CD177 Antibody | MEM-166

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CD177 Antibody | MEM-166 gallery image 1

Staining of human peripheral blood granulocytes with Mouse anti Human CD177:FITC (MCA2045F)

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CD177 Antibody | MEM-166 gallery image 2

Published customer image:
Mouse anti Human CD177 antibody, clone MEM-166 used for the evaluation of CD177 expression on polymorphonuclear neutrophils by flow cytometry on peripheral blood leukocytes.
Image caption:
Gating strategies to evalate the expression of surface markers

From:Johansson ÅC, Ohlsson S, Pettersson Å, Bengtsson AA, Selga D, Hansson M, Hellmark T.
Impaired phagocytosis and reactive oxygen species production in phagocytes is associated with systemic vasculitis.
Arthritis Res Ther. 2016 Apr 22;18(1):92. doi: 10.1186/s13075-016-0994-1..

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CD177 Antibody | MEM-166 gallery image 3

Staining of human peripheral blood granulocytes with Mouse anti Human CD177:RPE (MCA2045PE)

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CD177 Antibody | MEM-166 gallery image 4

Image A. FITC conjugated Mouse anti Human CD66b (MCA216F) and RPE conjugated Mouse IgG1 isotype control (MCA928F). Image B. FITC conjugated Mouse anti Human CD66b (MCA216F) and RPE conjugated Mouse anti Human CD177 (MCA2045PE). All experiments performed on human Peripheral blood derived myeloid cells in the presence of human SeroBlock (BUF070A).

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  • CD177 Antibody | MEM-166 thumbnail image 1
  • CD177 Antibody | MEM-166 thumbnail image 2
  • CD177 Antibody | MEM-166 thumbnail image 3
  • CD177 Antibody | MEM-166 thumbnail image 4
  • Mouse anti Human CD177:FITC
  • Mouse anti Human CD177:RPE
  • Mouse anti Human CD177:FITC
  • Mouse anti Human CD177:RPE
  • Mouse anti Human CD177
  • Mouse anti Human CD177
  • Mouse anti Human CD177
  • Mouse anti Human CD177:APC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    MEM-166
  • Isotype
    IgG1
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2045FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2045F
    MCA2045GAF, IP, WB*datasheet pdfdatasheet pdf0.1 mg
    MCA2045GA
    MCA2045F, IP, WB*datasheet pdfdatasheet pdf0.2 mg
    MCA2045
    MCA2045PEFdatasheet pdfdatasheet pdf100 Tests
    MCA2045PE
    MCA2045APCFdatasheet pdfdatasheet pdf100 Tests
    MCA2045APC
    MCA2045FTFdatasheet pdfdatasheet pdf25 µg
    MCA2045FT
    MCA2045TF, IP, WB*datasheet pdfdatasheet pdf25 µg
    MCA2045T
    MCA2045PETFdatasheet pdfdatasheet pdf25 Tests
    MCA2045PET
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD177 antibody, clone MEM-166 recognizes human CD177 (neutrophil glycoprotein NB1). The neutrophil NB1 antigen is expressed by 97% of the caucasian population. Antibodies against NB1 can be involved in neonatal alloimmune neutropenia (NAN).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Rhesus Monkeyyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid.
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised.
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid.
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised.
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1 ml distilled water.
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water.
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Reconstitute with 1ml distilled water
    • Preparation
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein A.
      Pack Size: 25 µgPurified IgG prepared by affinity chromatography on Protein G.
      Purified IgG prepared by affinity chromatography on Protein G.
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein A.
      Pack Size: 25 µgPurified IgG prepared by affinity chromatography on Protein G.
      Purified IgG prepared by affinity chromatography on Protein G.
      Pack Size: 0.1 mg, 25 µgPurified IgG prepared by affinity chromatography on Protein G
      Pack Size: 0.2 mgPurified IgG prepared by affinity chromatography on Protein A.
      Pack Size: 0.1 mg, 25 µgPurified IgG prepared by affinity chromatography on Protein G
      Pack Size: 0.2 mgPurified IgG prepared by affinity chromatography on Protein A.
      Pack Size: 0.1 mg, 25 µgPurified IgG prepared by affinity chromatography on Protein G
      Pack Size: 0.2 mgPurified IgG prepared by affinity chromatography on Protein A.
      Purified IgG prepared by affinity chromatography on Protein G.
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1.0%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1.0%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Human granulocytes.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1mg/ml
      IgG concentration 0.1mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at +4oC.
      DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of reconstitution.
    • GO Terms
      anchored to membrane
      leukocyte migration
      plasma membrane
      blood coagulation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunoprecipitation
      Western Blotting(1)Non-reducing conditions
      (1)
      MEM-166 recognizes CD177 under non-reducing conditions
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunoprecipitation
      Western Blotting(1)Non-reducing conditions
      (1)
      MEM-166 recognizes CD177 under non-reducing conditions
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunoprecipitation
      Western Blotting(1)Non-reducing conditions
      (1)
      MEM-166 recognizes CD177 under non-reducing conditions
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD177 Antibody Formats

    Formats Clone Applications Sizes available
    CD177 Antibody : RPE MEM-166 F 25 Tests | 100 Tests
    CD177 Antibody : Purified MEM-166 F, IP, WB* 25 µg | 0.1 mg | 0.2 mg
    CD177 Antibody : FITC MEM-166 F 25 µg | 0.1 mg
    CD177 Antibody : APC MEM-166 F 100 Tests
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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