence.
Image caption:
Surface receptor expression and phagocytic activity of differentiated cells. G-LPS-Møs (A, C) and M-IL-4-Møs (B, D) were fixed with 5% formalin, then blocked and stained with anti- MHC class II (A, B) or anti-CD206 (C, D) antibodies. Merged images of fluorescence and counterstaining with DAPI show the expression of MHC class II (A; white arrows) on G-LPS-Møs and CD206 (D) on M-IL-4-Møs. Uptake of fluorescent latex beads in G-LPS-Møs (E) and M-IL-4-Møs (F). The cells were incubated with fluorescently labeled opsonized latex beads for 90 minutes. After washing with phosphate buffer, cells were stained with trypan blue. The fluorescent image is overlaid on phase contrast image of cells. Bars represent 50 µμm. The total fluorescence collectively derived from the internalized beads and cell surface-associated particles was quantified by measuring the fluorescent intensity using a Fluorescence Spectrometer (G). This assay was performed in quintuplicate and repeated two times. Statistical analysis was performed using the Student t-test. * P≤0.00001.

From: Yamane, K. and Leung, K.-P. (2016),
Rabbit M1 and M2 macrophages can be induced by human recombinant GM-CSF and M-CSF.
FEBS Open Bio. Accepted Author Manuscript. doi:10.1002/2211-5463.12101.

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  • CD206 Antibody | 15-2 thumbnail image 1
  • Mouse anti Human CD206
  • Mouse anti Human CD206
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    15-2
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2155C, F, IF, WB*datasheet pdfdatasheet pdf0.2 mg
    MCA2155
    MCA2155TC, F, IF, WB*datasheet pdfdatasheet pdf25 µg
    MCA2155T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD206 monoclonal antibody, clone 15-2 recognizes human macrophage mannose receptor 1, also known as CD206 or C-type lectin domain family 13 member D-like. CD206 is a ~175 kDa single pass type I transmembrane glycoprotein belonging to the group of pattern recognition receptors (Paveley et al. 2011). CD206 has multiple carbohydrate recognition motifs and acts as a receptor for bacteria, fungi and other pathogens (Ezekowitz et al. 1990). CD206 is predominantly expressed in tissue macrophages and dendritic cells (Engering et al. 1997) and can also be found in a subpopulation of endothelial cells (Pack et al. 2007) and sperm cells (Cardona-Maya et al. 2006). CD206 can also be detected in a soluble form in human plasma and is elevated in patients with acute sepsis (Rødgaard-Hansen et al. 2013).

      Mouse anti CD206, clone 15-2 has been used extensively to monitor mannose receptor modulation in macrophages treated with a wide range of cytokines and growth factors (Chang et al. 2003) and to indicate CD206 as a marker for alternative activation of macrophages (Joerink et al. 2011).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
    • Immunogen
      Purified human mannose receptor.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice where fused with cells of the SP2/0 Ag.14 mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      receptor-mediated endocytosis
      mannose binding
      receptor activity
      integral to plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunofluorescence
      Immunohistology - Frozen
      Western Blotting(1)
      (1)
      Clone 15-2 recognises a protein of approximately 175kDa under non-reducing conditions.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunofluorescence
      Immunohistology - Frozen
      Western Blotting(1)
      (1)
      Clone 15-2 recognises a protein of approximately 175kDa under non-reducing conditions.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD206 Antibody Formats

    Formats Clone Applications Sizes available
    CD206 Antibody : Purified 15-2 C, F, IF, WB* 0.2 mg | 25 µg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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