C3b Antibody | H11

C3b Antibody | H11 gallery image 1

Published customer image:
Mouse anti Human C3b antibody, clone H11 (0200-0614) used for the evaluation of C3b expression on CD59 positive and negative populations of human erythrocytes by flow cytometry.
Image caption:
In vivo C3 binding on red cells of PNH patients on eculizumab. a Kinetics of C3 binding on red cells from a representative PNH patient during eculizumab treatment. Before treatment (0 week), no red cell binds C3, whereas starting from 1 week of treatment three populations of red cells are displayed: one population of normal red cells (CD59?+?C3-) and two distinct populations of PNH (CD59-negative) red cells, one with (C3+) and one without (C3-) fragments bound on their surface. b Characterization of C3 fragment bound to erythrocytes from PNH patient on eculizumab. The left panel shows the normal (CD59-positive) and PNH (CD59-negative) populations (gated by the elliptic mark). The PNH (CD59-negative) population (see gate in the left panel) has been analyzed with anti-C3d and either anti-C3b (middle panel) or anti-iC3b (left panel). SSC: side scatter

From: Sica M, Rondelli T, Ricci P, De Angioletti M, Risitano AM, Notaro R.
Eculizumab treatment: stochastic occurrence of C3 binding to individual PNH erythrocytes.
J Hematol Oncol. 2017 Jun 19;10(1):126.

  • C3b Antibody | H11 thumbnail image 1
  • Mouse anti Human C3b
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    0200-0614C, WBdatasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Human C3b antibody, clone H11 recognizes human C3b, part of the complement system. C3 is cleaved by C3-convertase creating C3a and C3b, which binds to the surface of pathogens leading to their destruction by phagocytic cells. Mouse anti Human C3b antibody, clone H11 recognizes a determinant present on native (circulating) C3 and on the fragments C3b and C3c. The antibody is directed to a determinant present on the betachain of the C3 molecule (Western blot). It does not react with the anaphylatoxin C3a or the fragment C3d and does not inhibit the haemolytic function of C3.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - lyophilised
    • Reconstitution
      Reconstitute with 1.0 ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gentle mixing of the vial following reconstitution is recommended.
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      0.09% Sodium Azide, 0.5% Bovine Serum Albumin.
      These concentrations relate to the reconstituted product.
    • Immunogen
      Human C3b.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 50 ug/ml after reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
      receptor binding
      complement activation, alternative pathway
      inflammatory response
      endopeptidase inhibitor activity
      positive regulation vascular endothelial growth factor production
      G-protein coupled receptor protein signaling pathway
      complement activation, classical pathway
      extracellular space
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/10
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional C3b Antibody Formats

    Formats Clone Applications Sizes available
    C3b Antibody : Purified H11 C, WB 50 µg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


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