Apolipoprotein E Antibody | WUE-4

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Apolipoprotein E Antibody | WUE-4 gallery image 1

Human apolipoprotein E detected with Mouse anti Human apolipoprotein E:HRP (MCA5639P)

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Apolipoprotein E Antibody | WUE-4 gallery image 2

Human apolipoprotein E detected with Mouse anti Human apolipoprotein E (MCA5639) followed by Rabbit F(ab')2 anti Mouse IgG:HRP (STAR13B)

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Apolipoprotein E Antibody | WUE-4 gallery image 3

Sandwich ELISA analysis of human apolipoprotein E using Mouse anti Human apolipoprotein E (MCA5639) as a capture reagnt and biotinylated Goat anti Human Apolipoprotein E (AHP2177) as a detection reagent with purified apolipoprotein E as antigen. Detection is with Streptavidin HRP. A serum sample 1/150 (green) and a plasma sample 1/150 (orange) are shown. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135)

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  • Apolipoprotein E Antibody | WUE-4 thumbnail image 1
  • Apolipoprotein E Antibody | WUE-4 thumbnail image 2
  • Apolipoprotein E Antibody | WUE-4 thumbnail image 3
  • Mouse anti Human Apolipoprotein E
  • Mouse anti Human Apolipoprotein E:Biotin
  • Mouse anti Human Apolipoprotein E
  • Mouse anti Human Apolipoprotein E:Low Endotoxin
  • Mouse anti Human Apolipoprotein E:HRP
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  • Product Type
    Monoclonal Antibody
  • Clone
    WUE-4
  • Isotype
    IgG1
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5639BE, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA5639B
    MCA5639GAE, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA5639GA
    MCA5639PE, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA5639P
    MCA5639E, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA5639
    MCA5639ELE, FN, WBdatasheet pdfdatasheet pdf0.5 mg
    MCA5639EL
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human Apolipoprotein E antibody, clone WUE-4 recognizes an epitope within amino acids 140-160 of human apolipoprotein E (Apo-E), a major component of very low-density lipoproteins (VLDLs). Apo-E is the principle apolipoprotein in the central nervous system, and is secreted by most organs into the plasma, playing a vital role in the binding, internalization and catabolism of triglyceride-rich lipoprotein constituents.

      Apo-E acts as a ligand for both the specific apo-E receptor (chylomicron remnant) of hepatic tissues, and the apoB,E (LDL) receptor. Three isoforms of Apo-E have been identified, ApoE2, E3 and E4, and have been linked with various disorders. ApoE2 has been shown to bind LPL receptors with low affinity, resulting in increased plasma cholesterol and triglyceride levels, and thereby an increased risk in cardiovascular disorders. ApoE4 is a known high risk factor for Alzheimers disease, and in particular late onset Alzheimer disease 2 (AD2), whilst ApoE3 is the most common isoform, and considered the normal/natural Apo-E genotype.

      Mouse anti Human Apolipoprotein E antibody, clone WUE-4 has been shown to inhibit Apo-E mediated binding of lipoproteins to the apoB,E cell receptor (Krul et al. 1998).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/1000
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/1000
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/1000
      Functional Assays
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/1000
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      MCA5639 detects a major band of approximately 34-36kDa in human liver cell lysates.
    • Western Blotting
      MCA5639B detects a major band of approximately 34-36kDa in human liver cell lysates.
    • Western Blotting
      MCA5639GA detects a major band of approximately 34-36kDa in human liver cell lysates.
    • Western Blotting
      MCA5639EL detects a major band of approximately 34-36kDa in human liver cell lysates.
    • Western Blotting
      MCA5639P detects a major band of approximately 34-36kDa in human liver cell lysates.
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional Apolipoprotein E Antibody Formats

    Formats Clone Applications Sizes available
    Apolipoprotein E Antibody : Biotin WUE-4 E, WB 0.1 mg
    Apolipoprotein E Antibody : Purified WUE-4 E, WB 0.2 mg | 0.1 mg
    Apolipoprotein E Antibody : HRP WUE-4 E, WB 0.1 mg
    Apolipoprotein E Antibody : Low Endotoxin WUE-4 E, FN, WB 0.5 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
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      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
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      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
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      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
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      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
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      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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