CD104 Antibody | 450-9D

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CD104 Antibody | 450-9D gallery image 1

Staining of human tonsil cryostat section with Mouse anti human CD104 antibody, clone 450-9D (MCA1456).

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CD104 Antibody | 450-9D gallery image 2

Published customer image:
Mouse anti Human CD104 antibody, clone 450-9D (MCA1456) showing β4 integrin expression in reconstructed hemicorneal section by immunofluorescence.
Image caption:
Extract from Fig 1 showing β4 immunofluorescence:
Expression of epithelial cell markers in reconstructed hemicorneas. Human corneal epithelial stem cells seeded onto the HKL scaffold form a pluristratified and differentiated epithelium. Cryosections were analyzed through immunofluorescence. The layer of basal cuboidal cells was firmly attached to the underlying extra cellular matrix (ECM) and to the basement membrane through integrins α6 (blue, A), β1 (green, B), and β4 (red, C). The basal expression of p63 isoforms (red, D) and of the stem cell marker ΔNp63α (yellow, E) was always observed, thus suggesting the maintenance of undifferentiated progenitor cells interspersed between differentiated cells. Expression of keratin 14 (green, F) was also observed in the basal layers. Corneal differentiation occurred in all epithelial layers and was evaluated through the analysis of several markers, including 14-3-3σ (pink, G), keratin 19 (orange, H), keratin 3 (yellow, I), and keratin 12 (red, J). Note the presence of the Bowman's membrane in transmitted-light images (white arrows, D–E). Scale bar=50 μm.

From: Barbaro V, Ferrari S, Fasolo A, Ponzin D, Di Iorio E.
Reconstruction of a human hemicornea through natural scaffolds compatible with the growth of corneal epithelial stem cells and stromal keratocytes.
Mol Vis. 2009 Oct 17;15:2084-93.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

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  • CD104 Antibody | 450-9D thumbnail image 1
  • CD104 Antibody | 450-9D thumbnail image 2
  • Mouse anti Human CD104
  • Mouse anti Human CD104
  • Mouse anti Human CD104:FITC
  • Mouse anti Human CD104:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    450-9D
  • Isotype
    IgG1
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1456FF, IFdatasheet pdfdatasheet pdf0.1 mg
    MCA1456F
    MCA1456C*, F, IF, IP, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA1456
    MCA1456XZC*, F, IF, IP, WBdatasheet pdfdatasheet pdf1 mg
    MCA1456XZ
    MCA1456TC*, F, IF, IP, WBdatasheet pdfdatasheet pdf25 µg
    MCA1456T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD104 antibody, clone 450-9D recognizes the human beta4 integrin, also known as CD104. CD104 is a ~205 kDa glycoprotein which associates with the alpha6 integrin to form the alpha6/beta4 complex. CD104 is expressed on epithelial cells, Schwann cells and various tumor cell lines.

      Mouse anti Human CD104 antibody, clone 450-9D recognizes an extracellular epitope on the CD104 molecule.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mustelidyes
      Ferretyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      None present
    • Immunogen
      Purified alpha6 beta4 integrin from A431 cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Normal saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      hemidesmosome assembly
      cell communication
      integrin complex
      integrin-mediated signaling pathway
      protein binding
      cell-matrix adhesion
      multicellular organismal development
      receptor activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/100
      Immunofluorescence
      Immunohistology - Paraffin
      Immunoprecipitation
      Western Blotting
      Immunohistology - Frozen(1)1/5001/1000
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/100
      Immunofluorescence
      Immunohistology - Paraffin
      Immunoprecipitation
      Western Blotting
      Immunohistology - Frozen(1)1/5001/1000
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
      Immunofluorescence
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/100
      Immunofluorescence
      Immunohistology - Paraffin
      Immunoprecipitation
      Western Blotting
      Immunohistology - Frozen(1)1/5001/1000
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Human Tonsil
    • Histology Positive Control Tissue
      Human Tonsil
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Human Tonsil
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD104 Antibody Formats

    Formats Clone Applications Sizes available
    CD104 Antibody : FITC 450-9D F, IF 0.1 mg
    CD104 Antibody : Purified 450-9D C*, F, IF, IP, WB 0.2 mg | 25 µg
    CD104 Antibody : Preservative Free 450-9D C*, F, IF, IP, WB 1 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

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              References

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