IL-4 antibody | CC303
Mouse anti Bovine Interleukin-4
- Product Type
- Monoclonal Antibody
- Clone
- CC303
- Isotype
- IgG2a
- Specificity
- IL-4
Antibody Quality and Performance Guarantee
Mouse anti Bovine interleukin-4 antibody, clone CC303 (MCA1820) used for the identification of interferon-γ expressing dog lymphocytes by flow cytometry.
Image caption:
Representative dot plots illustrating the analysis of intracellular cytokine profile in T-cell subsets. (A) Pseudocolor plot distribution of short-term in vitro cultured (control or SLA-Ag stimulated) canine whole blood sample according to cell size (Forward scatter - FSC) and granularity (Side scatter- SSC) used for lymphocyte gate selection (R1) of FSCLowSSCLow events. (B) Pseudocolor plots representing cytokines + (IL-17, TNF-α, IFN-γ, TGF-β and IL-4) CD4+ cells within gated lymphocytes and (C) Pseudocolor plots representing cytokines + (IL-17, TNF-α, IFN-γ, TGF-β and IL-4) CD8+ cells within gated lymphocytes. The frequency of cytokines+ T-cells subsets were calculated by quadrant statistics approach and first reported as percentage of gated lymphocytes prior to the calculation of the SLAg/Control indexes.
From: Costa-Pereira C, Moreira ML, Soares RP, Marteleto BH, Ribeiro VM, França-Dias MH, Cardoso LM, Viana KF, Giunchetti RC, Martins-Filho OA, Araújo MS.
One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis.
BMC Vet Res. 2015 Apr 11;11(1):92.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine interleukin-4 antibody, clone CC303 (MCA1820) used for the detection of IL-4 expressing cells in yellow cattle and water buffalo following schistosome infection by flow cytometry.
Image caption:
Percentage of IFN-γ and IL-4 positive cells detected by flow cytometry pre and post infection. yellow cattle; (B) water buffalo. Level of significance when compared to water buffalo group at the same time, *p <0.05.
Yang J, Fu Z, Feng X, Shi Y, Yuan C, Liu J, Hong Y, Li H, Lu K, Lin J.
Comparison of worm development and host immune responses in natural hosts of Schistosoma japonicum, yellow cattle and water buffalo.
BMC Vet Res. 2012 Mar 13;8:25.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Filter by Application:
F Reset| Mouse anti Bovine Interleukin-4 antibody, clone CC303 is a monoclonal antibody that recognizes bovine interleukin‑4 (IL‑4), also known as B‑cell stimulatory factor 1. The antibody shows reported cross‑reactivity with IL‑4 from several other species, including dog, pig, sheep, mustelid, goat, dolphin, mink and fin whale (Pedersen et al. 2002 and Jaber et al. 2010). Antibody reactivity and optimal working conditions may vary between species. This antibody has been validated for use in ELISA and for intracellular staining of cells for flow cytometry. Interleukin‑4 is a glycosylated polypeptide cytokine expressed by several immune cell types, including T cells, granulocytes and mast cells. IL‑4 plays a key role in the regulation of bovine immune responses and is one of the defining cytokines of the Th2 lymphocyte phenotype. It contributes to immune regulation through activation of epithelial cells and by influencing cell proliferation and apoptosis. |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog Pig Sheep Mustelid Goat Dolphin Mink Fin Whale - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry 1 | |
1/50 | 1/100 |
| Western Blotting |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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Log in | ||
| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control | ||||||
References for IL-4 antibody
-
Pedersen, L.G. et al. (2002) Identification of monoclonal antibodies that cross-react with cytokines from different animal species.
Vet Immunol Immunopathol. 88 (3-4): 111-22. -
Aasted, B. et al. (2002) Cytokine profiles in peripheral blood mononuclear cells and lymph node cells from piglets infected in utero with porcine reproductive and respiratory syndrome virus.
Clin Diagn Lab Immunol. 9 (6): 1229-34. -
Jensen, P.V. et al. (2003) Cytokine profiles in adult mink infected with Aleutian mink disease parvovirus.
J Virol. 77: 7444-51. -
Dean, G.S. et al. (2005) Minimum infective dose of Mycobacterium bovis in cattle.
Infect Immun. 73 (10): 6467-71. -
Hamza, E. et al. (2007) Modulation of allergy incidence in icelandic horses is associated with a change in IL-4-producing T cells.
Int Arch Allergy Immunol. 144: 325-37. -
Taubert A et al. (2008) Antigen-induced cytokine production in lymphocytes of Eimeria bovis primary and challenge infected calves.
Vet Immunol Immunopathol. 126 (3-4): 309-20. -
Rutigliano, J.A. et al. (2008) Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection.
J Immunol Methods. 336: 71-7. -
Araújo, M.S. et al. (2009) T-cell-derived cytokines, nitric oxide production by peripheral blood monocytes and seric anti-Leishmania (Leishmania) chagasi IgG subclass patterns following immunization against canine visceral leishmaniasis using Leishvaccine and Leishmune.
Vaccine. 27 (7): 1008-17.
View The Latest Product References
-
Papadogiannakis, E.I. et al. (2009) Determination of intracellular cytokines IFN-gamma and IL-4 in canine T lymphocytes by flow cytometry following whole-blood culture.
Can J Vet Res. 73: 137-43. -
Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
Vet Immunol Immunopathol. 132:109-15. -
Nielsen, L. et al. (2009) Lymphotropism and host responses during acute wild-type canine distemper virus infections in a highly susceptible natural host.
J Gen Virol. 90: 2157-65. -
Jaber, J.R. et al. (2010) Cross-reactivity of anti-human, anti-porcine and anti-bovine cytokine antibodies with cetacean tissues.
J Comp Pathol. 143: 45-51. -
Araújo, M.S. et al. (2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.
Vet Immunol Immunopathol. 141: 64-75. -
Fellman, C.L. et al. (2011) Cyclosporine A affects the in vitro expression of T cell activation-related molecules and cytokines in dogs.
Vet Immunol Immunopathol. 140: 175-80. -
Yang, J. et al. (2012) Comparison of worm development and host immune responses in natural hosts of Schistosoma japonicum, yellow cattle and water buffalo.
BMC Vet Res. 8: 25. -
Geherin, S.A. et al. (2013) Ovine skin-recirculating γδ T cells express IFN-γ and IL-17 and exit tissue independently of CCR7.
Vet Immunol Immunopathol. 155 (1-2): 87-97. -
Costa-Pereira, C. et al. (2015) One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis.
BMC Vet Res. 11 (1): 92. -
Moreira, M.L. et al. (2016) Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes.
Vet Parasitol. 220: 33-45. -
Aguiar-Soares, R.D.O. et al. (2020) Phase I and II Clinical Trial Comparing the LBSap, Leishmune®, and Leish-Tec® Vaccines against Canine Visceral Leishmaniasis.
Vaccines (Basel). 8 (4)Nov 17 [Epub ahead of print]. -
Villa-Mancera, A. et al. (2021) Phage display-based vaccine with cathepsin L and excretory-secretory products mimotopes of Fasciola hepatica. induces protective cellular and humoral immune responses in sheep.
Vet Parasitol. 289: 109340. -
Blanco, C.F. et al. (2021) Semi-stable production of bovine IL-4 and GM-CSF in the mammalian episomal expression system
Journal of Veterinary Research. 65, Aug 19 [Epub ahead of print] -
Matralis, D.T. et al. (2023) Intracellular IFN-γ and IL-4 levels of CD4 + and CD8 + T cells in the peripheral blood of naturally infected (Leishmania infantum) symptomatic dogs before and following a 4-week treatment with miltefosine and allopurinol: a double-blinded, controlled and cross-sectional study.
Acta Vet Scand. 65 (1): 2. -
Dawson, H.D. et al. (2020) Porcine cytokines, chemokines and growth factors: 2019 update.
Res Vet Sci. 131: 266-300. -
Vieira, J.F.P. et al. (2022) CD4(+) T-lymphocytes from asymptomatic dogs infected with Leishmania infantum are able to activate macrophages for higher leishmanicidal ability in an in vitro co-culture experiment.
Mol Immunol. 151: 61-9.
- RRID
- AB_2127033
- UniProt
- P30367
- Entrez Gene
- IL4
- GO Terms
- GO:0005125 cytokine activity
- GO:0005136 interleukin-4 receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
- GO:0030890 positive regulation of B cell proliferation
- GO:0042113 B cell activation
- GO:0043066 negative regulation of apoptosis
- GO:0045348 positive regulation of MHC class II biosynthetic process
- View More GO Terms
- GO:0045671 negative regulation of osteoclast differentiation
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0048295 positive regulation of isotype switching to IgE isotypes
- GO:0048304 positive regulation of isotype switching to IgG isotypes
MCA1820
1608
161124
167390
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