CD2 Antibody | LT2

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CD2 Antibody | LT2 gallery image 1

Staining of human peripheral blood lymphocytes with Mouse anti Human CD2:FITC (MCA1194F)

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CD2 Antibody | LT2 gallery image 2

Staining of human peripheral blood lymphocytes with Mouse anti Human CD2 (MCA1194)

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CD2 Antibody | LT2 gallery image 3

Staining of human peripheral blood lymphocytes with Mouse anti Human CD2 (MCA1194GA)

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CD2 Antibody | LT2 gallery image 4

Staining of human peripheral blood lymphocytes with Mouse anti Human CD2:Alexa Fluor® 647 (MCA1194A647)

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CD2 Antibody | LT2 gallery image 5

Staining of human peripheral blood lymphocytes with Mouse anti Human CD2:Biotin (MCA1194B)

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CD2 Antibody | LT2 gallery image 6

Staining of human peripheral blood lymphocytes with Mouse anti Human CD2:RPE (MCA1194PE)

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CD2 Antibody | LT2 gallery image 7

Staining of human peripheral blood lymphocytes with Mouse anti Human CD2:Low Endotoxin (MCA1194EL)

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  • CD2 Antibody | LT2 thumbnail image 1
  • CD2 Antibody | LT2 thumbnail image 2
  • CD2 Antibody | LT2 thumbnail image 3
  • CD2 Antibody | LT2 thumbnail image 4
  • CD2 Antibody | LT2 thumbnail image 5
  • CD2 Antibody | LT2 thumbnail image 6
  • CD2 Antibody | LT2 thumbnail image 7
  • Mouse anti Human CD2:Biotin
  • Mouse anti Human CD2:Low Endotoxin
  • Mouse anti Human CD2
  • Mouse anti Human CD2:Biotin
  • Mouse anti Human CD2
  • Mouse anti Human CD2:Alexa Fluor® 647
  • Mouse anti Human CD2:FITC
  • Mouse anti Human CD2
  • Mouse anti Human CD2:RPE
  • Mouse anti Human CD2:RPE
  • Mouse anti Human CD2:Alexa Fluor® 647
  • Mouse anti Human CD2:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    LT2
  • Isotype
    IgG2b
6 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1194BFdatasheet pdfdatasheet pdf0.1 mg
    MCA1194B
    MCA1194GAC *, F, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA1194GA
    MCA1194FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1194F
    MCA1194C *, F, IPdatasheet pdfdatasheet pdf0.2 mg
    MCA1194
    MCA1194ELC *, F, FN, IPdatasheet pdfdatasheet pdf0.5 mg
    MCA1194EL
    MCA1194PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1194PE
    MCA1194A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1194A647
    MCA1194TC *, F, IPdatasheet pdfdatasheet pdf25 µg
    MCA1194T
    MCA1194BTFdatasheet pdfdatasheet pdf25 µg
    MCA1194BT
    MCA1194FTFdatasheet pdfdatasheet pdf25 µg
    MCA1194FT
    MCA1194PETFdatasheet pdfdatasheet pdf25 Tests
    MCA1194PET
    MCA1194A647TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA1194A647T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD2 antibody, clone LT2 recognizes human CD2, a single chain transmembrane glycoprotein of 50 kDa, expressed by T cells, NK cells and thymocytes.

      CD2 is a receptor for CD58 (LFA-3), and is important in mediating the interaction between T cells and target cells or antigen presenting cells.

      Mouse anti Human CD2 antibody, clone LT2 inhibits NK cell mediated cytotoxicity, and ConA and PHA mediated blast transformation.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Rhesus Monkeyyes
      Chimpanzeeyes
      Cynomolgus monkeyyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to biotin - liquid.
      Purified IgG - liquid
      Purified IgG - liquid.
      Purified IgG conjugated to biotin - liquid.
      Purified IgG - liquid.
      Purified IgG conjugated to Alexa Fluor® 647 - liquid.
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid.
      Purified IgG - liquid.
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised.
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised.
      Purified IgG conjugated to Alexa Fluor® 647 - liquid.
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid.
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1 ml distilled water.
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water.
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A.
      Purified IgG prepared by affinity chromatography on Protein G
      Purified IgG prepared by affinity chromatography on Protein A.
      Purified IgG prepared by affinity chromatography on Protein A.
      Purified IgG prepared by affinity chromatography on Protein A.
      Purified IgG prepared by affinity chromatography on Protein A.
      Purified IgG prepared by affinity chromatography on Protein A.
      Purified IgG prepared by affinity chromatography on Protein A.
      Purified IgG prepared by affinity chromatography on Protein A.
      Purified IgG prepared by affinity chromatography on Protein A.
      Purified IgG prepared by affinity chromatography on Protein A.
      Purified IgG prepared by affinity chromatography on Protein A.
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      None present
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      Inert Bulking Reagent
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      Inert Bulking Reagent
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Normal human peripheral blood lymphocytes.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 1 mg/ml
      IgG concentration 1 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      integral to plasma membrane
      cell surface receptor linked signaling pathway
      leukocyte migration
      natural killer cell activation
      membrane raft polarization
      cell-cell adhesion
      blood coagulation
      regulation of T cell differentiation
      positive regulation of myeloid dendritic cell activation
      receptor activity
      T cell activation
      induction of apoptosis
    • UniProt
    • Entrez Gene
    • Acknowledgements
      The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Functional Assays
      Immunohistology - Paraffin
      Immunoprecipitation
      Immunohistology - Frozen(1)1/2001/400
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Paraffin
      Immunoprecipitation
      Immunohistology - Frozen(1)1/2001/400
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Paraffin
      Immunoprecipitation
      Immunohistology - Frozen(1)1/2001/400
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Paraffin
      Immunoprecipitation
      Immunohistology - Frozen(1)1/2001/400
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Human Tonsil
    • Histology Positive Control Tissue
      Human Tonsil
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Human Tonsil
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Human Tonsil
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD2 Antibody Formats

    Formats Clone Applications Sizes available
    CD2 Antibody : RPE LT2 F 25 Tests | 100 Tests
    CD2 Antibody : Biotin LT2 F 0.1 mg | 25 µg
    CD2 Antibody : Purified LT2 C *, F, IP 0.1 mg | 0.2 mg | 25 µg
    CD2 Antibody : Low Endotoxin LT2 C *, F, FN, IP 0.5 mg
    CD2 Antibody : FITC LT2 F 25 µg | 0.1 mg
    CD2 Antibody : Alexa Fluor® 647 LT2 F 25 Tests/0.25ml | 100 Tests/1ml
    • Copyright © 2016 Bio-Rad

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      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG2b:HRPHCA038P0.1 mgE
      HCA038P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      Human anti Mouse IgG2b:RPEHCA038PE100 TestsF
      HCA038PE

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative ControlMCA691100 TestsF
        MCA691
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative ControlMCA691100 TestsF
        MCA691
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative ControlMCA691100 TestsF
        MCA691
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative Control:Alexa Fluor® 647MCA691A647100 Tests/1mlF
        MCA691A647
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative Control:FITCMCA691F100 TestsF
        MCA691F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative ControlMCA691100 TestsF
        MCA691
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative Control:RPEMCA691PE100 TestsF
        MCA691PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative Control:RPEMCA691PE100 TestsF
        MCA691PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative Control:Alexa Fluor® 647MCA691A647100 Tests/1mlF
        MCA691A647
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative Control:FITCMCA691F100 TestsF
        MCA691F

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              Human Tonsil
              Human Tonsil
              Human Tonsil
              Human Tonsil

              References

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