C5 Antibody | 046-10.2.2 (10-04)

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  • Mouse anti Human C5
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    046-10.2.2 (10-04)
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2610E, FN, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2610
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • MCA2610 recognises secreted complement component 5 (C5), a 190kDa serine protease present in blood serum. It is composed of an 115kDa disulphide linked alpha-chain and a 75 kDa beta-chain. In addition to its role in complement, C5 is thought to be associated with inflammation, stimulating tumour necrosis factor alpha, interferon gamma mediated suppression of IL10 and IL12 production.

      C5 is activated via either the classical or the alternative complement pathway, by proteolytic cleavage of C5a from the N-terminal of the alpha-chain, by C5 convertase. C5a is an anaphylotoxin which mediates local inflammation. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. It also stimulates chemotaxis of neutrophils, eosinophils, basophils and monocytes towards the site of inflammation. The remaining fragment, C5b, initiates the assembly of the membrane attack complex.

      In haemolysis inhibition studies it was found that MCA2610 will block C5 haemolytic activity.

      Removal of sodium azide is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Functional Assays
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional C5 Antibody Formats

    Formats Clone Applications Sizes available
    C5 Antibody : Purified 046-10.2.2 (10-04) E, FN, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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