CA125 Antibody | X325

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CA125 Antibody | X325 gallery image 1

Sandwich ELISA analysis of human CA-125 expression using Mouse anti Human CA-125 antibody, clone X75 (1695-0520) as a capture reagent and biotinylated Mouse anti Human CA-125 antibody, clone X325 (MCA2876) as a detection reagent with purified human CA-125 (Bio-rad Catalog: 83752310) as antigen to generate the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Plasma (green) sample at 1:2 is shown

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CA125 Antibody | X325 gallery image 2

Sandwich ELISA analysis of human CA-125 expression using Mouse anti Human CA-125 antibody, clone X325 (MCA2876) as a capture reagent and biotinylated Mouse anti Human CA-125 antibody, clone X75 (1695-0520) as a detection reagent with purified human CA-125 (Bio-rad Catalog: 83752310) as antigen to generate the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Plasma (green) sample at 1:2 is shown

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  • Mouse anti Human CA125
  • Mouse anti Human CA125
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    X325
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2876E, P, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA2876
    MCA2876GE, P, WBdatasheet pdfdatasheet pdf1 mg
    MCA2876G
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CA125 antibody, clone X325 recognizes the ovarian cancer mucin CA125 (also known as MUCIN 16). CA125 consists of a short cytoplasmic tail, a transmembrane domain and an exceptionally large glycosylated extracellular domain. The extracellular domain is dominated by a large number of 156-amino acid repeat units, over 60 in all. These repeats are known to bind the antibodies OC125 and M-11. CA125 is present within normal ovarian, endometrium, endocervix and fallopian tissue, but levels are elevated in over 90% of women with advanced ovarian cancer. CA125 levels may also be elevated in other cancers and non-cancerous conditions such as peritonitis, endometriosis and liver cirrhosis. Levels may also fluctuate during the menstrual cycle and occasionally increases in pregnancy, making CA125 an unreliable marker for ovarian cancer in premenopausal women. The function of CA125 in healthy tissue is unknown but it is thought to provide a protective lubricating barrier against particles and infectious agents at mucosal surfaces.

      CA125 was concluded by the International Society of Oncology and BioMarkers (ISOBM) to contain two major antigenic domains. Mouse anti Human CA125 antibody, clone X325 recognizes the epitope specificity group B, similar to the M-11 antibody (Nustad et al. 2002). Mouse anti Human CA125 antibody, clone X325 has been successfully as both a capture and detection reagent in the development of sensitive ELISA assays for the detection of CA125 antigen in human plasma samples.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by ion exchange chromatography
      Purified IgG prepared by ion exchange chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      CA125 antigen purified from human ovarian carcinoma.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
      TRIS buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      Avoid repeated freezing and thawing as this may denature the antibody.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Paraffin
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Paraffin
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Lung adenocarcinoma, serosal ovarian adenocarcinoma and urinary bladder adenocarcinoma tissue.
    • Histology Positive Control Tissue
      Lung adenocarcinoma, serosal ovarian adenocarcinoma and urinary bladder adenocarcinoma tissue.
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CA125 Antibody Formats

    Formats Clone Applications Sizes available
    CA125 Antibody : Purified X325 E, P, WB 0.2 mg | 1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
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      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
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      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
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      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
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      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
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      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
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      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
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      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
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      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
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      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
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      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
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      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
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      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
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      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Lung adenocarcinoma, serosal ovarian adenocarcinoma and urinary bladder adenocarcinoma tissue.
              Lung adenocarcinoma, serosal ovarian adenocarcinoma and urinary bladder adenocarcinoma tissue.

              References

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