CD161 Antibody | B199.2

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CD161 Antibody | B199.2 gallery image 1

Figure A. PE-A750 conjugated mouse anti human CD3 (MCA463P750) and A647 conjugated mouse IgG1 isotype control (MCA928A647). Figure B. PE-750 conjugated mouse anti human CD3 (MCA463P750) and A647 conjugated mouse anti human CD161 (MCA1855A647). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5™ cell analyzer

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CD161 Antibody | B199.2 gallery image 2

Published customer image:
Mouse anti Hyman CD161 antibody, clone B199.2 used for the evaluation of CD161 expression on peripheral blood mononuclear cells by flow cytometry.
Image caption:
Assessment of lymphocyte subsets in individuals carrying different CD161 genotypes. PBMC were isolated and stained with the antibody combinations CD161/CD3/CD4 or CD161/CD3/CD56. Analyses were performed in gated viable lymphocytes as defined by forward- and side-scatter characteristics. (A) CD161 expression patterns on CD3- NK cells. Representative dot-plots obtained after staining of cells from one TT, TC, and CC individual are shown. Boxes indicate the CD56brightCD161+CD3- NK cell subset and the frequency. The numbers indicate % positive cells in each quadrant. (B) Decreased frequency of CD3-CD56+ NK cells in CC carriers. PBMC from 27 TT, 51 TC, and 16 carriers of CC were analyzed. Results are expressed as % NK cells among gated lymphocytes; the mean value calculated for each cohort is indicated by the horizontal line; *p< 0.05 (ANOVA). (C) Ratios of CD56dim to CD56bright NK cells. The frequencies of CD56dimCD161+CD3- and CD56brightCD161+CD3- NK cells were determined in gated lymphocytes from 22 TT, 46 TC, and 15 carriers of CC. The horizontal broken line is an arbitrary cut-off value. (D) Level of CD161 expression. Data are expressed as mean fluorescence intensity/MFI on gated CD3-CD56+ NK cells. (E) Proportion of NK cells co-expressing CD161. Data are expressed as % CD161+ cells in gated CD3-CD56+ NK cells. (F) CD161 expression patterns on CD3+ T cells. The numbers indicate % positive cells in each quadrant. (G) Proportion of CD4+ T cells co-expressing CD161. (H) Proportion of CD8+ T cells co-expressing CD161dim or CD161bright.

From: Rother S, Hundrieser J, Pokoyski C, Kollrich S, Borns K, Blasczyk R, et al. (2015) The c.503T>C Polymorphism in the Human KLRB1 Gene Alters Ligand Binding and Inhibitory Potential of CD161 Molecules.
PLoS ONE 10(8): e0135682.

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CD161 Antibody | B199.2 gallery image 3

Published customer image:
Mouse anti Hyman CD161 antibody, clone B199.2 used for the evaluation of CD161 expression on transfected HEK-293 cells and peripheral blood mononuclear cells by western blotting and flow cytometry using phycoerythrin conjugated Mouse anti Human CD161 antibody clone B199.1 (MCA1855PE).
Image caption:
Characterization of 293 transfectants. 293 cells were “mock”-transfected with the empty pIRES2-AcGFP1 vector or vectors containing CD161 from a homozygous TT or CC individual. Analyses were performed after selection of stable transfectants. (A) Flow cytometry analysis of 293-mock, 293-CD161/TT, and 293-CD161/CC cells after staining with the PE-conjugated anti-CD161 mAb B199.2. Grey histograms represent staining with an isotype control mAb. The numbers represent mean fluorescence intensity obtained by anti-CD161 staining. (B) Detection of CD161 by Western-blotting. 293 transfectants were lysed, and protein lysates were separated by 9% SDS-PAGE. After transferring to PVDF membranes, samples were probed with the anti-CD161 mAb (B199.2). Binding of B199.2 was visualized by HRP-conjugated goat anti-mouse-IgG and ECL reagents. Protein loading was controlled by staining with a mAb against α-Actin. Representative results of at least two independent experiments are shown. Lysates of PBMC activated for five days with IL-12 were used as a further control to estimate the molecular weight of CD161 molecules.

From: Rother S, Hundrieser J, Pokoyski C, Kollrich S, Borns K, Blasczyk R, et al. (2015) The c.503T>C Polymorphism in the Human KLRB1 Gene Alters Ligand Binding and Inhibitory Potential of CD161 Molecules.
PLoS ONE 10(8): e0135682.

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CD161 Antibody | B199.2 gallery image 4

Figure A. PE-A750 conjugated mouse anti human CD3 (MCA463P750) and Pacific Blue conjugated mouse IgG1 isotype control (MCA928PB). Figure B. PE-A750 conjugated mouse anti human CD3 (MCA463P750) and Pacific Blue conjugated mouse anti human CD161 (MCA1855PB). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5™ cell analyzer

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CD161 Antibody | B199.2 gallery image 5

Published customer image:
Phycoerythrin conjugated Mouse anti Hyman CD161 antibody, clone B199.2 used for the evaluation of CD161 expression on transfected HEK-293 cells by flow cytometry.
Image caption:
Characterization of the ligand-binding domain of CD161/TT and CD161/CC receptors. (A) Fluorescence histograms obtained after incubation of 293-CD161/TT and -CD161/CC transfectants with control Ig-Fc (closed histograms) and LLT1-Fc or LLT1-Fc proteins multimerized by protein A (open histograms). Binding of the recombinant protein to CD161 was demonstrated by PE-conjugated goat anti-human IgG. (B) Dose-effect relationship of LLT1-Fc binding. 293-CD161/TT and -CD161/CC cells were incubated with increasing amounts of LLT1-Fc. Mean fluorescence intensity (MFI) was determined after a second incubation step with PE-conjugated goat anti-human IgG. Results represent mean values ± SEM calculated for three independent experiments; *p< 0.05 (Unpaired t-test). (C) Epitope-specific antibody competition in CD161/TT and -CC receptor variants. 293-CD161/TT and -CC cells were incubated with a fixed concentration (10 µg/ml) of PE-labelled anti-CD161 mAb B199.2 alone and in combination with increasing amounts of competing anti-CD161 mAb 191B8 (IgG2a) or the anti-pig MHC class-II mAb MSA3 as IgG2a isotype control. Samples were analysed by flow cytometry and PE-MFI was determined. Competition is shown as % binding of mAb B199.2 and was calculated as follows: MFI (B199.2 plus 191B8) / MFI (B199.2 plus MSA3) x 100. MFI obtained with B199.2 in the absence of competing antibody was set as 100%. Results represent mean values ± SD calculated for five independent experiments; *p<0.05 (Unpaired t-test).

From: Rother S, Hundrieser J, Pokoyski C, Kollrich S, Borns K, Blasczyk R, et al. (2015) The c.503T>C Polymorphism in the Human KLRB1 Gene Alters Ligand Binding and Inhibitory Potential of CD161 Molecules.
PLoS ONE 10(8): e0135682.

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  • Mouse anti Human CD161
  • Mouse anti Human CD161:RPE
  • Mouse anti Human CD161:Pacific Blue®
  • Mouse anti Human CD161:RPE
  • Mouse anti Human CD161
  • Mouse anti Human CD161:FITC
  • Mouse anti Human CD161
  • Mouse anti Human CD161:Alexa Fluor® 647
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  • Product Type
    Monoclonal Antibody
  • Clone
    B199.2
  • Isotype
    IgG1
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1855GAC, F, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA1855GA
    MCA1855FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1855F
    MCA1855C, F, IPdatasheet pdfdatasheet pdf0.2 mg
    MCA1855
    MCA1855PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1855PE
    MCA1855PBFdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1855PB
    MCA1855A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1855A647
    MCA1855TC, F, IPdatasheet pdfdatasheet pdf25 µg
    MCA1855T
    MCA1855PETFdatasheet pdfdatasheet pdf25 Tests
    MCA1855PET
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD161 antibody, clone B199.2 recognizes the human Killer cell lectin-like receptor subfamily B member 1, also known as CD161, C-type lectin domain family 5 member B, HNKR-P1a, NKR-P1A or Natural killer cell surface protein P1A. CD161 is a 225 amino acid ~25 kDa predicted molecular mass, single pass type II transmembrane glycoprotein with a single C-type lectin domain. CD161 is expressed by almost all NK cells and a subset of CD3+ve T cells (Lanier 1994).

      CD161, a member of the C-lectin is expressed as a disulphide bond-linked homodimeric cell surface protein, comprising two chains of ~40-44 kDa (Lanier et al. 1994). CD161 acts as a receptor for another c-type lectin, LLT1 with roles in the regulation of NK cell and T cell function (Aldemir et al. 2005).

      Mouse anti Human CD161 antibody, clone B199.2 cross-competes with and recognizes a similar epitope to the DX1 monoclonal antibody (Lanier et al. 1994).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
      Purified IgG conjugated to Pacific Blue - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor 647 - liquid
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Purified human NK cells cultured in IL-2 (Bennett et al. 1996)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse P2X63.Ag8.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch
    • GO Terms
      cell surface receptor linked signaling pathway
      plasma membrane
      sugar binding
      integral to membrane
      transmembrane receptor activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNt - 1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD161 Antibody Formats

    Formats Clone Applications Sizes available
    CD161 Antibody : Purified B199.2 C, F, IP 0.2 mg | 25 µg | 0.1 mg
    CD161 Antibody : RPE B199.2 F 100 Tests | 25 Tests
    CD161 Antibody : Pacific Blue® B199.2 F 100 Tests/1ml
    CD161 Antibody : Alexa Fluor® 647 B199.2 F 100 Tests/1ml
    CD161 Antibody : FITC B199.2 F 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry
                Immunohistology - Frozen

              References

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