CD151 Antibody | 11G5a

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CD151 Antibody | 11G5a gallery image 1

Staining of human peripheral blood platelets with Mouse anti Human CD151 (MCA1856)

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CD151 Antibody | 11G5a gallery image 2

Published customer image:
Mouse anti Human CD151 antibody, clone 11G5a used for the detection of CD151 positive microdomains on the surface and in intracellular vesicles in pseudovirus infected HeLa cells by immuneoelectron microscopy.
Image caption:
Virions associate with CD151 positive microdomains on the cell surface and in intracellular vesicles. (A) HeLa cells were fixed with paraformaldehyde and the cell surface was immunostained with a monoclonal anti-CD63 antibody and polyclonal anti-CD151 antibody. (B) HeLa cells were exposed to HPV16 pseudovirions for the indicated time periods, and fixed with paraformaldehyde. The cell surface was immunostained with polyclonal anti-L1 antibody (K75, red) and a monoclonal anti-CD151 antibody (sc-5275). Inserts display enlarged sections that are shown in the right column. (C) HeLa cells were exposed to HPV16 pseudovirions for 7 hours. Cells were fixed and permeabilized with methanol. Intracellular PsVs were immunostained with monoclonal anti-L1 antibody (L1-7, red) and CD151 with a polyclonal anti-CD151 antibody (sc-33123, green). Bars, 20 μm. (D) HeLa cells were infected with pseudovirions for 4 hours and then fixed with paraformaldehyde. Cell surface CD151 was immunolabeled with 10 nm gold particles. Pseudovirions and CD151 were visualized by electron microscopy. Bar, 200 nm.

From: Spoden G, Freitag K, Husmann M, Boller K, Sapp M, Lambert C, et al. (2008)
Clathrin- and Caveolin-Independent Entry of Human Papillomavirus Type 16—Involvement of Tetraspanin-Enriched Microdomains (TEMs).
PLoS ONE 3(10): e3313.

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CD151 Antibody | 11G5a gallery image 3

Published customer image:
Mouse anti Human CD151 antibody, clone 11G5a used for the detection of CD151 expression on the surface and in intracellular vesicles in pseudovirus infected HeLa cells by immunofluorescence.
Image caption:
Figure 8. Inhibition of HPV16 pseudovirus entry in HeLa cells.
(A) HeLa cells were treated with control and CD151 specific antibody as indicated. Entry of AlexaFluor conjugated transferrin (upper panels) or HPV16 PsVs (lower panels) was analyzed. Cells were fixed with MetOH and stained with monoclonal L1 (L1-7) antibody. (B) HeLa cells were transfected with control (upper panels) or CD151 siRNA (lower panels). Entry of AlexaFluor conjugated transferrin was analyzed. (C, D) HeLa cells were treated with siRNA and infected with HPV16 PsVs for 12 hours. Cells were either fixed and permeabilized with methanol and PsV uptake was analyzed by immunostaining with monoclonal L1 (L1-7, red) and polyclonal anti-CD151 antibodies (green) (C), or cells were fixed with paraformaldehyde and surface staining was performed with polyclonal L1 antibody (K75, red) and monoclonal anti-CD151 (green) antibody as indicated (D). Bars 20 μm.

From: Spoden G, Freitag K, Husmann M, Boller K, Sapp M, Lambert C, et al. (2008)
Clathrin- and Caveolin-Independent Entry of Human Papillomavirus Type 16—Involvement of Tetraspanin-Enriched Microdomains (TEMs).
PLoS ONE 3(10): e3313.

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CD151 Antibody | 11G5a gallery image 4

Published customer image:
Mouse anti Human CD151 antiboddy used for the evaluation of CD151 complex expression in HCCLM3 and HepG2 cell lysates by western blotting
Image caption:
Identification of tetraspanin CD151 network and functional analysis.
CD151-containing complexes were solubilized using the mild detergent, Brij97, and were isolated by immunoprecipitation using specific CD151 mAbs. Associated proteins were eluted using Triton X-100 and separated by SDS-PAGE (A). After in-gel trypsin digestion of proteins, the resulting peptides were analyzed using LC-MS/MS (B and C). The peptides were separated by nano-HPLC. Total ion count was measured and visualized on a chromatogram (upper panel). At a precise time (e.g. 55.33 min for CD151 and 67.18 min for integrin β1, dotted straight line), the mass spectrum obtained is shown (middle panel) in which a parent ion can be selected (e.g. m/z?=?643.3489 for CD151 and 1010.0838 for integrin β1, black arrow). Fragmentation of that parent ion led to MS/MS spectrum generation containing b and y ions, and thus sequence information of the parent ion (lower panel). The amino acid sequence can be deduced after searches in the NCBI database using the program SEQUEST. The putative sequence of the peptide is shown with associated Xcorr and ΔCn. This peptide sequence led to the identification of CD151 and integrin β1. These peptides were converted into a gene symbol and rearranged according to GO functional enrichment provided by DAVID website. Then, the “tetraspanin CD151 web” was organized by the cytoscape (Ver: 2.6.2, http://www.cytoscape.org/), searching target genes that interacted with CD151, and removing genes that were not contained in our target genes using a data source (NCBI Entrez EUtilities Web Service Client) (D). Gene ontology analysis of the 57 molecular partners was performed. They were classified as 13 categories of biological processes including protein folding and cell migration (MOES, TSP1, c-MET, NRP1, ITA6, ITB1 and GIPC1) according to categories of the “GO Biological Process” (E). Western blotting identified CD151 formed complexes with integrin α6 and β1 in HCCLM3 cells as well as HepG2 cells, but not in normal hepatocytes L-02 (F).

From: Devbhandari RP, Shi G-M, Ke A-W, Wu F-Z, Huang X-Y, Wang X-Y, et al. (2011)
Profiling of the Tetraspanin CD151 Web and Conspiracy of CD151/Integrin β1 Complex in the Progression of Hepatocellular Carcinoma.
PLoS ONE 6(9): e24901.

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CD151 Antibody | 11G5a gallery image 5

Published customer image:
Mouse anti Human CD151 antibody used for the evaluation of CD151 expression in HCCLM3 and HepG2 cells by immunofluorescence and western blotting
Image caption:
Location and expression of CD151/integrin β1 complex in HCC cells.
An immunofluorescence assay was performed. CD151 was stained with red and integrin β1 was stained with green. CD151 and integrin β1 co-localized on the plasma membrane in HCCLM3 cells (A). Western blotting revealed that the expression of CD151 in high metastatic HCCLM3 cells was highest and non-metastatic HepG2 cells presented the lowest levels of CD151 among six HCC cells. The results also demonstrated high expression of integrin β1 in six HCC cells lines (B). Flow cytometry analysis showed that the high proportion of integrin β1-positive cells were in six HCC cells (C). After transfection, HCCLM3-shRNA 2# was most efficient in downregulating the expression of integrin β1 at both the protein and mRNA levels (D). The level of integrin β1 expression remained unchanged in HCC interfered with CD151 shRNA, and vice versa (E).

From: Devbhandari RP, Shi G-M, Ke A-W, Wu F-Z, Huang X-Y, Wang X-Y, et al. (2011)
Profiling of the Tetraspanin CD151 Web and Conspiracy of CD151/Integrin β1 Complex in the Progression of Hepatocellular Carcinoma.
PLoS ONE 6(9): e24901.

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CD151 Antibody | 11G5a gallery image 6

Published customer image:
Mouse anti Human CD151 antibody used for the evaluation of CD151 expression in tumor tissue sections by immunohistochemistry.
Image caption:
Expression of CD151/integrin β1 complex in HCC patients by immunohistochemistry.
After identification by H&E staining (A1, B1, C1, D1, E1, F1, G1, H1, I1, and J1), CD151 was mostly located on the membrane of tumor cells, with comparable variation (A2, B2, C2, D2, E2, F2, G2, H2, I2, and J2) and strong integrin β1 expression in most HCC cells, stroma fibroblasts, and epithelial cells of the bile duct (A3, B3, C3, D3, E3, F3, G3, H3, I3, and J3). A and B refer to adjacent nontumoral tissue. Representative tumor tissues are shown :C and D high expression of CD151 and integrin β1, E and F high expression of CD151 and low expression of integrin β1, G and H high expression of integrin β1 and low expression of CD151, I and J low expression of CD151 and integrin β1.

From: Devbhandari RP, Shi G-M, Ke A-W, Wu F-Z, Huang X-Y, Wang X-Y, et al. (2011)
Profiling of the Tetraspanin CD151 Web and Conspiracy of CD151/Integrin β1 Complex in the Progression of Hepatocellular Carcinoma.
PLoS ONE 6(9): e24901.

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  • Mouse anti Human CD151:FITC
  • Mouse anti Human CD151
  • Mouse anti Human CD151
  • Mouse anti Human CD151
  • Mouse anti Human CD151:RPE
  • Mouse anti Human CD151:FITC
  • Mouse anti Human CD151:RPE
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    11G5a
  • Isotype
    IgG1
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1856FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1856F
    MCA1856GAC, E, F, IP, P *, WB*datasheet pdfdatasheet pdf0.1 mg
    MCA1856GA
    MCA1856C, E, F, IP, P *, WB*datasheet pdfdatasheet pdf0.2 mg
    MCA1856
    MCA1856PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1856PE
    MCA1856TC, E, F, IP, P *, WB*datasheet pdfdatasheet pdf25 µg
    MCA1856T
    MCA1856FTFdatasheet pdfdatasheet pdf25 µg
    MCA1856FT
    MCA1856PETFdatasheet pdfdatasheet pdf25 Tests
    MCA1856PET
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD151 antibody, clone 11G5a recognizes the human CD151 cell surface antigen, also known as PETA-3. CD151 is expressed by epithelial cells, endothelial cells, platelets, megakaryocytes, monocytes and in the renal glomeruli and proximal and distal tubules. CD151 is not expressed by lymphocytes or granulocytes. More recently CD151 has also been shown to be expressed by erythrocytes, and to carry the MER2 blood group antigen (Crew et al. 2004).

      It should be noted that CD151 is very closely associated with the alpha3 beta1 integrin in cells and co-immunoprecipitation may occur even in quite stringent conditions (Yauch. et al. 1998).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
    • Preparation
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein A
      Pack Size: 25 µgPurified IgG prepared by affinity chromatography on Protein G
      Pack Size: 0.2 mg, 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Pack Size: 0.2 mg, 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Pack Size: 0.2 mg, 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein A
      Pack Size: 25 µgPurified IgG prepared by affinity chromatography on Protein G
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      12 months from date of reconstitution.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry1/501/100
      Immunohistology - Frozen
      Immunoprecipitation
      Immunohistology - Paraffin(1)1/100
      Western Blotting(2)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
      (2)
      Clone 11G5a recognises human CD151 under non-reducing conditions.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry1/501/100
      Immunohistology - Frozen
      Immunoprecipitation
      Immunohistology - Paraffin(1)1/100
      Western Blotting(2)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
      (2)
      Clone 11G5a recognises human CD151 under non-reducing conditions.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry1/501/100
      Immunohistology - Frozen
      Immunoprecipitation
      Immunohistology - Paraffin(1)1/100
      Western Blotting(2)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
      (2)
      Clone 11G5a recognises human CD151 under non-reducing conditions.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Tonsil/spleen
    • Histology Positive Control Tissue
      Tonsil/spleen.
    • Histology Positive Control Tissue
      Tonsil/spleen.
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD151 Antibody Formats

    Formats Clone Applications Sizes available
    CD151 Antibody : Purified 11G5a C, E, F, IP, P *, WB* 0.2 mg | 25 µg | 0.1 mg
    CD151 Antibody : RPE 11G5a F 100 Tests | 25 Tests
    CD151 Antibody : FITC 11G5a F 0.1 mg | 25 µg
    • Copyright © 2016 Bio-Rad

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          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              Tonsil/spleen
              Tonsil/spleen.
              Tonsil/spleen.

              References

              Further Reading

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