CD147 Antibody | MEM-M6/6

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  • Mouse anti Human CD147:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    MEM-M6/6
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2882ZF, FN, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2882Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human CD147 antibody, clone MEM-M6/6 recognizes the Ig-like V-type domain of human CD147, otherwise known as basigin, neurothelin or extracellular matrix metalloproteinase inducer (EMMPRIN). CD147 is a 364 amino acid, ~50-55 kDa single pass type I transmembrane glycoprotein and member of the immunoglobulin gene superfamily, containing a single Ig-like C2-type and a single Ig-like V-type domain. CD147 is broadly expressed on peripheral blood cells, endothelial cells, and increased on T cells following activation. CD147 is also strongly expressed on small lung cancer cells and hepatocellular carcinoma.

      Biological properties of CD147 include the regulation of T cells, integrin functions and cell adhesion, the stimulation of metalloproteinase (MMP) production by fibroblasts, the promotion of astrocyte outgrowths, and roles in tumour progression, neuronal system function and reproduction (Muramatsu et al. 2003).

      There are four distinct isoforms of CD147 described in UniProt entry P35613 resulting from alternative splicing or differing promotor usage, the canonical version described here and three alternative truncated isoforms with alterations and deletions to the N-terminal region. The epitope recognized by mouse anti human CD147, clone MEM-M6/6 lies C-terminally to the altered sequences in these isoforms and clone MEM-M6/6 would therefore be expeceted to bind to all described forms of human CD147.

      Mouse anti Human CD147, clone MEM-M6/6 inhibits anti-CD3-induced T cell activation, dependent upon antibody concentration (Koch et al. 1999).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      None present
    • Immunogen
      CD147Rg, consisting of the cDNA coding for the extracellular region of CD147 expressed in CHO cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      leukocyte migration
      protein binding
      melanosome
      cell surface receptor linked signaling pathway
      Golgi membrane
      lactate transmembrane transporter activity
      blood coagulation
      integral to membrane
      pyruvate metabolic process
      mannose binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Functional Assays
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      MCA2882Z detects a band of approximately 45-60kDa in 293 human fibroblastoid cell lysates under non-reducing conditions.
    • Instructions For Use

    Additional CD147 Antibody Formats

    Formats Clone Applications Sizes available
    CD147 Antibody : Preservative Free MEM-M6/6 F, FN, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      STAR9B
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      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
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      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
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      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Functional Assays

              References

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