CD147 Antibody | MEM-M6/1

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CD147 Antibody | MEM-M6/1 gallery image 1

Staining of human erythrocytes with Mouse anti Human CD147 (MCA1876T)

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CD147 Antibody | MEM-M6/1 gallery image 2

Staining of human erythrocytes with Mouse anti Human CD147:APC (MCA1876APC)

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CD147 Antibody | MEM-M6/1 gallery image 3

Western blot analysis of HepG2 human hepatocellular carcinoma whole cell lysate probed with Mouse anti Human CD147 antibody (MCA1876) followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence

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  • CD147 Antibody | MEM-M6/1 thumbnail image 1
  • CD147 Antibody | MEM-M6/1 thumbnail image 2
  • CD147 Antibody | MEM-M6/1 thumbnail image 3
  • Mouse anti Human CD147:RPE
  • Mouse anti Human CD147:Preservative Free
  • Mouse anti Human CD147
  • Mouse anti Human CD147
  • Mouse anti Human CD147:APC
  • Mouse anti Human CD147:RPE
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    MEM-M6/1
  • Isotype
    IgG1
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1876F, IP, WB*datasheet pdfdatasheet pdf0.2 mg
    MCA1876
    MCA1876XZF, IP, WB*datasheet pdfdatasheet pdf1 mg
    MCA1876XZ
    MCA1876APCFdatasheet pdfdatasheet pdf100 Tests
    MCA1876APC
    MCA1876PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1876PE
    MCA1876TF, IPdatasheet pdfdatasheet pdf25 µg
    MCA1876T
    MCA1876PETFdatasheet pdfdatasheet pdf25 Tests
    MCA1876PET
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD147 antibody, clone MEM-M6/1 recognizes the human CD147 cell surface antigen, also known as Basigin, EMMPRIN and collagenase stimulatory factor. CD147 is a 385 amino acid single pass type 1 trans-membrane glycoprotein bearing 3 potential N-glycosylation sites, a single N-terminal (distal) Ig-like C2-type domain amd a more proximal Ig-like V-type domain in its extracellular region. Mouse anti Human CD147 antibody, clone MEM-M6/1 was raised and screened against recombinant extracellular region of huCD147 expressed in CHO cells, along with a number of other MEM-M6 clones. Epitope mapping indicates that clone MEM-M6/1 binds to an epitope within the distal Ig-like C-2 type domain. This is confirmed by flow cytometric studies using beads coated with constructs containing only the distal Ig-like or proximal Ig-like domains (Koch et al. 1999).

      Mouse anti Human CD147 antibody, clone MEM-M6/1 binds to both un-stimulated and phytohemagglutinin (PHA) stimulated (activated) T lymphocytes with levels of CD147 enhanced following PHA stimulation. CD147 is also expressed by peripheral blood and endothelial cells as well as many cultured cells of hematopoietic and non-hematopoietic origin (Koch et al. 1999). In addition to clone MEM-M6/1 Bio-Rad are also able to offer the MEM-M6/6 clone derived from the same fusion which recognizes an epitope in the membrane proximal Ig-like V type domain and has inhibitory effects on CD3 induced T cell activation.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1.0 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Reconstitute with 1ml distilled water
      Pack Size: 100 TestsReconstitute with 1.0 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
    • Preparation
      Purified IgG prepared by ion exchange chromatography
      Purified IgG prepared by hydrophobic charge induction chromatography.
      Pack Size: 25 µgPurified IgG prepared by affinity chromatography on Protein G
      Pack Size: 0.2 mgPurified IgG prepared by Protein A affinity chromatography.
      Pack Size: 25 µgPurified IgG prepared by affinity chromatography on Protein G
      Pack Size: 0.2 mgPurified IgG prepared by Protein A affinity chromatography.
      Purified IgG prepared by affinity chromatography on Protein A
      Purified IgG prepared by ion exchange chromatography
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      None present
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      CD147Rg, consisting of the cDNA coding for the extracellular region of human CD147.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1 mg/ml
      IgG concentration 1 mg/ml
      IgG concentration 1 mg/ml
      IgG concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized Balb/c mice were fused with SP2/0 mouse myeloma cells.
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at +4oC.
      DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      melanosome
      integral to membrane
      pyruvate metabolic process
      blood coagulation
      mannose binding
      Golgi membrane
      cell surface receptor linked signaling pathway
      leukocyte migration
      protein binding
      lactate transmembrane transporter activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunoprecipitation
      Western Blotting(1)
      (1)
      MEM-M6/1 recognizes CD147 under non-reducing conditions.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunoprecipitation
      Western Blotting(1)
      (1)
      MEM-M6/1 recognizes CD147 under non-reducing conditions.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunoprecipitation
      Western Blotting(1)
      (1)
      MEM-M6/1 recognizes CD147 under non-reducing conditions.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD147 Antibody Formats

    Formats Clone Applications Sizes available
    CD147 Antibody : RPE MEM-M6/1 F 100 Tests | 25 Tests
    CD147 Antibody : APC MEM-M6/1 F 100 Tests
    CD147 Antibody : Purified MEM-M6/1 F, IP, WB* 0.2 mg | 25 µg
    CD147 Antibody : Preservative Free MEM-M6/1 F, IP, WB* 1 mg
    • Copyright © 2016 Bio-Rad

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