This item has been added to your cart.
ACTH Antibody | 57 (BGN/1388/66)
Purchased this product? Why not be the first to submit a review and upload an image?
Enlarge- Mouse anti Human ACTH
- Mouse anti Human ACTH
-
Product TypeMonoclonal Antibody
-
Clone57 (BGN/1388/66)
-
IsotypeIgG1
1 Formats Available
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
More Images
References
Reviews
-
- Mouse anti Human ACTH antibody (Clone 57, BGN/1388/66) recognizes 1-24 ACTH (Synacthen) which, as a synthetic analogue of naturally-occurring Adrenocorticotropic Hormone, can be used to measure adrenal reserve and for the diagnosis of adrenal insufficiency by acute adrenocortical stimulation.
ACTH, released from the anterior pituitary gland in response to corticotropin-releasing hormone from the hypothalamus, acts on the adrenal cortex to stimulate the production of corticosteroids such as cortisol, involved in the response to stress. Studies have shown that the administration of 1-24 ACTH increases blood pressure, believed to be attributed to an increase in ACTH-stimulated cortisol secretion, in association with increased cardiac output.
Mouse anti Human ACTH antibody does not recognize 17-39 ACTH (CLIP). It does cross-reacts with rat N-terminal ACTH.
-
Intended Use
-
Target SpeciesHuman
-
Species Cross-Reactivity
N.B. Antibody reactivity and working conditions may vary between species.Target Species Cross Reactivity Rat 
-
Product FormPurified IgG - liquidPurified IgG - liquid
-
Reconstitution
-
PreparationPurified IgG prepared by affinity chromatography on Protein APurified IgG prepared by affinity chromatography on Protein A
-
Preservative Stabilisers
0.09% Sodium Azide (NaN3) 0.09% Sodium Azide (NaN3) -
ImmunogenSynthetic peptide corresponding to the amino acids 1 - 24 of the human ACTH conjugated to Keyhole Limpet Haemocyanin.
-
Peptide SequenceSYSMEHFRWGKPVGKKRRPVKVYP
-
Purity
-
Approx. Protein ConcentrationsIgG concentration 1.0mg/mlIgG concentration 1.0mg/ml
-
Reagents In The Kit
-
Preparing The Antibody
-
Test Principle
-
Buffer SolutionPhosphate buffered salinePhosphate buffered saline
-
StorageStore at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. -
Shelf Life18 months from date of despatch.18 months from date of despatch.
-
GO Termsgeneration of precursor metabolites and energystored secretory granulepositive regulation of gene-specific transcription from RNA polymerase II promotertype 1 melanocortin receptor bindinghormone activityregulation of blood pressuretype 3 melanocortin receptor bindingregulation of appetitecellular nitrogen compound metabolic processhormone biosynthetic processcell-cell signalingtype 4 melanocortin receptor bindingextracellular spacenegative regulation of tumor necrosis factor productionpeptide hormone processingcellular pigmentationneuropeptide signaling pathway
-
UniProt
-
Entrez Gene
-
Acknowledgements
-
RegulatoryFor research purposes only
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution ELISA Immunohistology - Paraffin(1) 1/100 1/200 (1)This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.Application Name Yes No Min Dilution Max Dilution ELISA Immunohistology - Paraffin(1) 1/100 1/200 (1)This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.- Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Technical Advice
- Recommended Protocol
- Recommended Protocol
- ELISA
- ELISA
- Immunohistology
- Immunohistology
- Histology Positive Control Tissue
- Histology Positive Control Tissue
- Immunofluorescence
- Immunofluorescence
- Western Blotting
- Western Blotting
- Instructions For Use
- Instructions For Use
Additional ACTH Antibody Formats
| Formats | Clone | Applications | Sizes available |
|---|---|---|---|
| ACTH Antibody : Purified | 57 (BGN/1388/66) | E, P * | 1 mg | 0.2 mg |
- Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)
Recommended Secondary Antibody
Recommended Negative Isotype Control
Useful Reagents
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Histar Detection System | STAR3000B | 150 Tests | C, P | STAR3000B | |
| Histar Detection System | STAR3000A | 50 Tests | C, P | STAR3000A | |
| Histar Detection System | STAR3000C | 500 Tests | C, P | STAR3000C |
Recommended Positive Controls
Histology Controls
References
1. Hatakeyama, H. et al. (2000) Functional adrenocorticotropic hormone receptor in cultured human vascular endothelial cells. Possible role in control of blood pressure. Hypertens. 36: 862-865.
2. Zhou, M et al. (2016) Localization of ATP-sensitive K+ channel subunits in rat pituitary gland.Arch Histol Cytol. 76 (1): 53-66.
You may also be interested in...
Formerly known as AbD Serotec, a global supplier and manufacturer of antibodies, kits, and reagents.
Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
Ordering
Payment methods we support:
- Invoice / Purchase Order
- Credit card
Follow us on
|
|
|
|
|
Also accessible on: |
||||
|
||||